Methanosarcina virus MetMV

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5K7NIT2|A0A5K7NIT2_9VIRU Uncharacterized protein OS=Methanosarcina virus MetMV OX=2488574 PE=4 SV=1
MM1 pKa = 7.34SKK3 pKa = 10.52VVLFTRR9 pKa = 11.84GNPWDD14 pKa = 3.78PSGIGSGTTSVLNTVSQTFSVPVTPQMKK42 pKa = 9.68FWLAGNGVSSVLITSARR59 pKa = 11.84LIQGTKK65 pKa = 10.23NIPVTVNGAASFYY78 pKa = 10.77IPATGKK84 pKa = 8.99WSDD87 pKa = 3.65YY88 pKa = 11.42ASISDD93 pKa = 4.1FSLPGNVTLTVTFSGTMNIATFLDD117 pKa = 3.66NSNPGDD123 pKa = 3.79PVVVSATEE131 pKa = 3.9IPSPEE136 pKa = 4.21TPSLAGEE143 pKa = 3.86ILFDD147 pKa = 4.02VEE149 pKa = 4.56FEE151 pKa = 4.34GSFVEE156 pKa = 4.69SVSGLMPSTIQNCTIEE172 pKa = 4.21TGNVLFGSGSCFCNGSEE189 pKa = 4.5SSPAVLIYY197 pKa = 9.56STNSTFVNRR206 pKa = 11.84CTEE209 pKa = 4.26DD210 pKa = 3.4NFTMSAFYY218 pKa = 10.57KK219 pKa = 9.74PLEE222 pKa = 4.35PFGEE226 pKa = 4.52YY227 pKa = 9.67PAGSRR232 pKa = 11.84IHH234 pKa = 5.84EE235 pKa = 3.77AVVFVKK241 pKa = 10.7VSGGYY246 pKa = 9.55NKK248 pKa = 9.9YY249 pKa = 10.58HH250 pKa = 6.31YY251 pKa = 10.91FNGTNFNDD259 pKa = 3.77PPFEE263 pKa = 4.29FEE265 pKa = 3.92SDD267 pKa = 3.3ASVEE271 pKa = 4.19THH273 pKa = 6.86LLNLMIGGAIQIIFDD288 pKa = 4.44GYY290 pKa = 10.47QIIKK294 pKa = 9.05GAVWTEE300 pKa = 3.93EE301 pKa = 4.15FTPPSQPFIFSSDD314 pKa = 3.28YY315 pKa = 11.51GLIEE319 pKa = 4.06LTSNSSITLTTNPAPGDD336 pKa = 3.84TIKK339 pKa = 11.21LKK341 pKa = 10.93GGTLGDD347 pKa = 3.54VTLTFVNYY355 pKa = 9.58YY356 pKa = 9.71PRR358 pKa = 11.84NQYY361 pKa = 8.92EE362 pKa = 4.21VQIGATVNEE371 pKa = 4.3TAANLTLAINRR382 pKa = 11.84IEE384 pKa = 4.17GAEE387 pKa = 4.11FTATATDD394 pKa = 3.51NVVDD398 pKa = 5.29IIAYY402 pKa = 9.84SPTFSTEE409 pKa = 3.48KK410 pKa = 9.97TGDD413 pKa = 3.97GITLSGVSSTLDD425 pKa = 3.29GLITEE430 pKa = 5.73DD431 pKa = 4.1ISVDD435 pKa = 3.53DD436 pKa = 4.68SFTVTDD442 pKa = 3.91GGKK445 pKa = 10.3AIQQNVSVTEE455 pKa = 4.82AIDD458 pKa = 3.71GLIDD462 pKa = 4.03GLSEE466 pKa = 4.47NINITDD472 pKa = 3.8YY473 pKa = 11.24FDD475 pKa = 3.68TSMDD479 pKa = 3.63GLDD482 pKa = 3.48EE483 pKa = 4.4SVLVSTSFVGLIDD496 pKa = 3.79VMGAVV501 pKa = 3.88

Molecular weight: 53.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5K7NMS3|A0A5K7NMS3_9VIRU Uncharacterized protein OS=Methanosarcina virus MetMV OX=2488574 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84KK3 pKa = 9.5KK4 pKa = 10.71LLEE7 pKa = 4.13KK8 pKa = 10.46LKK10 pKa = 10.84KK11 pKa = 9.84DD12 pKa = 3.45VKK14 pKa = 10.56KK15 pKa = 10.63YY16 pKa = 10.96GVMEE20 pKa = 3.79MSRR23 pKa = 11.84RR24 pKa = 11.84LRR26 pKa = 11.84WPYY29 pKa = 9.32MPLYY33 pKa = 10.59RR34 pKa = 11.84LINGKK39 pKa = 8.84NAGHH43 pKa = 6.61INLWDD48 pKa = 4.9RR49 pKa = 11.84IEE51 pKa = 4.79AYY53 pKa = 10.1YY54 pKa = 10.85KK55 pKa = 10.2KK56 pKa = 10.59AKK58 pKa = 9.65KK59 pKa = 9.96

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

18061

39

3002

228.6

25.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.084 ± 0.409

1.44 ± 0.239

5.919 ± 0.177

7.131 ± 0.434

3.455 ± 0.15

7.026 ± 0.367

1.55 ± 0.125

7.026 ± 0.238

7.691 ± 0.426

7.663 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.131

5.52 ± 0.331

3.754 ± 0.263

3.488 ± 0.189

4.319 ± 0.246

6.03 ± 0.333

6.262 ± 0.391

5.825 ± 0.221

1.528 ± 0.123

4.075 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski