Clostridium sp. CAG:440

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7GDC8|R7GDC8_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:440 OX=1262807 GN=BN658_00694 PE=4 SV=1
MM1 pKa = 7.32IKK3 pKa = 10.31EE4 pKa = 3.94NNEE7 pKa = 3.64EE8 pKa = 4.01FFKK11 pKa = 11.07NICQKK16 pKa = 11.15YY17 pKa = 8.09IDD19 pKa = 4.02TMSKK23 pKa = 10.68EE24 pKa = 4.19MLLEE28 pKa = 4.53ADD30 pKa = 3.98NKK32 pKa = 10.2KK33 pKa = 9.83ILYY36 pKa = 9.87IFIEE40 pKa = 4.25DD41 pKa = 3.86EE42 pKa = 4.28YY43 pKa = 11.21IEE45 pKa = 4.67LKK47 pKa = 10.71EE48 pKa = 4.26KK49 pKa = 11.02GSINIQNYY57 pKa = 9.68KK58 pKa = 10.73DD59 pKa = 3.8YY60 pKa = 11.29LDD62 pKa = 4.48GDD64 pKa = 4.12FTVYY68 pKa = 10.39QYY70 pKa = 11.83DD71 pKa = 3.94SYY73 pKa = 11.75QEE75 pKa = 4.22LYY77 pKa = 10.53KK78 pKa = 10.24VTIKK82 pKa = 11.13DD83 pKa = 3.55EE84 pKa = 4.04ILNDD88 pKa = 3.64IEE90 pKa = 5.48DD91 pKa = 4.47LGLFDD96 pKa = 6.98DD97 pKa = 4.18NNQWNFYY104 pKa = 8.82ITFEE108 pKa = 3.89EE109 pKa = 4.54LKK111 pKa = 10.41RR112 pKa = 11.84LEE114 pKa = 4.08YY115 pKa = 10.51GYY117 pKa = 10.71KK118 pKa = 9.17VQYY121 pKa = 10.92NYY123 pKa = 10.51TLLEE127 pKa = 3.97KK128 pKa = 10.87YY129 pKa = 10.14IIEE132 pKa = 4.3NEE134 pKa = 3.93EE135 pKa = 3.62DD136 pKa = 4.03FYY138 pKa = 10.98TYY140 pKa = 10.72FSLEE144 pKa = 3.69QLNEE148 pKa = 4.15FEE150 pKa = 4.98EE151 pKa = 4.69SLHH154 pKa = 7.02LYY156 pKa = 10.8YY157 pKa = 10.27EE158 pKa = 4.32TDD160 pKa = 5.45DD161 pKa = 3.57IDD163 pKa = 3.86TNKK166 pKa = 9.92WGEE169 pKa = 4.36LYY171 pKa = 10.87SKK173 pKa = 10.55SNKK176 pKa = 9.29YY177 pKa = 10.35FNNNIIALSEE187 pKa = 4.06GMISYY192 pKa = 11.11YY193 pKa = 10.92DD194 pKa = 3.76LLQDD198 pKa = 4.02YY199 pKa = 9.86KK200 pKa = 11.03INYY203 pKa = 5.69QQYY206 pKa = 10.08YY207 pKa = 9.9GLSLDD212 pKa = 3.72KK213 pKa = 10.92VIEE216 pKa = 4.08YY217 pKa = 10.19FRR219 pKa = 11.84EE220 pKa = 3.81NAIVNLMDD228 pKa = 3.75YY229 pKa = 11.12GSDD232 pKa = 3.32RR233 pKa = 11.84DD234 pKa = 3.79EE235 pKa = 4.97GLHH238 pKa = 5.7QLSSMYY244 pKa = 10.48KK245 pKa = 10.09EE246 pKa = 4.13IMDD249 pKa = 4.0NLGIKK254 pKa = 9.66YY255 pKa = 10.34SNVYY259 pKa = 9.27TEE261 pKa = 4.74DD262 pKa = 3.65VSDD265 pKa = 3.82GKK267 pKa = 11.24YY268 pKa = 8.99LTTITFEE275 pKa = 4.41DD276 pKa = 4.87DD277 pKa = 3.22YY278 pKa = 11.25SIEE281 pKa = 4.18VDD283 pKa = 3.32TSAWNGIEE291 pKa = 4.04VVTEE295 pKa = 4.25NIEE298 pKa = 4.43SIYY301 pKa = 10.8EE302 pKa = 4.03EE303 pKa = 4.48YY304 pKa = 10.57EE305 pKa = 4.01KK306 pKa = 10.88QSEE309 pKa = 4.22KK310 pKa = 10.37TKK312 pKa = 10.63QNEE315 pKa = 4.14VKK317 pKa = 10.55NEE319 pKa = 3.75KK320 pKa = 10.19EE321 pKa = 3.75FEE323 pKa = 4.04YY324 pKa = 11.1DD325 pKa = 3.64FNN327 pKa = 4.76

Molecular weight: 39.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7GCC1|R7GCC1_9CLOT Sporulation stage III protein AA OS=Clostridium sp. CAG:440 OX=1262807 GN=BN658_00370 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.41TNNNIVFYY11 pKa = 9.09GTGRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 10.29SSIARR22 pKa = 11.84VRR24 pKa = 11.84LVEE27 pKa = 4.01GSGKK31 pKa = 8.35ITINGKK37 pKa = 10.22DD38 pKa = 2.95IDD40 pKa = 4.17EE41 pKa = 4.57FFGLEE46 pKa = 3.93TLKK49 pKa = 11.23VIVRR53 pKa = 11.84QPLTVTNTTAKK64 pKa = 10.26YY65 pKa = 10.7DD66 pKa = 3.73VICTVKK72 pKa = 10.79GGGFTGQAGAIRR84 pKa = 11.84HH85 pKa = 6.08GIARR89 pKa = 11.84ALNEE93 pKa = 4.19ANSEE97 pKa = 4.02YY98 pKa = 10.53RR99 pKa = 11.84PSLKK103 pKa = 10.1TNGFLTRR110 pKa = 11.84DD111 pKa = 3.3PRR113 pKa = 11.84MKK115 pKa = 10.18EE116 pKa = 3.54RR117 pKa = 11.84KK118 pKa = 9.62KK119 pKa = 11.12YY120 pKa = 9.99GLKK123 pKa = 10.15KK124 pKa = 10.22ARR126 pKa = 11.84KK127 pKa = 8.46APQFSKK133 pKa = 10.93RR134 pKa = 3.46

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1420

0

1420

418409

29

5824

294.7

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.954 ± 0.06

1.089 ± 0.029

5.404 ± 0.053

8.08 ± 0.084

3.853 ± 0.064

5.712 ± 0.064

1.159 ± 0.025

10.209 ± 0.085

10.556 ± 0.082

8.2 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.034

7.534 ± 0.075

2.436 ± 0.035

2.922 ± 0.043

2.918 ± 0.048

5.808 ± 0.062

5.658 ± 0.102

6.016 ± 0.059

0.643 ± 0.019

4.503 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski