Black sea bass polyomavirus 1
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1C723|A0A0A1C723_9POLY SLT OS=Black sea bass polyomavirus 1 OX=1572341 PE=4 SV=1
MM1 pKa = 7.59 SEE3 pKa = 3.81 QSNSFEE9 pKa = 3.97 PRR11 pKa = 11.84 LRR13 pKa = 11.84 RR14 pKa = 11.84 ALGEE18 pKa = 4.04 HH19 pKa = 5.93 NNPRR23 pKa = 11.84 KK24 pKa = 9.72 SVRR27 pKa = 11.84 EE28 pKa = 3.87 CWEE31 pKa = 3.61 SWKK34 pKa = 10.94 AEE36 pKa = 4.07 QQKK39 pKa = 10.33 KK40 pKa = 9.87 PSLSEE45 pKa = 3.58 EE46 pKa = 3.74 DD47 pKa = 3.17 RR48 pKa = 11.84 GYY50 pKa = 10.83 GDD52 pKa = 4.56 SMVTLYY58 pKa = 10.35 EE59 pKa = 3.86 QYY61 pKa = 9.63 TQVEE65 pKa = 4.51 SALEE69 pKa = 4.1 SFSASQTPEE78 pKa = 3.77 KK79 pKa = 10.55 VSPVQFDD86 pKa = 3.48 EE87 pKa = 4.13 PLRR90 pKa = 11.84 EE91 pKa = 4.09 FLCSYY96 pKa = 11.01 AGFGHH101 pKa = 6.77 SYY103 pKa = 10.98 ILMCGEE109 pKa = 4.73 EE110 pKa = 4.06 KK111 pKa = 10.03 WKK113 pKa = 10.45 LYY115 pKa = 10.13 QRR117 pKa = 11.84 ILFTYY122 pKa = 7.9 RR123 pKa = 11.84 TEE125 pKa = 5.21 DD126 pKa = 3.35 RR127 pKa = 11.84 FCLSRR132 pKa = 11.84 PTPKK136 pKa = 9.79 GTVYY140 pKa = 10.8 YY141 pKa = 10.09 ISIKK145 pKa = 8.07 TRR147 pKa = 11.84 YY148 pKa = 9.3 RR149 pKa = 11.84 IGGDD153 pKa = 3.63 VVWNKK158 pKa = 9.67 CRR160 pKa = 11.84 AIGADD165 pKa = 3.09 SWFEE169 pKa = 3.71 LSAVKK174 pKa = 10.23 VKK176 pKa = 10.45 KK177 pKa = 9.88 YY178 pKa = 9.99 QQLKK182 pKa = 9.68 ACAFGLGGVEE192 pKa = 3.68 ITEE195 pKa = 4.31 GEE197 pKa = 4.41 LEE199 pKa = 4.23 FEE201 pKa = 4.41 RR202 pKa = 11.84 PPFKK206 pKa = 10.74 YY207 pKa = 10.42 ALLEE211 pKa = 4.29 KK212 pKa = 10.5 YY213 pKa = 10.15 AVEE216 pKa = 4.44 TGCCDD221 pKa = 3.63 PLLLLGDD228 pKa = 4.06 YY229 pKa = 9.36 LTFAEE234 pKa = 5.4 GLQSCDD240 pKa = 2.47 EE241 pKa = 4.77 CYY243 pKa = 10.27 QEE245 pKa = 4.28 QLRR248 pKa = 11.84 VADD251 pKa = 3.59 PAGRR255 pKa = 11.84 VHH257 pKa = 5.54 STRR260 pKa = 11.84 HH261 pKa = 4.96 EE262 pKa = 3.95 AHH264 pKa = 6.4 HH265 pKa = 5.97 QNAKK269 pKa = 9.78 GFKK272 pKa = 9.78 DD273 pKa = 3.58 VSNKK277 pKa = 9.57 KK278 pKa = 7.05 QQCVSACDD286 pKa = 3.8 VVCGCLRR293 pKa = 11.84 RR294 pKa = 11.84 RR295 pKa = 11.84 EE296 pKa = 4.3 TKK298 pKa = 9.43 MSRR301 pKa = 11.84 DD302 pKa = 3.38 DD303 pKa = 3.66 RR304 pKa = 11.84 FGARR308 pKa = 11.84 VLQGLEE314 pKa = 3.73 NLVRR318 pKa = 11.84 GVTTEE323 pKa = 3.86 MLSAAFLLKK332 pKa = 10.88 AMLPFPITEE341 pKa = 4.29 FFDD344 pKa = 4.09 KK345 pKa = 10.81 VVKK348 pKa = 10.06 TMVEE352 pKa = 4.1 AVPKK356 pKa = 8.69 KK357 pKa = 9.97 QGWVFRR363 pKa = 11.84 GPIDD367 pKa = 3.59 SGKK370 pKa = 8.59 STVAAAVCGLLGGVALNVNTNRR392 pKa = 11.84 DD393 pKa = 4.03 RR394 pKa = 11.84 LWVEE398 pKa = 3.92 LGRR401 pKa = 11.84 ALDD404 pKa = 3.46 RR405 pKa = 11.84 YY406 pKa = 8.75 MVVFEE411 pKa = 4.98 DD412 pKa = 3.63 VKK414 pKa = 11.06 GMPEE418 pKa = 3.87 RR419 pKa = 11.84 GHH421 pKa = 6.81 PEE423 pKa = 3.81 LPFGEE428 pKa = 4.77 GFPNLDD434 pKa = 3.72 SLRR437 pKa = 11.84 EE438 pKa = 4.02 CLDD441 pKa = 3.21 GLFKK445 pKa = 11.11 VGLEE449 pKa = 3.75 RR450 pKa = 11.84 KK451 pKa = 8.51 HH452 pKa = 4.93 QNKK455 pKa = 9.35 VEE457 pKa = 4.21 THH459 pKa = 5.76 FPPWIVTCNQYY470 pKa = 10.53 IIPEE474 pKa = 4.45 TILARR479 pKa = 11.84 CKK481 pKa = 10.83 VLDD484 pKa = 4.07 FAPGRR489 pKa = 11.84 LNFKK493 pKa = 10.55 QFVEE497 pKa = 4.24 QKK499 pKa = 10.6 GVDD502 pKa = 4.0 LRR504 pKa = 11.84 FMASGEE510 pKa = 4.24 CLLLIYY516 pKa = 10.75 CMFGDD521 pKa = 3.93 LTLFQGEE528 pKa = 4.22 QAVALSKK535 pKa = 10.65 QVSDD539 pKa = 4.23 EE540 pKa = 4.12 AALLPWGLASEE551 pKa = 4.73 GADD554 pKa = 3.51 TQDD557 pKa = 4.79 EE558 pKa = 4.32 EE559 pKa = 5.02 SQDD562 pKa = 3.7 STLSTIPVEE571 pKa = 4.44 CAPPSPGLRR580 pKa = 11.84 LEE582 pKa = 4.31 VRR584 pKa = 11.84 GPPHH588 pKa = 5.54 IRR590 pKa = 11.84 VRR592 pKa = 11.84 VRR594 pKa = 11.84 VSEE597 pKa = 4.16 PPAYY601 pKa = 9.62 RR602 pKa = 11.84 DD603 pKa = 3.47 PPPYY607 pKa = 9.93 PGPTGLQDD615 pKa = 3.64 LCSTAFTDD623 pKa = 4.81 DD624 pKa = 4.14 PEE626 pKa = 5.26 HH627 pKa = 6.65 LFSPGPPPFSPPDD640 pKa = 3.77 PGMHH644 pKa = 6.14 QGDD647 pKa = 4.62 SEE649 pKa = 4.66 PEE651 pKa = 3.88 SSPCEE656 pKa = 3.68 MVSIAEE662 pKa = 4.01 YY663 pKa = 10.4 KK664 pKa = 9.74 RR665 pKa = 11.84 RR666 pKa = 11.84 HH667 pKa = 5.95 NIGSDD672 pKa = 3.17 GLPNKK677 pKa = 9.81 RR678 pKa = 11.84 RR679 pKa = 11.84 RR680 pKa = 11.84 CLFVDD685 pKa = 5.81 DD686 pKa = 5.04 EE687 pKa = 4.62 AQCTDD692 pKa = 3.82 DD693 pKa = 3.61 EE694 pKa = 4.89 SEE696 pKa = 4.18 EE697 pKa = 4.13 EE698 pKa = 4.21 EE699 pKa = 4.4 EE700 pKa = 4.54 EE701 pKa = 4.61 EE702 pKa = 4.63 EE703 pKa = 5.5 DD704 pKa = 3.93 EE705 pKa = 5.66 QPSCSQRR712 pKa = 11.84 PTTPVTPARR721 pKa = 11.84 THH723 pKa = 5.29 QRR725 pKa = 11.84 PITDD729 pKa = 4.55 FLRR732 pKa = 11.84 QPLHH736 pKa = 6.75 PEE738 pKa = 4.24 GSKK741 pKa = 10.84 CPYY744 pKa = 9.9 KK745 pKa = 10.75 DD746 pKa = 3.09 AGCFFDD752 pKa = 4.69 HH753 pKa = 7.48 PVRR756 pKa = 11.84 PWCDD760 pKa = 2.47 RR761 pKa = 11.84 CLRR764 pKa = 11.84 DD765 pKa = 2.94 WVTPEE770 pKa = 3.6 KK771 pKa = 10.65 VPRR774 pKa = 11.84 PGTPTPPSVV783 pKa = 3.03
Molecular weight: 88.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.225
IPC2_protein 5.308
IPC_protein 5.283
Toseland 5.308
ProMoST 5.461
Dawson 5.321
Bjellqvist 5.436
Wikipedia 5.207
Rodwell 5.258
Grimsley 5.258
Solomon 5.321
Lehninger 5.283
Nozaki 5.474
DTASelect 5.601
Thurlkill 5.359
EMBOSS 5.296
Sillero 5.55
Patrickios 3.986
IPC_peptide 5.334
IPC2_peptide 5.55
IPC2.peptide.svr19 5.522
Protein with the highest isoelectric point:
>tr|A0A0A1C950|A0A0A1C950_9POLY VP2 OS=Black sea bass polyomavirus 1 OX=1572341 GN=VP2 PE=4 SV=1
MM1 pKa = 7.59 SEE3 pKa = 3.81 QSNSFEE9 pKa = 3.97 PRR11 pKa = 11.84 LRR13 pKa = 11.84 RR14 pKa = 11.84 ALGEE18 pKa = 4.04 HH19 pKa = 5.93 NNPRR23 pKa = 11.84 KK24 pKa = 9.72 SVRR27 pKa = 11.84 EE28 pKa = 3.87 CWEE31 pKa = 3.61 SWKK34 pKa = 10.94 AEE36 pKa = 4.07 QQKK39 pKa = 10.33 KK40 pKa = 9.87 PSLSEE45 pKa = 3.58 EE46 pKa = 3.74 DD47 pKa = 3.17 RR48 pKa = 11.84 GYY50 pKa = 10.83 GDD52 pKa = 4.56 SMVTLYY58 pKa = 10.35 EE59 pKa = 3.86 QYY61 pKa = 9.63 TQVEE65 pKa = 4.51 SALEE69 pKa = 4.1 SFSASQTPEE78 pKa = 3.77 KK79 pKa = 10.55 VSPVQFDD86 pKa = 3.48 EE87 pKa = 4.13 PLRR90 pKa = 11.84 EE91 pKa = 4.09 FLCSYY96 pKa = 11.01 AGFGHH101 pKa = 6.77 SYY103 pKa = 10.98 ILMCGEE109 pKa = 4.73 EE110 pKa = 4.06 KK111 pKa = 10.03 WKK113 pKa = 10.45 LYY115 pKa = 10.13 QRR117 pKa = 11.84 ILFTYY122 pKa = 7.9 RR123 pKa = 11.84 TEE125 pKa = 5.21 DD126 pKa = 3.35 RR127 pKa = 11.84 FCLSRR132 pKa = 11.84 PTPKK136 pKa = 9.79 GTVYY140 pKa = 10.8 YY141 pKa = 10.09 ISIKK145 pKa = 8.07 TRR147 pKa = 11.84 YY148 pKa = 9.3 RR149 pKa = 11.84 IGGDD153 pKa = 3.63 VVWNKK158 pKa = 9.67 CRR160 pKa = 11.84 AIGADD165 pKa = 3.09 SWFEE169 pKa = 3.71 LSAVKK174 pKa = 10.23 VKK176 pKa = 10.45 KK177 pKa = 9.88 YY178 pKa = 9.99 QQLKK182 pKa = 9.68 ACAFGLGGVEE192 pKa = 3.68 ITEE195 pKa = 4.31 GEE197 pKa = 4.41 LEE199 pKa = 4.23 FEE201 pKa = 4.41 RR202 pKa = 11.84 PPFKK206 pKa = 10.74 YY207 pKa = 10.42 ALLEE211 pKa = 4.29 KK212 pKa = 10.5 YY213 pKa = 10.15 AVEE216 pKa = 4.44 TGCCDD221 pKa = 3.63 PLLLLGDD228 pKa = 4.06 YY229 pKa = 9.36 LTFAEE234 pKa = 5.4 GLQSCDD240 pKa = 2.47 EE241 pKa = 4.77 CYY243 pKa = 10.27 QEE245 pKa = 4.28 QLRR248 pKa = 11.84 VADD251 pKa = 3.59 PAGRR255 pKa = 11.84 VHH257 pKa = 5.54 STRR260 pKa = 11.84 HH261 pKa = 4.96 EE262 pKa = 3.95 AHH264 pKa = 6.4 HH265 pKa = 5.97 QNAKK269 pKa = 9.78 GFKK272 pKa = 9.78 DD273 pKa = 3.58 VSNKK277 pKa = 9.57 KK278 pKa = 7.05 QQCVSACDD286 pKa = 3.8 VVCGCLRR293 pKa = 11.84 RR294 pKa = 11.84 RR295 pKa = 11.84 EE296 pKa = 4.3 TKK298 pKa = 9.43 MSRR301 pKa = 11.84 DD302 pKa = 3.38 DD303 pKa = 3.66 RR304 pKa = 11.84 FGARR308 pKa = 11.84 VLQGLEE314 pKa = 3.73 NLVRR318 pKa = 11.84 GVTTEE323 pKa = 3.86 MLSAAFLLKK332 pKa = 10.88 AMLPFPITEE341 pKa = 4.29 FFDD344 pKa = 4.09 KK345 pKa = 10.81 VVKK348 pKa = 10.06 TMVEE352 pKa = 4.1 AVPKK356 pKa = 8.69 KK357 pKa = 9.97 QGWVFRR363 pKa = 11.84 GPIDD367 pKa = 3.59 SGKK370 pKa = 8.59 STVAAAVCGLLGGVALNVNTNRR392 pKa = 11.84 DD393 pKa = 4.03 RR394 pKa = 11.84 LWVEE398 pKa = 3.92 LGRR401 pKa = 11.84 ALDD404 pKa = 3.46 RR405 pKa = 11.84 YY406 pKa = 8.75 MVVFEE411 pKa = 4.98 DD412 pKa = 3.63 VKK414 pKa = 11.06 GMPEE418 pKa = 3.87 RR419 pKa = 11.84 GHH421 pKa = 6.81 PEE423 pKa = 3.81 LPFGEE428 pKa = 4.77 GFPNLDD434 pKa = 3.72 SLRR437 pKa = 11.84 EE438 pKa = 4.02 CLDD441 pKa = 3.21 GLFKK445 pKa = 11.11 VGLEE449 pKa = 3.75 RR450 pKa = 11.84 KK451 pKa = 8.51 HH452 pKa = 4.93 QNKK455 pKa = 9.35 VEE457 pKa = 4.21 THH459 pKa = 5.76 FPPWIVTCNQYY470 pKa = 10.53 IIPEE474 pKa = 4.45 TILARR479 pKa = 11.84 CKK481 pKa = 10.83 VLDD484 pKa = 4.07 FAPGRR489 pKa = 11.84 LNFKK493 pKa = 10.55 QFVEE497 pKa = 4.24 QKK499 pKa = 10.6 GVDD502 pKa = 4.0 LRR504 pKa = 11.84 FMASGEE510 pKa = 4.24 CLLLIYY516 pKa = 10.75 CMFGDD521 pKa = 3.93 LTLFQGEE528 pKa = 4.22 QAVALSKK535 pKa = 10.65 QVSDD539 pKa = 4.23 EE540 pKa = 4.12 AALLPWGLASEE551 pKa = 4.73 GADD554 pKa = 3.51 TQDD557 pKa = 4.79 EE558 pKa = 4.32 EE559 pKa = 5.02 SQDD562 pKa = 3.7 STLSTIPVEE571 pKa = 4.44 CAPPSPGLRR580 pKa = 11.84 LEE582 pKa = 4.31 LSLMILNIFFLLVPPLFLLLILACIRR608 pKa = 11.84 ATLNLSRR615 pKa = 11.84 HH616 pKa = 4.99 PVRR619 pKa = 11.84 WSPSQSTSAAIILGQMVYY637 pKa = 9.68 PINVGVVSSWMTRR650 pKa = 11.84 HH651 pKa = 5.95 SAPMMRR657 pKa = 11.84 ARR659 pKa = 11.84 RR660 pKa = 11.84 KK661 pKa = 8.29 RR662 pKa = 11.84 RR663 pKa = 11.84 RR664 pKa = 11.84 RR665 pKa = 11.84 RR666 pKa = 11.84 TSNPVVPSVPPPQSPLPGPTNVLSRR691 pKa = 11.84 IFSVSLSTLKK701 pKa = 10.85 GPSVLIRR708 pKa = 11.84 TRR710 pKa = 11.84 AASLTTPP717 pKa = 3.89
Molecular weight: 80.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.223
IPC2_protein 7.585
IPC_protein 7.351
Toseland 6.898
ProMoST 8.024
Dawson 8.083
Bjellqvist 8.58
Wikipedia 7.98
Rodwell 8.112
Grimsley 6.927
Solomon 8.112
Lehninger 8.141
Nozaki 8.858
DTASelect 8.229
Thurlkill 8.287
EMBOSS 8.287
Sillero 8.653
Patrickios 4.38
IPC_peptide 8.112
IPC2_peptide 7.644
IPC2.peptide.svr19 7.646
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2889
353
783
577.8
64.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.784 ± 1.347
2.25 ± 0.611
5.365 ± 0.671
7.58 ± 0.624
4.05 ± 0.432
7.823 ± 0.548
1.904 ± 0.253
3.15 ± 0.248
5.85 ± 0.304
7.996 ± 0.874
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.492 ± 0.355
2.873 ± 0.451
7.061 ± 0.764
4.361 ± 0.158
6.3 ± 0.771
7.2 ± 0.57
5.123 ± 0.292
7.13 ± 0.158
1.869 ± 0.186
2.838 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here