Coriobacteriaceae bacterium CHKCI002
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2836 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143XB61|A0A143XB61_9ACTN Aspartokinase OS=Coriobacteriaceae bacterium CHKCI002 OX=1780377 GN=lysC PE=3 SV=1
MM1 pKa = 7.97 RR2 pKa = 11.84 DD3 pKa = 2.8 SFAYY7 pKa = 10.2 VGNEE11 pKa = 4.04 TVGSMVEE18 pKa = 3.86 ARR20 pKa = 11.84 LGALGYY26 pKa = 10.47 VRR28 pKa = 11.84 ASDD31 pKa = 3.38 VGSARR36 pKa = 11.84 TVVTYY41 pKa = 7.55 CTSQTALEE49 pKa = 4.23 DD50 pKa = 3.89 AYY52 pKa = 10.87 FDD54 pKa = 3.98 EE55 pKa = 5.77 GGLVQAAAAGTLLVDD70 pKa = 5.43 LSPSTPGFARR80 pKa = 11.84 EE81 pKa = 4.07 LNAVAAVSDD90 pKa = 4.17 LVAVEE95 pKa = 4.48 APLAVVDD102 pKa = 4.79 PVRR105 pKa = 11.84 ADD107 pKa = 3.25 AFSDD111 pKa = 3.44 KK112 pKa = 11.35 GNLVCFAAGDD122 pKa = 3.92 EE123 pKa = 4.46 DD124 pKa = 4.19 ALAAARR130 pKa = 11.84 PVLEE134 pKa = 4.31 AVAGSVRR141 pKa = 11.84 DD142 pKa = 3.56 MGGAGAGQLARR153 pKa = 11.84 AAWTLQATAQIVSAVEE169 pKa = 3.49 ADD171 pKa = 3.23 ALYY174 pKa = 10.34 RR175 pKa = 11.84 AVRR178 pKa = 11.84 RR179 pKa = 11.84 SSSPFGEE186 pKa = 3.85 EE187 pKa = 3.62 ALRR190 pKa = 11.84 VGAAAPLAEE199 pKa = 4.4 SVLAAVDD206 pKa = 3.37 EE207 pKa = 4.54 GRR209 pKa = 11.84 FDD211 pKa = 3.15 GGYY214 pKa = 7.01 TVEE217 pKa = 4.35 MFMAEE222 pKa = 4.27 LSAALTAADD231 pKa = 4.7 DD232 pKa = 4.32 ADD234 pKa = 5.37 LILPQAEE241 pKa = 4.32 ACLHH245 pKa = 5.78 LLEE248 pKa = 4.91 LLAVIGGSDD257 pKa = 3.54 KK258 pKa = 11.34 APSALALVYY267 pKa = 10.83 GEE269 pKa = 4.55 EE270 pKa = 4.18 AACAEE275 pKa = 4.53 QGLDD279 pKa = 3.05 WTRR282 pKa = 11.84 AEE284 pKa = 4.28 SAFGEE289 pKa = 4.56 GDD291 pKa = 4.25 DD292 pKa = 4.75 EE293 pKa = 4.76 DD294 pKa = 6.61 AEE296 pKa = 4.64 DD297 pKa = 4.47 GCEE300 pKa = 4.06 EE301 pKa = 4.29 GCDD304 pKa = 4.76 ADD306 pKa = 4.68 GCGCGHH312 pKa = 6.85 AHH314 pKa = 6.76 GGYY317 pKa = 9.16 RR318 pKa = 11.84 DD319 pKa = 3.59 YY320 pKa = 11.14 PDD322 pKa = 3.43 YY323 pKa = 10.65 PGYY326 pKa = 10.52 GAYY329 pKa = 9.92 SANN332 pKa = 3.47
Molecular weight: 33.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.923
IPC_protein 3.923
Toseland 3.706
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.202
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.037
Patrickios 1.316
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A143X0U1|A0A143X0U1_9ACTN Thioesterase superfamily protein OS=Coriobacteriaceae bacterium CHKCI002 OX=1780377 GN=BN3658_00646 PE=4 SV=1
MM1 pKa = 7.38 TPASTAWTWWAPTRR15 pKa = 11.84 TARR18 pKa = 11.84 ARR20 pKa = 11.84 LRR22 pKa = 11.84 SRR24 pKa = 11.84 SARR27 pKa = 11.84 GRR29 pKa = 11.84 RR30 pKa = 11.84 SKK32 pKa = 9.64 TATTTRR38 pKa = 11.84 PPCCPASPCRR48 pKa = 11.84 RR49 pKa = 11.84 SSPGATGRR57 pKa = 11.84 RR58 pKa = 11.84 TTRR61 pKa = 11.84 SRR63 pKa = 11.84 SRR65 pKa = 11.84 CTACAPWTRR74 pKa = 11.84 RR75 pKa = 11.84 ARR77 pKa = 11.84 PLRR80 pKa = 11.84 RR81 pKa = 11.84 AHH83 pKa = 6.42 RR84 pKa = 11.84 CRR86 pKa = 11.84 RR87 pKa = 11.84 PARR90 pKa = 11.84 FPSARR95 pKa = 11.84 SMRR98 pKa = 11.84 PRR100 pKa = 11.84 KK101 pKa = 9.7 RR102 pKa = 11.84 PSPRR106 pKa = 11.84 AAPWRR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 SATSRR118 pKa = 11.84 SPSRR122 pKa = 11.84 APTSTACSSRR132 pKa = 11.84 PRR134 pKa = 11.84 TRR136 pKa = 11.84 AATRR140 pKa = 11.84 RR141 pKa = 11.84 AASTTRR147 pKa = 11.84 ARR149 pKa = 11.84 STRR152 pKa = 11.84 SQWPWPPTGRR162 pKa = 11.84 ATSSPRR168 pKa = 11.84 PRR170 pKa = 11.84 LPLSPTTRR178 pKa = 11.84 ASPPKK183 pKa = 10.28 ARR185 pKa = 11.84 AEE187 pKa = 4.21 AGWPRR192 pKa = 11.84 ASATPTRR199 pKa = 11.84 IPRR202 pKa = 11.84 SPPSTARR209 pKa = 11.84 WTTPTRR215 pKa = 11.84 AARR218 pKa = 11.84 GLWKK222 pKa = 7.22 TTCSRR227 pKa = 11.84 SASKK231 pKa = 9.82 PWATMPRR238 pKa = 11.84 ARR240 pKa = 11.84 RR241 pKa = 11.84 APSSRR246 pKa = 11.84 KK247 pKa = 9.06 RR248 pKa = 11.84 ATQAGCSRR256 pKa = 11.84 SGRR259 pKa = 11.84 PRR261 pKa = 11.84 SRR263 pKa = 11.84 TTTIWRR269 pKa = 11.84 ARR271 pKa = 11.84 RR272 pKa = 11.84 SPTRR276 pKa = 11.84 CGRR279 pKa = 11.84 TSTRR283 pKa = 11.84 ALATARR289 pKa = 11.84 TEE291 pKa = 4.1 PRR293 pKa = 11.84 PHH295 pKa = 6.0 LTGG298 pKa = 3.55
Molecular weight: 33.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.412
IPC2_protein 10.891
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.193
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.901
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.156
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2836
0
2836
947897
29
9826
334.2
36.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.807 ± 0.089
1.653 ± 0.025
5.955 ± 0.053
6.675 ± 0.046
3.828 ± 0.031
8.597 ± 0.049
1.721 ± 0.022
4.301 ± 0.042
3.43 ± 0.041
9.26 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.535 ± 0.024
2.583 ± 0.03
4.464 ± 0.031
2.636 ± 0.027
6.725 ± 0.068
5.838 ± 0.043
4.901 ± 0.045
8.224 ± 0.049
1.152 ± 0.019
2.715 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here