Amino acid dipepetide frequency for Helicobacter cholecystus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.861AlaAla: 1.861 ± 0.098
0.821AlaCys: 0.821 ± 0.06
2.45AlaAsp: 2.45 ± 0.087
2.687AlaGlu: 2.687 ± 0.081
3.29AlaPhe: 3.29 ± 0.097
3.9AlaGly: 3.9 ± 0.108
1.313AlaHis: 1.313 ± 0.061
5.775AlaIle: 5.775 ± 0.131
5.969AlaLys: 5.969 ± 0.119
8.58AlaLeu: 8.58 ± 0.158
1.864AlaMet: 1.864 ± 0.081
3.356AlaAsn: 3.356 ± 0.113
1.942AlaPro: 1.942 ± 0.077
3.831AlaGln: 3.831 ± 0.1
2.613AlaArg: 2.613 ± 0.08
4.718AlaSer: 4.718 ± 0.113
3.134AlaThr: 3.134 ± 0.088
2.76AlaVal: 2.76 ± 0.085
0.421AlaTrp: 0.421 ± 0.03
2.833AlaTyr: 2.833 ± 0.091
0.0AlaXaa: 0.0 ± 0.0
Cys
0.927CysAla: 0.927 ± 0.049
0.142CysCys: 0.142 ± 0.02
0.669CysAsp: 0.669 ± 0.041
0.804CysGlu: 0.804 ± 0.042
0.683CysPhe: 0.683 ± 0.043
1.081CysGly: 1.081 ± 0.059
0.255CysHis: 0.255 ± 0.031
1.135CysIle: 1.135 ± 0.056
0.922CysLys: 0.922 ± 0.044
0.989CysLeu: 0.989 ± 0.054
0.27CysMet: 0.27 ± 0.021
0.62CysAsn: 0.62 ± 0.039
0.447CysPro: 0.447 ± 0.032
0.364CysGln: 0.364 ± 0.033
0.251CysArg: 0.251 ± 0.025
0.837CysSer: 0.837 ± 0.051
0.508CysThr: 0.508 ± 0.038
0.837CysVal: 0.837 ± 0.051
0.083CysTrp: 0.083 ± 0.014
0.376CysTyr: 0.376 ± 0.035
0.0CysXaa: 0.0 ± 0.0
Asp
2.93AspAla: 2.93 ± 0.091
0.603AspCys: 0.603 ± 0.039
1.845AspAsp: 1.845 ± 0.073
3.701AspGlu: 3.701 ± 0.115
3.547AspPhe: 3.547 ± 0.094
2.871AspGly: 2.871 ± 0.095
0.773AspHis: 0.773 ± 0.041
4.113AspIle: 4.113 ± 0.096
3.19AspLys: 3.19 ± 0.098
5.002AspLeu: 5.002 ± 0.107
1.086AspMet: 1.086 ± 0.056
1.857AspAsn: 1.857 ± 0.073
1.358AspPro: 1.358 ± 0.061
1.265AspGln: 1.265 ± 0.058
1.639AspArg: 1.639 ± 0.06
2.769AspSer: 2.769 ± 0.102
2.178AspThr: 2.178 ± 0.075
2.509AspVal: 2.509 ± 0.074
0.421AspTrp: 0.421 ± 0.032
1.949AspTyr: 1.949 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
4.236GluAla: 4.236 ± 0.133
0.965GluCys: 0.965 ± 0.056
2.881GluAsp: 2.881 ± 0.088
5.463GluGlu: 5.463 ± 0.156
3.607GluPhe: 3.607 ± 0.103
4.799GluGly: 4.799 ± 0.117
0.991GluHis: 0.991 ± 0.049
7.199GluIle: 7.199 ± 0.141
5.953GluLys: 5.953 ± 0.141
6.499GluLeu: 6.499 ± 0.143
1.819GluMet: 1.819 ± 0.066
3.886GluAsn: 3.886 ± 0.118
1.211GluPro: 1.211 ± 0.056
2.54GluGln: 2.54 ± 0.084
2.76GluArg: 2.76 ± 0.079
3.855GluSer: 3.855 ± 0.097
1.878GluThr: 1.878 ± 0.074
5.023GluVal: 5.023 ± 0.114
0.584GluTrp: 0.584 ± 0.035
2.443GluTyr: 2.443 ± 0.089
0.0GluXaa: 0.0 ± 0.0
Phe
3.491PheAla: 3.491 ± 0.11
0.977PheCys: 0.977 ± 0.051
2.85PheAsp: 2.85 ± 0.072
3.578PheGlu: 3.578 ± 0.082
3.425PhePhe: 3.425 ± 0.138
4.025PheGly: 4.025 ± 0.11
0.762PheHis: 0.762 ± 0.05
4.087PheIle: 4.087 ± 0.125
3.491PheLys: 3.491 ± 0.088
5.922PheLeu: 5.922 ± 0.165
1.031PheMet: 1.031 ± 0.049
2.448PheAsn: 2.448 ± 0.097
1.511PhePro: 1.511 ± 0.064
1.391PheGln: 1.391 ± 0.067
1.329PheArg: 1.329 ± 0.059
4.463PheSer: 4.463 ± 0.115
2.237PheThr: 2.237 ± 0.07
2.944PheVal: 2.944 ± 0.106
0.558PheTrp: 0.558 ± 0.041
2.244PheTyr: 2.244 ± 0.075
0.0PheXaa: 0.0 ± 0.0
Gly
4.439GlyAla: 4.439 ± 0.117
0.811GlyCys: 0.811 ± 0.047
3.19GlyAsp: 3.19 ± 0.085
5.257GlyGlu: 5.257 ± 0.129
3.647GlyPhe: 3.647 ± 0.101
5.596GlyGly: 5.596 ± 0.169
0.981GlyHis: 0.981 ± 0.053
6.435GlyIle: 6.435 ± 0.128
5.113GlyLys: 5.113 ± 0.113
5.536GlyLeu: 5.536 ± 0.126
1.771GlyMet: 1.771 ± 0.069
3.311GlyAsn: 3.311 ± 0.168
0.769GlyPro: 0.769 ± 0.047
1.528GlyGln: 1.528 ± 0.067
2.382GlyArg: 2.382 ± 0.09
4.605GlySer: 4.605 ± 0.134
2.495GlyThr: 2.495 ± 0.109
5.473GlyVal: 5.473 ± 0.121
0.497GlyTrp: 0.497 ± 0.041
2.84GlyTyr: 2.84 ± 0.08
0.0GlyXaa: 0.0 ± 0.0
His
0.948HisAla: 0.948 ± 0.05
0.296HisCys: 0.296 ± 0.028
0.534HisAsp: 0.534 ± 0.036
0.655HisGlu: 0.655 ± 0.037
1.213HisPhe: 1.213 ± 0.051
0.896HisGly: 0.896 ± 0.045
0.622HisHis: 0.622 ± 0.045
1.767HisIle: 1.767 ± 0.066
1.279HisLys: 1.279 ± 0.054
2.027HisLeu: 2.027 ± 0.064
0.291HisMet: 0.291 ± 0.028
0.903HisAsn: 0.903 ± 0.049
1.024HisPro: 1.024 ± 0.064
0.948HisGln: 0.948 ± 0.05
0.698HisArg: 0.698 ± 0.043
1.741HisSer: 1.741 ± 0.063
1.149HisThr: 1.149 ± 0.05
0.414HisVal: 0.414 ± 0.034
0.154HisTrp: 0.154 ± 0.021
0.899HisTyr: 0.899 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
7.268IleAla: 7.268 ± 0.153
1.156IleCys: 1.156 ± 0.06
4.456IleAsp: 4.456 ± 0.1
5.858IleGlu: 5.858 ± 0.125
4.619IlePhe: 4.619 ± 0.142
5.529IleGly: 5.529 ± 0.129
1.485IleHis: 1.485 ± 0.06
6.244IleIle: 6.244 ± 0.146
6.892IleLys: 6.892 ± 0.117
9.621IleLeu: 9.621 ± 0.206
1.398IleMet: 1.398 ± 0.063
4.006IleAsn: 4.006 ± 0.124
3.42IlePro: 3.42 ± 0.102
3.467IleGln: 3.467 ± 0.112
2.564IleArg: 2.564 ± 0.081
6.13IleSer: 6.13 ± 0.126
4.633IleThr: 4.633 ± 0.149
4.765IleVal: 4.765 ± 0.14
0.575IleTrp: 0.575 ± 0.042
3.112IleTyr: 3.112 ± 0.092
0.0IleXaa: 0.0 ± 0.0
Lys
4.898LysAla: 4.898 ± 0.122
0.57LysCys: 0.57 ± 0.041
4.406LysAsp: 4.406 ± 0.115
7.717LysGlu: 7.717 ± 0.163
2.739LysPhe: 2.739 ± 0.079
4.543LysGly: 4.543 ± 0.104
1.258LysHis: 1.258 ± 0.055
8.107LysIle: 8.107 ± 0.148
6.636LysLys: 6.636 ± 0.161
6.442LysLeu: 6.442 ± 0.144
2.093LysMet: 2.093 ± 0.075
5.22LysAsn: 5.22 ± 0.105
2.162LysPro: 2.162 ± 0.07
2.632LysGln: 2.632 ± 0.084
3.089LysArg: 3.089 ± 0.089
5.153LysSer: 5.153 ± 0.133
3.872LysThr: 3.872 ± 0.093
4.214LysVal: 4.214 ± 0.098
0.473LysTrp: 0.473 ± 0.033
2.493LysTyr: 2.493 ± 0.083
0.0LysXaa: 0.0 ± 0.0
Leu
6.927LeuAla: 6.927 ± 0.156
1.594LeuCys: 1.594 ± 0.07
5.047LeuAsp: 5.047 ± 0.116
7.542LeuGlu: 7.542 ± 0.149
5.262LeuPhe: 5.262 ± 0.158
7.088LeuGly: 7.088 ± 0.143
1.913LeuHis: 1.913 ± 0.063
7.606LeuIle: 7.606 ± 0.171
9.115LeuLys: 9.115 ± 0.154
10.803LeuLeu: 10.803 ± 0.231
2.072LeuMet: 2.072 ± 0.078
5.444LeuAsn: 5.444 ± 0.135
3.786LeuPro: 3.786 ± 0.097
4.659LeuGln: 4.659 ± 0.12
3.952LeuArg: 3.952 ± 0.108
9.259LeuSer: 9.259 ± 0.195
4.387LeuThr: 4.387 ± 0.118
4.56LeuVal: 4.56 ± 0.117
0.769LeuTrp: 0.769 ± 0.049
3.89LeuTyr: 3.89 ± 0.117
0.0LeuXaa: 0.0 ± 0.0
Met
1.45MetAla: 1.45 ± 0.068
0.215MetCys: 0.215 ± 0.023
0.972MetAsp: 0.972 ± 0.05
1.152MetGlu: 1.152 ± 0.048
0.806MetPhe: 0.806 ± 0.05
1.864MetGly: 1.864 ± 0.071
0.435MetHis: 0.435 ± 0.032
2.216MetIle: 2.216 ± 0.073
1.802MetLys: 1.802 ± 0.06
2.417MetLeu: 2.417 ± 0.084
0.679MetMet: 0.679 ± 0.04
1.022MetAsn: 1.022 ± 0.046
0.929MetPro: 0.929 ± 0.046
1.346MetGln: 1.346 ± 0.05
1.062MetArg: 1.062 ± 0.048
1.655MetSer: 1.655 ± 0.067
0.816MetThr: 0.816 ± 0.044
1.119MetVal: 1.119 ± 0.056
0.137MetTrp: 0.137 ± 0.021
0.485MetTyr: 0.485 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
3.985AsnAla: 3.985 ± 0.115
0.35AsnCys: 0.35 ± 0.031
2.02AsnAsp: 2.02 ± 0.068
2.878AsnGlu: 2.878 ± 0.078
2.857AsnPhe: 2.857 ± 0.096
3.417AsnGly: 3.417 ± 0.138
1.055AsnHis: 1.055 ± 0.051
4.794AsnIle: 4.794 ± 0.137
3.25AsnLys: 3.25 ± 0.092
5.82AsnLeu: 5.82 ± 0.135
0.937AsnMet: 0.937 ± 0.04
2.514AsnAsn: 2.514 ± 0.134
2.677AsnPro: 2.677 ± 0.075
2.221AsnGln: 2.221 ± 0.078
1.561AsnArg: 1.561 ± 0.064
3.488AsnSer: 3.488 ± 0.142
2.937AsnThr: 2.937 ± 0.131
2.46AsnVal: 2.46 ± 0.072
0.277AsnTrp: 0.277 ± 0.026
1.831AsnTyr: 1.831 ± 0.068
0.0AsnXaa: 0.0 ± 0.0
Pro
1.542ProAla: 1.542 ± 0.061
0.333ProCys: 0.333 ± 0.029
1.071ProAsp: 1.071 ± 0.05
1.492ProGlu: 1.492 ± 0.064
1.797ProPhe: 1.797 ± 0.065
0.856ProGly: 0.856 ± 0.046
0.927ProHis: 0.927 ± 0.053
2.935ProIle: 2.935 ± 0.093
2.907ProLys: 2.907 ± 0.095
3.921ProLeu: 3.921 ± 0.121
0.657ProMet: 0.657 ± 0.039
1.771ProAsn: 1.771 ± 0.067
1.088ProPro: 1.088 ± 0.063
2.01ProGln: 2.01 ± 0.078
1.012ProArg: 1.012 ± 0.052
2.736ProSer: 2.736 ± 0.086
1.998ProThr: 1.998 ± 0.077
1.476ProVal: 1.476 ± 0.073
0.246ProTrp: 0.246 ± 0.027
1.594ProTyr: 1.594 ± 0.065
0.0ProXaa: 0.0 ± 0.0
Gln
2.878GlnAla: 2.878 ± 0.084
0.364GlnCys: 0.364 ± 0.029
2.053GlnAsp: 2.053 ± 0.071
4.458GlnGlu: 4.458 ± 0.123
1.015GlnPhe: 1.015 ± 0.044
2.33GlnGly: 2.33 ± 0.086
0.471GlnHis: 0.471 ± 0.035
3.772GlnIle: 3.772 ± 0.104
4.458GlnLys: 4.458 ± 0.118
2.017GlnLeu: 2.017 ± 0.071
0.915GlnMet: 0.915 ± 0.042
3.053GlnAsn: 3.053 ± 0.11
0.792GlnPro: 0.792 ± 0.046
0.993GlnGln: 0.993 ± 0.051
1.672GlnArg: 1.672 ± 0.075
3.309GlnSer: 3.309 ± 0.085
2.415GlnThr: 2.415 ± 0.084
1.871GlnVal: 1.871 ± 0.067
0.322GlnTrp: 0.322 ± 0.026
1.123GlnTyr: 1.123 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
2.339ArgAla: 2.339 ± 0.085
0.333ArgCys: 0.333 ± 0.028
1.842ArgAsp: 1.842 ± 0.068
3.259ArgGlu: 3.259 ± 0.109
2.039ArgPhe: 2.039 ± 0.071
2.71ArgGly: 2.71 ± 0.094
0.546ArgHis: 0.546 ± 0.039
3.19ArgIle: 3.19 ± 0.098
2.322ArgLys: 2.322 ± 0.076
3.356ArgLeu: 3.356 ± 0.101
0.906ArgMet: 0.906 ± 0.045
1.741ArgAsn: 1.741 ± 0.066
0.903ArgPro: 0.903 ± 0.048
0.955ArgGln: 0.955 ± 0.049
1.379ArgArg: 1.379 ± 0.067
2.117ArgSer: 2.117 ± 0.08
1.135ArgThr: 1.135 ± 0.05
2.635ArgVal: 2.635 ± 0.099
0.27ArgTrp: 0.27 ± 0.025
1.54ArgTyr: 1.54 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
4.825SerAla: 4.825 ± 0.115
0.738SerCys: 0.738 ± 0.047
2.798SerAsp: 2.798 ± 0.083
3.876SerGlu: 3.876 ± 0.103
4.174SerPhe: 4.174 ± 0.119
4.966SerGly: 4.966 ± 0.144
1.533SerHis: 1.533 ± 0.069
6.104SerIle: 6.104 ± 0.127
5.501SerLys: 5.501 ± 0.111
8.921SerLeu: 8.921 ± 0.208
1.573SerMet: 1.573 ± 0.057
3.633SerAsn: 3.633 ± 0.143
2.557SerPro: 2.557 ± 0.078
3.11SerGln: 3.11 ± 0.089
2.218SerArg: 2.218 ± 0.082
5.851SerSer: 5.851 ± 0.209
3.495SerThr: 3.495 ± 0.128
3.921SerVal: 3.921 ± 0.102
0.464SerTrp: 0.464 ± 0.034
2.94SerTyr: 2.94 ± 0.079
0.0SerXaa: 0.0 ± 0.0
Thr
2.211ThrAla: 2.211 ± 0.087
0.397ThrCys: 0.397 ± 0.034
1.674ThrAsp: 1.674 ± 0.077
1.528ThrGlu: 1.528 ± 0.069
2.445ThrPhe: 2.445 ± 0.089
2.431ThrGly: 2.431 ± 0.118
1.327ThrHis: 1.327 ± 0.059
3.564ThrIle: 3.564 ± 0.119
2.935ThrLys: 2.935 ± 0.087
7.251ThrLeu: 7.251 ± 0.198
0.906ThrMet: 0.906 ± 0.049
2.202ThrAsn: 2.202 ± 0.113
2.748ThrPro: 2.748 ± 0.09
3.221ThrGln: 3.221 ± 0.091
1.454ThrArg: 1.454 ± 0.054
3.663ThrSer: 3.663 ± 0.123
2.457ThrThr: 2.457 ± 0.136
1.14ThrVal: 1.14 ± 0.058
0.315ThrTrp: 0.315 ± 0.031
1.778ThrTyr: 1.778 ± 0.076
0.0ThrXaa: 0.0 ± 0.0
Val
3.663ValAla: 3.663 ± 0.109
0.88ValCys: 0.88 ± 0.052
2.632ValAsp: 2.632 ± 0.089
3.555ValGlu: 3.555 ± 0.109
3.006ValPhe: 3.006 ± 0.095
4.392ValGly: 4.392 ± 0.13
0.84ValHis: 0.84 ± 0.044
4.555ValIle: 4.555 ± 0.102
3.68ValLys: 3.68 ± 0.106
5.856ValLeu: 5.856 ± 0.119
1.48ValMet: 1.48 ± 0.048
2.202ValAsn: 2.202 ± 0.076
1.485ValPro: 1.485 ± 0.069
1.951ValGln: 1.951 ± 0.074
2.058ValArg: 2.058 ± 0.081
3.827ValSer: 3.827 ± 0.096
1.835ValThr: 1.835 ± 0.076
3.484ValVal: 3.484 ± 0.107
0.482ValTrp: 0.482 ± 0.033
1.861ValTyr: 1.861 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.487TrpAla: 0.487 ± 0.035
0.123TrpCys: 0.123 ± 0.018
0.388TrpAsp: 0.388 ± 0.027
0.48TrpGlu: 0.48 ± 0.033
0.352TrpPhe: 0.352 ± 0.031
0.65TrpGly: 0.65 ± 0.047
0.17TrpHis: 0.17 ± 0.018
0.705TrpIle: 0.705 ± 0.044
0.513TrpLys: 0.513 ± 0.04
0.849TrpLeu: 0.849 ± 0.05
0.166TrpMet: 0.166 ± 0.021
0.357TrpAsn: 0.357 ± 0.03
0.099TrpPro: 0.099 ± 0.016
0.274TrpGln: 0.274 ± 0.025
0.291TrpArg: 0.291 ± 0.029
0.419TrpSer: 0.419 ± 0.029
0.22TrpThr: 0.22 ± 0.02
0.516TrpVal: 0.516 ± 0.036
0.097TrpTrp: 0.097 ± 0.019
0.267TrpTyr: 0.267 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.812TyrAla: 2.812 ± 0.093
0.532TyrCys: 0.532 ± 0.029
1.828TyrAsp: 1.828 ± 0.065
2.649TyrGlu: 2.649 ± 0.084
2.24TyrPhe: 2.24 ± 0.068
2.732TyrGly: 2.732 ± 0.091
0.802TyrHis: 0.802 ± 0.043
2.895TyrIle: 2.895 ± 0.091
2.788TyrLys: 2.788 ± 0.077
3.845TyrLeu: 3.845 ± 0.11
0.665TyrMet: 0.665 ± 0.037
1.783TyrAsn: 1.783 ± 0.072
1.464TyrPro: 1.464 ± 0.061
1.575TyrGln: 1.575 ± 0.059
1.535TyrArg: 1.535 ± 0.064
2.601TyrSer: 2.601 ± 0.087
1.868TyrThr: 1.868 ± 0.066
1.603TyrVal: 1.603 ± 0.059
0.281TyrTrp: 0.281 ± 0.028
1.428TyrTyr: 1.428 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1316 proteins (422834 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski