Air potato virus 1
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G6V9C3|A0A3G6V9C3_9CLOS ORF5 OS=Air potato virus 1 OX=2491018 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.49 SHH4 pKa = 6.95 LEE6 pKa = 4.07 VVNVMFDD13 pKa = 3.45 HH14 pKa = 5.76 YY15 pKa = 10.68 QINGLPFSMKK25 pKa = 9.17 WVEE28 pKa = 4.91 DD29 pKa = 3.58 LFQFSDD35 pKa = 3.93 FSTVVDD41 pKa = 3.44 NVTITEE47 pKa = 4.18 CQKK50 pKa = 9.6 TPKK53 pKa = 10.07 AYY55 pKa = 9.58 KK56 pKa = 9.89 IKK58 pKa = 10.93 SLVPLVNSLRR68 pKa = 11.84 PSPRR72 pKa = 11.84 PQSLISNLYY81 pKa = 9.09 VFEE84 pKa = 4.13 TRR86 pKa = 11.84 NYY88 pKa = 8.56 NADD91 pKa = 3.54 RR92 pKa = 11.84 GVQNYY97 pKa = 7.49 YY98 pKa = 10.16 RR99 pKa = 11.84 PPVVKK104 pKa = 10.21 EE105 pKa = 3.72 LVDD108 pKa = 3.84 TFFSTYY114 pKa = 9.23 IDD116 pKa = 3.6 AGRR119 pKa = 11.84 MCEE122 pKa = 4.09 MIGDD126 pKa = 3.64 IRR128 pKa = 11.84 VVNSVSLEE136 pKa = 3.73 EE137 pKa = 4.35 WLDD140 pKa = 3.31 SRR142 pKa = 11.84 PAMGKK147 pKa = 9.89 SGLLNDD153 pKa = 4.09 LTKK156 pKa = 10.95 DD157 pKa = 3.28 PYY159 pKa = 11.16 YY160 pKa = 10.51 QDD162 pKa = 3.44 SLNHH166 pKa = 6.39 FKK168 pKa = 11.46 YY169 pKa = 9.68 MIKK172 pKa = 10.29 SDD174 pKa = 4.14 LKK176 pKa = 11.24 SKK178 pKa = 10.8 LDD180 pKa = 3.35 YY181 pKa = 9.61 TARR184 pKa = 11.84 EE185 pKa = 4.04 NLASGQNIVYY195 pKa = 9.92 HH196 pKa = 5.68 EE197 pKa = 4.15 RR198 pKa = 11.84 RR199 pKa = 11.84 ICALFSSVFQDD210 pKa = 3.36 LVRR213 pKa = 11.84 VLKK216 pKa = 10.49 FVLADD221 pKa = 3.54 KK222 pKa = 10.58 FLLYY226 pKa = 10.61 HH227 pKa = 6.47 GVNLDD232 pKa = 3.55 EE233 pKa = 5.18 FAQAMTNKK241 pKa = 7.72 MTFPLASCYY250 pKa = 10.25 CGEE253 pKa = 4.12 VDD255 pKa = 3.66 ISKK258 pKa = 10.61 YY259 pKa = 10.67 DD260 pKa = 3.33 KK261 pKa = 10.96 SQNQMTKK268 pKa = 9.12 EE269 pKa = 4.04 VEE271 pKa = 3.81 LEE273 pKa = 3.57 IYY275 pKa = 10.02 RR276 pKa = 11.84 RR277 pKa = 11.84 LGMHH281 pKa = 7.48 PDD283 pKa = 4.37 LVDD286 pKa = 3.95 LWACSDD292 pKa = 3.75 FSSRR296 pKa = 11.84 VGSMKK301 pKa = 10.5 EE302 pKa = 4.18 GILLDD307 pKa = 3.24 IGAQRR312 pKa = 11.84 RR313 pKa = 11.84 TGTATTWIGNTLINMTLLAAAGDD336 pKa = 3.86 VKK338 pKa = 11.21 NFDD341 pKa = 3.31 IAAFSGDD348 pKa = 3.78 DD349 pKa = 3.39 SLLLSRR355 pKa = 11.84 EE356 pKa = 4.04 RR357 pKa = 11.84 IEE359 pKa = 4.94 LDD361 pKa = 3.24 SFVYY365 pKa = 9.92 EE366 pKa = 3.75 YY367 pKa = 11.27 HH368 pKa = 6.71 FGFDD372 pKa = 3.26 VKK374 pKa = 10.53 FYY376 pKa = 10.98 GQATPYY382 pKa = 9.71 FCSKK386 pKa = 10.53 FLIPVNGRR394 pKa = 11.84 VVFVPDD400 pKa = 3.59 ALKK403 pKa = 11.15 LFVNLGAEE411 pKa = 4.09 WDD413 pKa = 3.75 CDD415 pKa = 3.77 KK416 pKa = 11.94 NEE418 pKa = 3.96 MFEE421 pKa = 4.18 KK422 pKa = 10.59 FKK424 pKa = 11.44 SFVDD428 pKa = 3.86 LTKK431 pKa = 11.07 DD432 pKa = 3.26 LVDD435 pKa = 3.55 NSVVEE440 pKa = 3.84 ILAVYY445 pKa = 10.2 IEE447 pKa = 4.53 EE448 pKa = 4.9 KK449 pKa = 10.57 YY450 pKa = 11.09 GSNPWVFPSLCCINSLRR467 pKa = 11.84 SNFAQFMRR475 pKa = 11.84 CWRR478 pKa = 11.84 LCQKK482 pKa = 10.81 LLLKK486 pKa = 10.59 DD487 pKa = 3.89 RR488 pKa = 11.84 EE489 pKa = 4.34 LGDD492 pKa = 3.46 VAEE495 pKa = 5.88 DD496 pKa = 3.43 IDD498 pKa = 4.26 EE499 pKa = 4.66 GGG501 pKa = 3.31
Molecular weight: 57.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.057
IPC2_protein 5.156
IPC_protein 5.118
Toseland 5.041
ProMoST 5.27
Dawson 5.13
Bjellqvist 5.258
Wikipedia 5.016
Rodwell 5.029
Grimsley 4.978
Solomon 5.13
Lehninger 5.08
Nozaki 5.245
DTASelect 5.448
Thurlkill 5.067
EMBOSS 5.067
Sillero 5.308
Patrickios 3.884
IPC_peptide 5.13
IPC2_peptide 5.308
IPC2.peptide.svr19 5.265
Protein with the highest isoelectric point:
>tr|A0A3G6V9B9|A0A3G6V9B9_9CLOS ORF3 OS=Air potato virus 1 OX=2491018 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.42 VDD4 pKa = 4.25 DD5 pKa = 4.39 SVNTGVSFSIIVFTFFLLIVIAATVVFCLYY35 pKa = 10.78 FLFTKK40 pKa = 10.46 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 EE44 pKa = 3.71 QSGVEE49 pKa = 3.76 RR50 pKa = 11.84 PTFF53 pKa = 3.54
Molecular weight: 6.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.714
IPC2_protein 8.331
IPC_protein 8.799
Toseland 9.077
ProMoST 8.785
Dawson 9.194
Bjellqvist 9.18
Wikipedia 9.253
Rodwell 9.238
Grimsley 8.829
Solomon 9.648
Lehninger 9.648
Nozaki 9.399
DTASelect 9.048
Thurlkill 9.136
EMBOSS 9.399
Sillero 9.37
Patrickios 7.629
IPC_peptide 9.633
IPC2_peptide 8.287
IPC2.peptide.svr19 7.792
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4305
53
2191
615.0
68.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.179 ± 0.3
1.928 ± 0.13
4.785 ± 0.596
5.807 ± 0.076
4.599 ± 0.455
6.179 ± 0.318
2.23 ± 0.312
5.203 ± 0.399
5.947 ± 0.59
9.895 ± 0.337
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.974 ± 0.152
4.019 ± 0.259
5.134 ± 0.402
3.089 ± 0.23
5.645 ± 0.265
8.548 ± 0.514
6.039 ± 0.41
8.293 ± 0.726
1.092 ± 0.171
3.415 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here