Gordonia Terrae phage RoadKill

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6ENM2|A0A4Y6ENM2_9CAUD Uncharacterized protein OS=Gordonia Terrae phage RoadKill OX=2588506 GN=42 PE=4 SV=1
MM1 pKa = 7.54TSPPGGGTPIVPDD14 pKa = 3.62KK15 pKa = 11.06GYY17 pKa = 8.24TAQTIYY23 pKa = 10.74QLQNVDD29 pKa = 3.41PDD31 pKa = 3.97ALGVEE36 pKa = 4.56ANLDD40 pKa = 3.41LWEE43 pKa = 4.1MLEE46 pKa = 3.95NFRR49 pKa = 11.84NKK51 pKa = 10.5LLTDD55 pKa = 4.12LLGGFASVPIAILDD69 pKa = 4.5GIGDD73 pKa = 4.34VISAITGILNGDD85 pKa = 3.68FDD87 pKa = 6.02DD88 pKa = 4.15LHH90 pKa = 6.7EE91 pKa = 4.28WAQNVIDD98 pKa = 4.6GFGNLLDD105 pKa = 4.57ALQGNYY111 pKa = 9.47TGTDD115 pKa = 3.15SFLLQIQTWAGDD127 pKa = 3.59LFAWVQDD134 pKa = 3.58VADD137 pKa = 4.9FVQNLLDD144 pKa = 4.95AILRR148 pKa = 11.84GIRR151 pKa = 11.84GIPVVGGAIADD162 pKa = 4.14VISDD166 pKa = 3.73LTGLNTKK173 pKa = 8.22ATNALSTANSAAADD187 pKa = 3.42AAAAQVAINDD197 pKa = 4.34LSADD201 pKa = 3.54MAALQVTQVYY211 pKa = 10.05EE212 pKa = 4.17SLSQHH217 pKa = 6.94DD218 pKa = 3.7IVSFPRR224 pKa = 11.84YY225 pKa = 7.89TLGLGADD232 pKa = 4.09GTTGSAGSMSCADD245 pKa = 3.82ASHH248 pKa = 5.92SHH250 pKa = 6.41SGHH253 pKa = 5.12SHH255 pKa = 5.24SVGHH259 pKa = 6.13EE260 pKa = 3.92MPTANPGKK268 pKa = 9.05GTVGYY273 pKa = 10.74VPLFVNRR280 pKa = 11.84FCRR283 pKa = 11.84PRR285 pKa = 11.84RR286 pKa = 11.84LKK288 pKa = 10.88LITGSTGWSIFTIDD302 pKa = 3.32YY303 pKa = 9.1WYY305 pKa = 11.17VALCVYY311 pKa = 10.34NPSTGNVEE319 pKa = 4.25KK320 pKa = 10.9VWDD323 pKa = 4.22GGDD326 pKa = 3.2QKK328 pKa = 11.65GAITTAAKK336 pKa = 10.29LHH338 pKa = 6.07AFDD341 pKa = 4.59MGVLNDD347 pKa = 3.78VTPGTILFGAQLQNAGLIVNTRR369 pKa = 11.84PIAALWQPGLQDD381 pKa = 3.77PSAEE385 pKa = 4.16LLTAPFYY392 pKa = 10.92QLTGQSSIPSSVPLSSLSANNDD414 pKa = 3.42TLPWMGVGVDD424 pKa = 3.85PVV426 pKa = 3.09

Molecular weight: 44.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EUK6|A0A4Y6EUK6_9CAUD Uncharacterized protein OS=Gordonia Terrae phage RoadKill OX=2588506 GN=14 PE=4 SV=1
MM1 pKa = 7.12MGGYY5 pKa = 8.27QPRR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 6.37ADD12 pKa = 2.82AGRR15 pKa = 11.84PFPYY19 pKa = 10.36DD20 pKa = 3.13QVRR23 pKa = 11.84PPAGGGGVYY32 pKa = 10.25RR33 pKa = 11.84SGPGKK38 pKa = 10.01VRR40 pKa = 11.84RR41 pKa = 11.84EE42 pKa = 4.24FIDD45 pKa = 3.58RR46 pKa = 11.84AALAAAAEE54 pKa = 4.12AARR57 pKa = 11.84IEE59 pKa = 4.25RR60 pKa = 11.84RR61 pKa = 11.84ARR63 pKa = 11.84DD64 pKa = 3.35QEE66 pKa = 4.03RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84STARR73 pKa = 11.84LEE75 pKa = 4.24QIAYY79 pKa = 7.61PAGVPKK85 pKa = 10.46RR86 pKa = 11.84APEE89 pKa = 3.99LVDD92 pKa = 4.09LGSNEE97 pKa = 3.97WVGGKK102 pKa = 9.24PPRR105 pKa = 11.84TGPNVFEE112 pKa = 4.69RR113 pKa = 11.84AFSSIRR119 pKa = 11.84EE120 pKa = 4.32HH121 pKa = 4.85VTAWYY126 pKa = 8.81CALAFTIIMIAGSAAGWLVLLVCLASVSAVAAGIALVIGRR166 pKa = 11.84RR167 pKa = 11.84QYY169 pKa = 10.59RR170 pKa = 11.84RR171 pKa = 11.84KK172 pKa = 9.93LAAEE176 pKa = 4.07HH177 pKa = 6.14AAIAARR183 pKa = 11.84ADD185 pKa = 3.96EE186 pKa = 4.38QHH188 pKa = 6.35HH189 pKa = 5.44QWMTDD194 pKa = 2.79PDD196 pKa = 2.99AWRR199 pKa = 11.84RR200 pKa = 11.84QIEE203 pKa = 4.5GTDD206 pKa = 3.35EE207 pKa = 3.87RR208 pKa = 5.21

Molecular weight: 22.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

18004

47

1708

214.3

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.636 ± 0.437

0.794 ± 0.137

7.504 ± 0.522

5.615 ± 0.526

2.505 ± 0.213

8.837 ± 0.53

1.883 ± 0.205

4.471 ± 0.229

2.916 ± 0.211

7.804 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.117

2.716 ± 0.201

5.86 ± 0.256

3.788 ± 0.184

6.982 ± 0.575

4.921 ± 0.329

7.265 ± 0.388

7.226 ± 0.233

2.111 ± 0.148

2.261 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski