Capybara microvirus Cap3_SP_442
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W550|A0A4P8W550_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_442 OX=2585454 PE=3 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 DD3 pKa = 3.45 VVKK6 pKa = 10.78 KK7 pKa = 10.0 MCDD10 pKa = 2.95 EE11 pKa = 4.17 LTAHH15 pKa = 6.39 GVVFSLSEE23 pKa = 4.19 VKK25 pKa = 10.56 KK26 pKa = 10.04 LTDD29 pKa = 3.37 AQVNCVLEE37 pKa = 4.59 MIASCKK43 pKa = 9.27 EE44 pKa = 3.69 PSVIASFIATASRR57 pKa = 11.84 CSVNISSEE65 pKa = 4.14 DD66 pKa = 3.49 VAKK69 pKa = 11.03 QNDD72 pKa = 3.56 DD73 pKa = 3.29 HH74 pKa = 7.56 QVYY77 pKa = 10.45 VDD79 pKa = 3.68 EE80 pKa = 6.17 DD81 pKa = 3.88 KK82 pKa = 11.4 LKK84 pKa = 11.05 SDD86 pKa = 3.93 ANVVGLFGVFDD97 pKa = 3.88 RR98 pKa = 11.84 QSNFIRR104 pKa = 11.84 SWFFCDD110 pKa = 4.02 NEE112 pKa = 4.14 VQARR116 pKa = 11.84 RR117 pKa = 11.84 FFKK120 pKa = 10.94 LSASKK125 pKa = 10.84 LDD127 pKa = 3.54 ADD129 pKa = 3.92 IVLSPEE135 pKa = 4.48 DD136 pKa = 3.72 FDD138 pKa = 5.74 LVLLAALGRR147 pKa = 11.84 SGLVQPLASRR157 pKa = 11.84 VVFNFAEE164 pKa = 4.36 LFDD167 pKa = 4.19 KK168 pKa = 11.09 QLDD171 pKa = 3.86 KK172 pKa = 11.65
Molecular weight: 19.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.919
IPC2_protein 4.914
IPC_protein 4.813
Toseland 4.66
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.698
Rodwell 4.673
Grimsley 4.571
Solomon 4.787
Lehninger 4.749
Nozaki 4.902
DTASelect 5.13
Thurlkill 4.685
EMBOSS 4.724
Sillero 4.952
Patrickios 4.291
IPC_peptide 4.8
IPC2_peptide 4.94
IPC2.peptide.svr19 4.974
Protein with the highest isoelectric point:
>tr|A0A4P8W4U1|A0A4P8W4U1_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_442 OX=2585454 PE=4 SV=1
MM1 pKa = 7.41 SLLAAAAGNIFDD13 pKa = 3.98 RR14 pKa = 11.84 ALNIGEE20 pKa = 4.33 GAATSAINSAIGWKK34 pKa = 7.83 YY35 pKa = 9.34 QKK37 pKa = 11.02 KK38 pKa = 9.98 AMNYY42 pKa = 9.08 SDD44 pKa = 3.29 QLQRR48 pKa = 11.84 SFAHH52 pKa = 6.83 DD53 pKa = 3.12 SYY55 pKa = 11.83 GIMRR59 pKa = 11.84 SGLEE63 pKa = 3.85 DD64 pKa = 3.16 AGYY67 pKa = 11.0 NPLLSLSGSSNSEE80 pKa = 3.8 PLVAGASSASGTGSGQGMGNAAAMKK105 pKa = 8.83 QAKK108 pKa = 9.39 AAEE111 pKa = 4.22 EE112 pKa = 4.23 LKK114 pKa = 10.91 DD115 pKa = 3.87 SQIDD119 pKa = 3.75 LNASQEE125 pKa = 4.21 ALNGTNSAKK134 pKa = 10.57 AQADD138 pKa = 3.88 INNSTAMTKK147 pKa = 10.5 AQVDD151 pKa = 3.97 LAGSQSLLNGMNSAKK166 pKa = 10.55 SQVDD170 pKa = 3.65 MKK172 pKa = 11.25 KK173 pKa = 10.56 LLADD177 pKa = 3.92 IKK179 pKa = 11.17 NNTAITKK186 pKa = 9.81 AQVGLINAQAKK197 pKa = 8.14 AARR200 pKa = 11.84 YY201 pKa = 8.49 GLAGNEE207 pKa = 3.79 YY208 pKa = 10.26 EE209 pKa = 4.35 AMKK212 pKa = 10.81 NKK214 pKa = 10.3 LYY216 pKa = 9.93 STLAKK221 pKa = 10.19 EE222 pKa = 4.28 NPNAFKK228 pKa = 11.17 AMLVGQGATSTAKK241 pKa = 10.94 GIGEE245 pKa = 4.32 ALRR248 pKa = 11.84 SFLPKK253 pKa = 10.37 FSVSFGKK260 pKa = 10.44
Molecular weight: 26.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.791
IPC2_protein 8.726
IPC_protein 8.624
Toseland 9.487
ProMoST 9.238
Dawson 9.721
Bjellqvist 9.341
Wikipedia 9.853
Rodwell 10.16
Grimsley 9.78
Solomon 9.75
Lehninger 9.721
Nozaki 9.443
DTASelect 9.341
Thurlkill 9.56
EMBOSS 9.897
Sillero 9.619
Patrickios 5.499
IPC_peptide 9.75
IPC2_peptide 7.702
IPC2.peptide.svr19 7.902
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1509
153
557
301.8
33.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.687 ± 2.169
1.657 ± 0.454
6.428 ± 0.817
4.771 ± 0.716
5.633 ± 0.683
5.964 ± 1.111
1.657 ± 0.359
4.307 ± 0.397
5.898 ± 1.037
9.079 ± 0.399
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.651 ± 0.355
6.097 ± 0.67
4.307 ± 1.105
4.838 ± 0.594
5.235 ± 0.809
9.079 ± 0.565
3.91 ± 0.54
5.765 ± 1.087
0.795 ± 0.205
4.241 ± 0.822
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here