Caenorhabditis brenneri (Nematode worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0P6K2|G0P6K2_CAEBE CBN-TIN-10 protein OS=Caenorhabditis brenneri OX=135651 GN=Cbn-tin-10 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.14FQLVFLLAALIGSSQAQGLPGVICGITGILCPPTTVAPTTTTTTVPTTTTASDD55 pKa = 3.19TSEE58 pKa = 3.66ICFTIPGNNVNIVIDD73 pKa = 4.05PTQLTLTEE81 pKa = 4.26IGQIKK86 pKa = 9.71QLVEE90 pKa = 4.23DD91 pKa = 4.04SLANILGSLGGTINGALNGLVGGLTGILNPATTPTPTGDD130 pKa = 3.33GQICVVIPNSDD141 pKa = 3.16IDD143 pKa = 3.7EE144 pKa = 4.66LEE146 pKa = 4.21AFLEE150 pKa = 4.6DD151 pKa = 4.02VFSLLTAFFNCLKK164 pKa = 10.66DD165 pKa = 4.13LEE167 pKa = 4.45EE168 pKa = 4.22TTAQFQAEE176 pKa = 4.27VDD178 pKa = 3.87RR179 pKa = 11.84VRR181 pKa = 11.84MEE183 pKa = 3.62IDD185 pKa = 3.07TLVARR190 pKa = 11.84FNNCDD195 pKa = 2.92IVTCYY200 pKa = 10.69LGVANDD206 pKa = 3.45AYY208 pKa = 10.38EE209 pKa = 4.23LVRR212 pKa = 11.84SLPARR217 pKa = 11.84FLTYY221 pKa = 10.59PNTVRR226 pKa = 11.84DD227 pKa = 4.34NINQCVTEE235 pKa = 4.54TINAA239 pKa = 3.89

Molecular weight: 25.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0PHB7|G0PHB7_CAEBE Uncharacterized protein OS=Caenorhabditis brenneri OX=135651 GN=CAEBREN_02941 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.44SNRR5 pKa = 11.84SNSRR9 pKa = 11.84GRR11 pKa = 11.84QRR13 pKa = 11.84DD14 pKa = 3.38SKK16 pKa = 11.55GRR18 pKa = 11.84FTPKK22 pKa = 9.65RR23 pKa = 11.84KK24 pKa = 7.58QTIVEE29 pKa = 4.4RR30 pKa = 11.84SRR32 pKa = 11.84SRR34 pKa = 11.84SRR36 pKa = 11.84SSSRR40 pKa = 11.84GGHH43 pKa = 4.68RR44 pKa = 11.84RR45 pKa = 11.84GRR47 pKa = 11.84SSTRR51 pKa = 11.84KK52 pKa = 8.5SLAVSLHH59 pKa = 5.07TARR62 pKa = 11.84SPSVSSQRR70 pKa = 11.84SSSRR74 pKa = 11.84RR75 pKa = 11.84SVMRR79 pKa = 11.84SSRR82 pKa = 11.84STSSRR87 pKa = 11.84RR88 pKa = 11.84SSSRR92 pKa = 11.84GSVYY96 pKa = 10.76GKK98 pKa = 8.4TGRR101 pKa = 11.84SRR103 pKa = 11.84SSHH106 pKa = 5.67RR107 pKa = 11.84SSRR110 pKa = 11.84SRR112 pKa = 11.84SASRR116 pKa = 11.84SATRR120 pKa = 11.84SSSSRR125 pKa = 11.84SRR127 pKa = 11.84SSSRR131 pKa = 11.84SRR133 pKa = 11.84RR134 pKa = 11.84SASQKK139 pKa = 8.84KK140 pKa = 9.4KK141 pKa = 9.3KK142 pKa = 9.12VVRR145 pKa = 11.84RR146 pKa = 11.84GRR148 pKa = 11.84AARR151 pKa = 11.84SAKK154 pKa = 8.3STKK157 pKa = 8.9SLKK160 pKa = 9.03TSRR163 pKa = 11.84SRR165 pKa = 11.84SRR167 pKa = 11.84SQGPSEE173 pKa = 4.28KK174 pKa = 10.12KK175 pKa = 10.14SRR177 pKa = 11.84TPVARR182 pKa = 11.84STRR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84AA188 pKa = 3.07

Molecular weight: 21.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29979

3

29982

11989625

8

22383

399.9

45.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.231 ± 0.018

1.926 ± 0.012

5.433 ± 0.014

7.157 ± 0.024

4.568 ± 0.014

5.297 ± 0.018

2.303 ± 0.009

5.864 ± 0.012

6.602 ± 0.019

8.483 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.007

4.843 ± 0.01

5.131 ± 0.022

4.166 ± 0.015

5.34 ± 0.014

7.775 ± 0.021

5.817 ± 0.018

6.185 ± 0.012

1.109 ± 0.005

3.098 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski