Lake Sarah-associated circular virus-30

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQN3|A0A140AQN3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-30 OX=1685758 PE=4 SV=1
MM1 pKa = 7.25SQGLYY6 pKa = 9.64WILTIPHH13 pKa = 7.33ADD15 pKa = 3.73FLPYY19 pKa = 10.37LPPGVQYY26 pKa = 10.81IRR28 pKa = 11.84GQLEE32 pKa = 3.71ASTLCRR38 pKa = 11.84PLADD42 pKa = 5.4DD43 pKa = 3.95YY44 pKa = 11.98GADD47 pKa = 3.29AGYY50 pKa = 10.03LHH52 pKa = 6.76WQLLVAFTRR61 pKa = 11.84KK62 pKa = 9.16CRR64 pKa = 11.84LAVVTRR70 pKa = 11.84TFGAGIHH77 pKa = 6.52AEE79 pKa = 4.25LSRR82 pKa = 11.84SSAANDD88 pKa = 3.69YY89 pKa = 9.65VWKK92 pKa = 10.24EE93 pKa = 3.96DD94 pKa = 3.4TRR96 pKa = 11.84VGGTQFEE103 pKa = 4.45LGKK106 pKa = 10.55LAFKK110 pKa = 10.74RR111 pKa = 11.84NSATDD116 pKa = 3.26WSSVRR121 pKa = 11.84DD122 pKa = 3.52SAKK125 pKa = 10.29RR126 pKa = 11.84GRR128 pKa = 11.84LDD130 pKa = 5.83DD131 pKa = 4.29IPSDD135 pKa = 3.24IYY137 pKa = 10.77VRR139 pKa = 11.84CYY141 pKa = 10.73NQLKK145 pKa = 10.45RR146 pKa = 11.84IACDD150 pKa = 3.22HH151 pKa = 5.79MQADD155 pKa = 3.67PMEE158 pKa = 5.46RR159 pKa = 11.84EE160 pKa = 4.56VVCYY164 pKa = 9.33WGRR167 pKa = 11.84SGSGKK172 pKa = 8.71SRR174 pKa = 11.84RR175 pKa = 11.84AWEE178 pKa = 3.89EE179 pKa = 4.03SGVNAYY185 pKa = 9.72PKK187 pKa = 10.39DD188 pKa = 3.75PRR190 pKa = 11.84SKK192 pKa = 10.25FWDD195 pKa = 4.41GYY197 pKa = 9.92RR198 pKa = 11.84GQEE201 pKa = 3.8HH202 pKa = 5.89VVLDD206 pKa = 4.06EE207 pKa = 4.0FRR209 pKa = 11.84GGIDD213 pKa = 2.92IAHH216 pKa = 6.28VLRR219 pKa = 11.84WFDD222 pKa = 3.68RR223 pKa = 11.84YY224 pKa = 9.64PVLEE228 pKa = 4.33GQGEE232 pKa = 4.79LGTLICKK239 pKa = 10.05EE240 pKa = 4.17DD241 pKa = 4.08LDD243 pKa = 4.94HH244 pKa = 6.58EE245 pKa = 4.47QHH247 pKa = 6.85SPP249 pKa = 3.14

Molecular weight: 28.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQN3|A0A140AQN3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-30 OX=1685758 PE=4 SV=1
MM1 pKa = 7.42PAVNGRR7 pKa = 11.84MTRR10 pKa = 11.84DD11 pKa = 2.56TWGDD15 pKa = 3.15HH16 pKa = 4.04FRR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 9.83YY21 pKa = 10.56GAASTMYY28 pKa = 9.63YY29 pKa = 9.93LHH31 pKa = 6.63NRR33 pKa = 11.84LPLSEE38 pKa = 4.06AQQRR42 pKa = 11.84KK43 pKa = 7.31AANWHH48 pKa = 6.09RR49 pKa = 11.84GKK51 pKa = 9.07KK52 pKa = 8.52TSSSQKK58 pKa = 10.27KK59 pKa = 8.33MSNGKK64 pKa = 9.62RR65 pKa = 11.84GRR67 pKa = 11.84SMSRR71 pKa = 11.84GRR73 pKa = 11.84SAKK76 pKa = 9.53RR77 pKa = 11.84RR78 pKa = 11.84PVTPRR83 pKa = 11.84RR84 pKa = 11.84TARR87 pKa = 11.84RR88 pKa = 11.84VSLPATPTARR98 pKa = 11.84SRR100 pKa = 11.84SVSRR104 pKa = 11.84GRR106 pKa = 11.84RR107 pKa = 11.84SARR110 pKa = 11.84SHH112 pKa = 5.13SVRR115 pKa = 11.84RR116 pKa = 11.84MPSLSRR122 pKa = 11.84SRR124 pKa = 11.84SAVSQPSASGSTGPSHH140 pKa = 6.6YY141 pKa = 10.45GSSARR146 pKa = 11.84GAGNLVVGTASSMLKK161 pKa = 10.12KK162 pKa = 8.63YY163 pKa = 10.82AKK165 pKa = 9.76YY166 pKa = 10.41GVVGEE171 pKa = 4.47DD172 pKa = 2.64QWTKK176 pKa = 10.66TIASTAAPIYY186 pKa = 10.21YY187 pKa = 9.98VVGHH191 pKa = 6.62CAPPNTPLLRR201 pKa = 11.84YY202 pKa = 9.15IAAATVKK209 pKa = 10.67AFFLQFGFGFDD220 pKa = 3.14NFTRR224 pKa = 11.84PLSDD228 pKa = 3.41YY229 pKa = 11.14GFTANTDD236 pKa = 3.5IIEE239 pKa = 4.37AQYY242 pKa = 9.43VTTPSGAATSFNIYY256 pKa = 9.63TYY258 pKa = 11.24VVGNTVGDD266 pKa = 3.77VVDD269 pKa = 3.95AFFVAFAGSSKK280 pKa = 10.38NLRR283 pKa = 11.84WDD285 pKa = 3.38FLKK288 pKa = 10.77FKK290 pKa = 10.8SVANPSSVHH299 pKa = 3.56VTMYY303 pKa = 10.4LQNAKK308 pKa = 9.38IHH310 pKa = 6.13LNSTSFLKK318 pKa = 10.67LQNRR322 pKa = 11.84TVEE325 pKa = 4.03SSADD329 pKa = 3.37IEE331 pKa = 4.32TDD333 pKa = 3.5DD334 pKa = 4.37VDD336 pKa = 3.82IMPLIGRR343 pKa = 11.84QFDD346 pKa = 3.55INGTILSLSNDD357 pKa = 3.97GYY359 pKa = 11.41QYY361 pKa = 11.75ACDD364 pKa = 3.42EE365 pKa = 4.39SFAKK369 pKa = 10.64GGLCTKK375 pKa = 10.66DD376 pKa = 2.87NGSAIKK382 pKa = 10.21AIPYY386 pKa = 7.87NAQVRR391 pKa = 11.84GKK393 pKa = 8.56STSKK397 pKa = 10.85NITVAPGEE405 pKa = 4.15ILTSKK410 pKa = 10.37IKK412 pKa = 10.41FEE414 pKa = 3.79KK415 pKa = 9.93TIYY418 pKa = 9.93FSKK421 pKa = 10.05LQSYY425 pKa = 10.1FDD427 pKa = 4.75NINGTGDD434 pKa = 2.86ISEE437 pKa = 4.4RR438 pKa = 11.84TWGKK442 pKa = 10.0SRR444 pKa = 11.84FFFFEE449 pKa = 4.07RR450 pKa = 11.84PIKK453 pKa = 10.78SLGDD457 pKa = 3.22GLAFNFKK464 pKa = 11.01AEE466 pKa = 4.25LDD468 pKa = 3.81VCHH471 pKa = 6.27EE472 pKa = 4.17VMVVGGNPPITTSTYY487 pKa = 10.05TIVAAA492 pKa = 4.79

Molecular weight: 54.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

741

249

492

370.5

41.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.637 ± 0.567

1.35 ± 0.598

5.533 ± 1.409

3.509 ± 1.191

4.588 ± 0.775

8.232 ± 0.34

2.294 ± 0.518

4.453 ± 0.247

5.263 ± 0.703

6.478 ± 1.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.754 ± 0.31

3.779 ± 1.224

4.453 ± 0.02

3.104 ± 0.514

7.962 ± 0.492

9.582 ± 1.779

6.478 ± 1.614

6.343 ± 0.18

1.754 ± 0.822

4.453 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski