Vibrio phage jenny 12G5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9U9C2|R9U9C2_9CAUD Uncharacterized protein OS=Vibrio phage jenny 12G5 OX=573176 GN=VPJG_00028 PE=4 SV=1
MM1 pKa = 7.69IVQDD5 pKa = 4.72PLNPTADD12 pKa = 3.94ADD14 pKa = 4.21SMVSVEE20 pKa = 4.17DD21 pKa = 3.27ARR23 pKa = 11.84LRR25 pKa = 11.84AVSLGVALPADD36 pKa = 3.76DD37 pKa = 4.74TEE39 pKa = 5.14CEE41 pKa = 4.07VALVKK46 pKa = 11.09GNVYY50 pKa = 10.67LNSLCFYY57 pKa = 9.92GEE59 pKa = 4.01PVVPFQQTCFPRR71 pKa = 11.84KK72 pKa = 9.99DD73 pKa = 3.01ISINNNEE80 pKa = 4.36YY81 pKa = 10.18PDD83 pKa = 4.06DD84 pKa = 4.54QIPQQAIDD92 pKa = 3.76AQIVSAAYY100 pKa = 9.74AATDD104 pKa = 4.1DD105 pKa = 4.13IYY107 pKa = 11.43TVVDD111 pKa = 3.02NDD113 pKa = 3.61KK114 pKa = 10.57RR115 pKa = 11.84VSSEE119 pKa = 4.03KK120 pKa = 10.52VDD122 pKa = 3.74VLQVTYY128 pKa = 10.54AVSDD132 pKa = 3.51SGSYY136 pKa = 8.82TGKK139 pKa = 10.66KK140 pKa = 9.74KK141 pKa = 8.71ITRR144 pKa = 11.84AEE146 pKa = 4.04EE147 pKa = 3.94LLSMFLCSDD156 pKa = 3.72SNYY159 pKa = 9.46WAYY162 pKa = 11.13LGG164 pKa = 4.3

Molecular weight: 18.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9U6X0|R9U6X0_9CAUD Uncharacterized protein OS=Vibrio phage jenny 12G5 OX=573176 GN=VPJG_00041 PE=4 SV=1
MM1 pKa = 7.64SAQHH5 pKa = 4.53QQARR9 pKa = 11.84QRR11 pKa = 11.84NNKK14 pKa = 8.56HH15 pKa = 5.26KK16 pKa = 10.22HH17 pKa = 4.95HH18 pKa = 6.46SQWTYY23 pKa = 10.37PEE25 pKa = 4.01HH26 pKa = 5.73TRR28 pKa = 11.84HH29 pKa = 5.27VRR31 pKa = 11.84QDD33 pKa = 2.6EE34 pKa = 4.32WQNLMLEE41 pKa = 4.42VIRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.95IIAMM50 pKa = 4.27

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

12239

44

899

165.4

18.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.378 ± 0.441

1.487 ± 0.155

6.21 ± 0.254

7.296 ± 0.33

3.603 ± 0.2

6.536 ± 0.286

1.773 ± 0.163

5.785 ± 0.229

7.345 ± 0.508

7.313 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.255

5.703 ± 0.262

3.121 ± 0.183

4.118 ± 0.333

4.379 ± 0.214

7.166 ± 0.284

6.177 ± 0.415

6.708 ± 0.313

1.381 ± 0.141

3.791 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski