Escherichia phage ZCEC5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W546|A0A481W546_9CAUD Uncharacterized protein OS=Escherichia phage ZCEC5 OX=2530021 PE=4 SV=1
MM1 pKa = 6.44VQRR4 pKa = 11.84YY5 pKa = 7.19TYY7 pKa = 10.55DD8 pKa = 3.1QIFGFTPDD16 pKa = 3.29VEE18 pKa = 4.7GVLVLYY24 pKa = 10.47EE25 pKa = 4.92DD26 pKa = 4.13YY27 pKa = 11.5AKK29 pKa = 11.08LEE31 pKa = 4.35AEE33 pKa = 3.97LQKK36 pKa = 11.37YY37 pKa = 9.63KK38 pKa = 10.98DD39 pKa = 3.48QFPDD43 pKa = 3.4YY44 pKa = 10.69VEE46 pKa = 4.48CANCGSVTHH55 pKa = 5.71VEE57 pKa = 4.27GVEE60 pKa = 3.87

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W5F1|A0A481W5F1_9CAUD Uncharacterized protein OS=Escherichia phage ZCEC5 OX=2530021 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.35ILKK9 pKa = 10.63RR10 pKa = 11.84SGLSPLSPRR19 pKa = 11.84AKK21 pKa = 7.78TQIYY25 pKa = 9.57KK26 pKa = 10.42RR27 pKa = 11.84RR28 pKa = 11.84GNALYY33 pKa = 10.07PEE35 pKa = 4.05IQARR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 9.87AIRR44 pKa = 11.84ACGFQNGKK52 pKa = 9.72AVNLGEE58 pKa = 4.7FKK60 pKa = 9.58TQEE63 pKa = 3.57RR64 pKa = 11.84AAIANRR70 pKa = 11.84LFNYY74 pKa = 7.62WKK76 pKa = 10.65SLGYY80 pKa = 10.66DD81 pKa = 4.56DD82 pKa = 6.25IPTKK86 pKa = 8.88PQRR89 pKa = 11.84RR90 pKa = 11.84QYY92 pKa = 9.93IWRR95 pKa = 11.84HH96 pKa = 4.21KK97 pKa = 10.55

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13079

39

851

181.7

20.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.764 ± 0.544

1.155 ± 0.191

6.185 ± 0.249

6.988 ± 0.383

3.861 ± 0.151

7.615 ± 0.26

1.621 ± 0.177

5.291 ± 0.175

5.727 ± 0.379

7.424 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.523 ± 0.139

4.221 ± 0.236

3.854 ± 0.227

3.831 ± 0.28

5.536 ± 0.27

5.727 ± 0.271

6.491 ± 0.398

7.172 ± 0.274

1.529 ± 0.16

3.487 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski