Eubacterium hallii CAG:12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6GWN1|R6GWN1_9FIRM Carbohydrate kinase FGGY family protein OS=Eubacterium hallii CAG:12 OX=1263078 GN=BN476_01919 PE=4 SV=1
MM1 pKa = 7.56FKK3 pKa = 10.98NKK5 pKa = 9.74LKK7 pKa = 10.47RR8 pKa = 11.84LCAVTLAGMMVMSVSACSTSKK29 pKa = 10.56SAEE32 pKa = 3.99KK33 pKa = 10.78NSVSKK38 pKa = 11.23AEE40 pKa = 4.1QASEE44 pKa = 4.05ATTEE48 pKa = 3.93AAKK51 pKa = 10.8DD52 pKa = 3.64DD53 pKa = 3.81TTADD57 pKa = 3.72YY58 pKa = 10.81VASGVEE64 pKa = 4.01LPDD67 pKa = 3.34NFEE70 pKa = 4.0NMIYY74 pKa = 10.14PIEE77 pKa = 4.35ALMLQNYY84 pKa = 9.47SKK86 pKa = 10.97GYY88 pKa = 8.89PYY90 pKa = 10.21YY91 pKa = 11.17ANGASEE97 pKa = 4.5EE98 pKa = 4.32KK99 pKa = 10.41SDD101 pKa = 4.44SFWFSMAALTSLMEE115 pKa = 4.23NEE117 pKa = 4.07SAYY120 pKa = 10.81GDD122 pKa = 4.38GIEE125 pKa = 4.11MDD127 pKa = 3.16SYY129 pKa = 11.97YY130 pKa = 10.8YY131 pKa = 10.93LKK133 pKa = 10.89EE134 pKa = 4.03NTSNMYY140 pKa = 10.93ASALYY145 pKa = 9.87NAYY148 pKa = 10.44GMGNMEE154 pKa = 4.84FPEE157 pKa = 5.02IPDD160 pKa = 3.35GDD162 pKa = 3.74KK163 pKa = 11.08YY164 pKa = 10.96ATYY167 pKa = 10.99DD168 pKa = 4.61DD169 pKa = 4.19GKK171 pKa = 11.19QMYY174 pKa = 9.67GFLKK178 pKa = 10.84GEE180 pKa = 4.16VSSLVPYY187 pKa = 9.07ITNCVKK193 pKa = 10.81DD194 pKa = 3.87GSDD197 pKa = 4.16YY198 pKa = 10.88IITSQLRR205 pKa = 11.84DD206 pKa = 3.39KK207 pKa = 10.92DD208 pKa = 3.51TDD210 pKa = 3.83EE211 pKa = 4.41VKK213 pKa = 9.72GTYY216 pKa = 9.4EE217 pKa = 3.87VTITASSFDD226 pKa = 3.64GDD228 pKa = 4.52DD229 pKa = 3.16NCFAYY234 pKa = 10.46SATAIRR240 pKa = 11.84QIGEE244 pKa = 4.15TDD246 pKa = 3.61SEE248 pKa = 4.36NQSTSSEE255 pKa = 4.21SEE257 pKa = 3.96EE258 pKa = 4.2ASTDD262 pKa = 3.54STSEE266 pKa = 4.03NGDD269 pKa = 3.43STDD272 pKa = 4.1ANSSTDD278 pKa = 3.29STDD281 pKa = 3.22ATDD284 pKa = 3.78STDD287 pKa = 3.28STEE290 pKa = 6.19GISQDD295 pKa = 4.6DD296 pKa = 4.32ALSQAKK302 pKa = 9.96DD303 pKa = 3.72YY304 pKa = 11.04YY305 pKa = 11.44GEE307 pKa = 4.3DD308 pKa = 3.16AEE310 pKa = 4.38YY311 pKa = 10.51SYY313 pKa = 11.26KK314 pKa = 10.72KK315 pKa = 10.29QVTVGDD321 pKa = 3.51KK322 pKa = 10.09EE323 pKa = 4.44YY324 pKa = 11.32YY325 pKa = 10.11DD326 pKa = 4.55FSVSGDD332 pKa = 3.55NVSSTDD338 pKa = 3.25VLVSVDD344 pKa = 3.61GQDD347 pKa = 4.62VIGGTQNDD355 pKa = 3.84DD356 pKa = 3.78GSWSFDD362 pKa = 3.2QQ363 pKa = 5.08

Molecular weight: 39.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6G9U3|R6G9U3_9FIRM Uncharacterized protein OS=Eubacterium hallii CAG:12 OX=1263078 GN=BN476_01577 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84NKK41 pKa = 10.44LSAA44 pKa = 3.84

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2309

0

2309

735250

29

2058

318.4

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.305 ± 0.056

1.514 ± 0.02

5.461 ± 0.041

7.857 ± 0.056

4.103 ± 0.034

7.004 ± 0.054

1.787 ± 0.024

7.728 ± 0.047

7.95 ± 0.051

8.64 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.084 ± 0.024

4.465 ± 0.035

3.155 ± 0.027

3.15 ± 0.033

4.002 ± 0.037

5.794 ± 0.042

5.363 ± 0.037

6.789 ± 0.044

0.781 ± 0.015

4.067 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski