Enterococcus phage phiSHEF4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; Enterococcus virus phiSHEF4

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XVT6|A0A249XVT6_9CAUD Uncharacterized protein OS=Enterococcus phage phiSHEF4 OX=2030923 GN=phiSHEF4_53 PE=4 SV=1
MM1 pKa = 6.19TTKK4 pKa = 10.18TNYY7 pKa = 10.08HH8 pKa = 5.89ICLTNNEE15 pKa = 4.26YY16 pKa = 10.96FNLYY20 pKa = 8.63TEE22 pKa = 4.36EE23 pKa = 5.61PILVMYY29 pKa = 8.19EE30 pKa = 3.77QAVEE34 pKa = 4.0NDD36 pKa = 3.25EE37 pKa = 4.95KK38 pKa = 11.19LLKK41 pKa = 10.46LEE43 pKa = 4.17KK44 pKa = 10.38PEE46 pKa = 4.64AIEE49 pKa = 4.75VDD51 pKa = 4.21GEE53 pKa = 4.27MQDD56 pKa = 3.46TFITIPLDD64 pKa = 3.76SILYY68 pKa = 9.2VLEE71 pKa = 4.37DD72 pKa = 3.47AKK74 pKa = 11.4

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XUK3|A0A249XUK3_9CAUD Uncharacterized protein OS=Enterococcus phage phiSHEF4 OX=2030923 GN=phiSHEF4_34 PE=4 SV=1
MM1 pKa = 7.64SNLKK5 pKa = 8.5TFKK8 pKa = 9.53VTYY11 pKa = 10.58GNIEE15 pKa = 4.13NKK17 pKa = 8.16VQRR20 pKa = 11.84AYY22 pKa = 11.25LKK24 pKa = 10.09AYY26 pKa = 9.59HH27 pKa = 6.2YY28 pKa = 11.33NEE30 pKa = 3.83ALRR33 pKa = 11.84RR34 pKa = 11.84AKK36 pKa = 10.26RR37 pKa = 11.84INGLSCIIKK46 pKa = 10.53LEE48 pKa = 4.23VVKK51 pKa = 11.06

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12342

46

1456

195.9

22.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.591 ± 0.405

0.624 ± 0.126

6.093 ± 0.216

8.078 ± 0.519

4.076 ± 0.221

6.725 ± 0.639

1.564 ± 0.169

6.895 ± 0.243

8.872 ± 0.354

8.281 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.192

6.45 ± 0.297

2.893 ± 0.201

3.614 ± 0.204

3.533 ± 0.189

5.38 ± 0.336

6.352 ± 0.316

6.684 ± 0.268

1.288 ± 0.138

4.011 ± 0.331
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski