Rotavirus D

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0LBN7|A0A0K0LBN7_9REOV VP3 (Fragment) OS=Rotavirus D OX=335100 GN=VP3 PE=4 SV=1
SS1 pKa = 6.58SRR3 pKa = 11.84AGTSYY8 pKa = 10.59TRR10 pKa = 11.84IDD12 pKa = 3.78YY13 pKa = 11.11DD14 pKa = 3.98EE15 pKa = 6.18DD16 pKa = 3.62MLLDD20 pKa = 5.8DD21 pKa = 4.82ITPSDD26 pKa = 3.88SASSQDD32 pKa = 3.53TNQKK36 pKa = 7.09TFRR39 pKa = 11.84EE40 pKa = 4.23KK41 pKa = 10.69SFKK44 pKa = 9.69SSSMISQCDD53 pKa = 3.19EE54 pKa = 4.36DD55 pKa = 6.6DD56 pKa = 4.04IANQEE61 pKa = 4.11MNKK64 pKa = 10.23LEE66 pKa = 4.52SIVNSTQIDD75 pKa = 3.82EE76 pKa = 4.53RR77 pKa = 11.84QNIADD82 pKa = 3.25WDD84 pKa = 4.06EE85 pKa = 3.85YY86 pKa = 11.34LEE88 pKa = 4.45RR89 pKa = 11.84NSGIKK94 pKa = 9.44IIKK97 pKa = 9.5GKK99 pKa = 10.64DD100 pKa = 3.38YY101 pKa = 10.55MDD103 pKa = 3.89KK104 pKa = 10.5TDD106 pKa = 3.76SDD108 pKa = 4.13GMLKK112 pKa = 10.59DD113 pKa = 3.12KK114 pKa = 10.04TLNYY118 pKa = 10.81SAMKK122 pKa = 9.48EE123 pKa = 4.27SNSIDD128 pKa = 3.42STVKK132 pKa = 10.56RR133 pKa = 11.84KK134 pKa = 9.84INSSKK139 pKa = 10.71INTQDD144 pKa = 2.94TSSDD148 pKa = 3.62EE149 pKa = 4.17EE150 pKa = 4.41CNRR153 pKa = 11.84NCKK156 pKa = 9.7CCKK159 pKa = 9.04KK160 pKa = 10.24LRR162 pKa = 11.84RR163 pKa = 11.84LRR165 pKa = 11.84KK166 pKa = 9.6RR167 pKa = 11.84MSILIAEE174 pKa = 4.76SYY176 pKa = 10.62

Molecular weight: 20.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0LBP2|A0A0K0LBP2_9REOV Non-structural protein 2 (Fragment) OS=Rotavirus D OX=335100 GN=NSP2 PE=3 SV=1
KKK2 pKa = 10.13INILLTTKKK11 pKa = 10.65DDD13 pKa = 3.6DD14 pKa = 4.39DD15 pKa = 4.92PIDDD19 pKa = 3.79IIYYY23 pKa = 9.91LTPIPKKK30 pKa = 8.37YY31 pKa = 10.38KK32 pKa = 10.31RR33 pKa = 11.84FASDDD38 pKa = 3.38SPSGINYYY46 pKa = 10.08DD47 pKa = 3.57EEE49 pKa = 4.25FDDD52 pKa = 5.85KK53 pKa = 10.69SSKKK57 pKa = 10.57AEEE60 pKa = 4.04LNFGVKKK67 pKa = 9.91KK68 pKa = 10.37SIQQAMMMLTRR79 pKa = 11.84VFSVRR84 pKa = 11.84HHH86 pKa = 5.67EEE88 pKa = 3.92LYYY91 pKa = 10.66YY92 pKa = 10.97MMDDD96 pKa = 3.38KK97 pKa = 11.16DD98 pKa = 4.27DD99 pKa = 4.97IYYY102 pKa = 10.71EE103 pKa = 4.33IDDD106 pKa = 3.52QFKKK110 pKa = 10.7VMVMLKKK117 pKa = 8.82TKKK120 pKa = 10.36FEEE123 pKa = 4.18TITAEEE129 pKa = 4.3GKKK132 pKa = 9.83LYYY135 pKa = 10.06DDD137 pKa = 3.38KK138 pKa = 10.75YYY140 pKa = 11.39LWEEE144 pKa = 3.97DDD146 pKa = 3.27YY147 pKa = 11.16KKK149 pKa = 10.38YY150 pKa = 11.46KK151 pKa = 10.56MPVKKK156 pKa = 10.33VEEE159 pKa = 4.06YY160 pKa = 10.16KK161 pKa = 9.1TLNSVTEEE169 pKa = 4.37DD170 pKa = 3.39DDD172 pKa = 3.99EEE174 pKa = 4.52KKK176 pKa = 7.52AAYYY180 pKa = 9.86KKK182 pKa = 9.12YY183 pKa = 9.75HH184 pKa = 5.28YY185 pKa = 10.89RR186 pKa = 11.84FAVISHHH193 pKa = 6.35FGHHH197 pKa = 6.14YY198 pKa = 10.22LVPYYY203 pKa = 10.16QVLNHHH209 pKa = 6.71EEE211 pKa = 4.17TFATYYY217 pKa = 10.74AIKKK221 pKa = 10.38KK222 pKa = 7.78KKK224 pKa = 9.38DDD226 pKa = 3.28HH227 pKa = 6.32FVRR230 pKa = 11.84LSKKK234 pKa = 9.79LLNTNWQDDD243 pKa = 2.97MNAVEEE249 pKa = 4.37YY250 pKa = 9.86KKK252 pKa = 11.32MSEEE256 pKa = 4.26RR257 pKa = 11.84

Molecular weight: 30.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5448

120

1051

495.3

57.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.956 ± 0.423

1.248 ± 0.414

6.406 ± 0.214

5.36 ± 0.357

4.167 ± 0.342

3.231 ± 0.289

1.523 ± 0.22

8.26 ± 0.358

6.278 ± 0.685

9.416 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.9 ± 0.366

7.36 ± 0.301

2.863 ± 0.376

3.781 ± 0.248

5.084 ± 0.406

7.93 ± 0.614

6.828 ± 0.365

6.076 ± 0.334

0.698 ± 0.135

5.635 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski