Mycobacterium phage Mowgli

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Liefievirus; unclassified Liefievirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KTB2|A0A345KTB2_9CAUD Uncharacterized protein OS=Mycobacterium phage Mowgli OX=2250406 GN=36 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 9.05GTLGALCGLAGDD14 pKa = 4.44ALIAVGFALRR24 pKa = 11.84EE25 pKa = 3.98HH26 pKa = 7.08DD27 pKa = 4.93RR28 pKa = 11.84GFSDD32 pKa = 5.1YY33 pKa = 11.15EE34 pKa = 4.91AGDD37 pKa = 3.85YY38 pKa = 7.45LTPAEE43 pKa = 4.71SPAPADD49 pKa = 4.23DD50 pKa = 4.52LSIPLGDD57 pKa = 3.46VRR59 pKa = 11.84AALDD63 pKa = 4.04DD64 pKa = 4.62LSDD67 pKa = 3.91SKK69 pKa = 11.36LLNIAATIIAGWKK82 pKa = 9.54PILLKK87 pKa = 8.89TTGDD91 pKa = 3.55LTDD94 pKa = 3.23VDD96 pKa = 4.41VLVDD100 pKa = 3.79ALRR103 pKa = 11.84DD104 pKa = 3.36RR105 pKa = 11.84ATQYY109 pKa = 11.76AVVEE113 pKa = 4.33LDD115 pKa = 4.38DD116 pKa = 4.48VPPADD121 pKa = 3.91GAPNNSPEE129 pKa = 4.07RR130 pKa = 11.84GEE132 pKa = 3.93

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KTB5|A0A345KTB5_9CAUD Uncharacterized protein OS=Mycobacterium phage Mowgli OX=2250406 GN=39 PE=4 SV=1
MM1 pKa = 6.63TTVHH5 pKa = 6.69DD6 pKa = 4.34VKK8 pKa = 10.79IEE10 pKa = 3.82PRR12 pKa = 11.84WFDD15 pKa = 4.97RR16 pKa = 11.84IQQSEE21 pKa = 4.17KK22 pKa = 9.94FAEE25 pKa = 4.01VRR27 pKa = 11.84YY28 pKa = 10.34DD29 pKa = 3.75DD30 pKa = 5.0RR31 pKa = 11.84DD32 pKa = 3.63YY33 pKa = 11.55QAGDD37 pKa = 4.02RR38 pKa = 11.84IRR40 pKa = 11.84FWRR43 pKa = 11.84TDD45 pKa = 3.14RR46 pKa = 11.84PEE48 pKa = 4.1YY49 pKa = 9.89GHH51 pKa = 6.64SIEE54 pKa = 5.25RR55 pKa = 11.84RR56 pKa = 11.84ITHH59 pKa = 5.82VLRR62 pKa = 11.84GVDD65 pKa = 3.87GLRR68 pKa = 11.84DD69 pKa = 3.95GYY71 pKa = 11.4VVLSLSDD78 pKa = 3.52PRR80 pKa = 11.84LDD82 pKa = 3.58QLRR85 pKa = 11.84GRR87 pKa = 11.84LDD89 pKa = 3.21RR90 pKa = 11.84ALDD93 pKa = 3.69EE94 pKa = 4.16NSRR97 pKa = 11.84LARR100 pKa = 11.84SNRR103 pKa = 11.84GLRR106 pKa = 11.84GANTRR111 pKa = 11.84LRR113 pKa = 11.84NARR116 pKa = 3.55

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13763

49

1337

218.5

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.231 ± 0.586

0.966 ± 0.163

6.953 ± 0.336

5.595 ± 0.395

2.928 ± 0.175

9.046 ± 0.57

2.281 ± 0.246

4.389 ± 0.2

3.517 ± 0.302

7.513 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.11

2.957 ± 0.161

6.154 ± 0.428

3.488 ± 0.189

7.062 ± 0.485

5.181 ± 0.308

6.016 ± 0.264

6.714 ± 0.226

1.947 ± 0.161

2.027 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski