Campylobacter sp. MIT 12-5580
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1777 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U7BFL7|A0A4U7BFL7_9PROT TonB-dependent receptor OS=Campylobacter sp. MIT 12-5580 OX=2040651 GN=CQA38_07235 PE=4 SV=1
MM1 pKa = 8.04 RR2 pKa = 11.84 EE3 pKa = 3.76 FEE5 pKa = 4.68 DD6 pKa = 6.45 DD7 pKa = 3.69 EE8 pKa = 6.27 FEE10 pKa = 5.82 DD11 pKa = 6.44 LDD13 pKa = 4.21 DD14 pKa = 5.14 QDD16 pKa = 3.48 ILSYY20 pKa = 10.95 EE21 pKa = 4.47 DD22 pKa = 3.42 EE23 pKa = 4.88 DD24 pKa = 4.99 DD25 pKa = 3.98 YY26 pKa = 11.89 LRR28 pKa = 11.84 SEE30 pKa = 4.01 HH31 pKa = 6.22 SRR33 pKa = 11.84 NYY35 pKa = 10.93 NYY37 pKa = 10.92 DD38 pKa = 3.31 EE39 pKa = 5.53 DD40 pKa = 5.61 DD41 pKa = 4.02 YY42 pKa = 12.11 NFDD45 pKa = 5.17 DD46 pKa = 4.49 EE47 pKa = 6.56 DD48 pKa = 5.43 YY49 pKa = 11.11 DD50 pKa = 4.71 DD51 pKa = 4.6 SYY53 pKa = 12.23 EE54 pKa = 4.06 MEE56 pKa = 4.11
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.085
IPC2_protein 3.3
IPC_protein 3.287
Toseland 3.083
ProMoST 3.465
Dawson 3.3
Bjellqvist 3.516
Wikipedia 3.261
Rodwell 3.121
Grimsley 2.994
Solomon 3.261
Lehninger 3.223
Nozaki 3.439
DTASelect 3.643
Thurlkill 3.147
EMBOSS 3.274
Sillero 3.414
Patrickios 0.223
IPC_peptide 3.261
IPC2_peptide 3.389
IPC2.peptide.svr19 3.668
Protein with the highest isoelectric point:
>tr|A0A4U7BD90|A0A4U7BD90_9PROT Uncharacterized protein OS=Campylobacter sp. MIT 12-5580 OX=2040651 GN=CQA38_05020 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 5.76 KK9 pKa = 7.92 TPKK12 pKa = 9.96 KK13 pKa = 7.47 RR14 pKa = 11.84 THH16 pKa = 5.94 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.39 NGRR28 pKa = 11.84 KK29 pKa = 9.3 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1777
0
1777
557218
37
1944
313.6
35.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.602 ± 0.06
1.197 ± 0.022
5.069 ± 0.037
7.115 ± 0.068
6.163 ± 0.061
5.533 ± 0.066
1.726 ± 0.022
7.664 ± 0.055
9.234 ± 0.075
11.365 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.068 ± 0.024
5.531 ± 0.056
2.615 ± 0.032
3.559 ± 0.039
3.162 ± 0.035
6.866 ± 0.05
4.102 ± 0.048
5.263 ± 0.051
0.624 ± 0.015
3.541 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here