Methanomassiliicoccus intestinalis (strain Issoire-Mx1)

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; Methanomassiliicoccaceae; Methanomassiliicoccus; Candidatus Methanomassiliicoccus intestinalis

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9T7M1|R9T7M1_METII Translation initiation factor 5A OS=Methanomassiliicoccus intestinalis (strain Issoire-Mx1) OX=1295009 GN=eif5a PE=3 SV=1
MM1 pKa = 7.57NDD3 pKa = 2.86KK4 pKa = 10.76SKK6 pKa = 11.29NKK8 pKa = 10.07SIIMALAVFIAIAAFASPLLMSDD31 pKa = 5.05DD32 pKa = 4.37NGDD35 pKa = 3.68SNTVVLSDD43 pKa = 3.74EE44 pKa = 4.44APDD47 pKa = 3.82YY48 pKa = 11.5VSVSSQDD55 pKa = 4.63DD56 pKa = 3.27IDD58 pKa = 3.95NAAEE62 pKa = 4.01NSKK65 pKa = 10.79LLLNDD70 pKa = 4.44DD71 pKa = 4.08FTGSLTIPSGTSLYY85 pKa = 10.99GIDD88 pKa = 3.88IPKK91 pKa = 10.6DD92 pKa = 3.3LTDD95 pKa = 4.86LVGAPDD101 pKa = 3.83GAKK104 pKa = 9.9TITGDD109 pKa = 3.36VTLEE113 pKa = 3.98AGATLNFIKK122 pKa = 10.63INGKK126 pKa = 9.45VSICDD131 pKa = 3.41NNVTVNDD138 pKa = 3.7STATEE143 pKa = 3.75IRR145 pKa = 11.84YY146 pKa = 7.84CTINSEE152 pKa = 4.01TAIAISYY159 pKa = 10.88LDD161 pKa = 3.56IQAGDD166 pKa = 3.31ISIVSNEE173 pKa = 3.82INNSNTTNAILSFEE187 pKa = 4.29GARR190 pKa = 11.84DD191 pKa = 3.7GKK193 pKa = 10.69RR194 pKa = 11.84GATASEE200 pKa = 4.95SIFSLSISNNKK211 pKa = 9.63LGADD215 pKa = 3.54EE216 pKa = 5.1GRR218 pKa = 11.84AFSGNDD224 pKa = 3.22KK225 pKa = 11.02VGLSYY230 pKa = 11.22AGGSQISISNNIIADD245 pKa = 3.79APSWGASIMLRR256 pKa = 11.84SLNEE260 pKa = 3.4NSTFDD265 pKa = 3.45VSKK268 pKa = 11.53NNIANGDD275 pKa = 3.75KK276 pKa = 11.01PEE278 pKa = 4.16VGLSGGNAFDD288 pKa = 4.43GDD290 pKa = 4.29ANSFLDD296 pKa = 5.36GFTTDD301 pKa = 3.81AKK303 pKa = 11.14SLIIPIGSIAVLSEE317 pKa = 3.63GSEE320 pKa = 4.33FEE322 pKa = 4.19FEE324 pKa = 4.46RR325 pKa = 11.84ITVNGTITYY334 pKa = 9.76DD335 pKa = 3.21GSSISNVDD343 pKa = 3.73GISAIKK349 pKa = 10.35AGSLIINGEE358 pKa = 4.18LTPIGADD365 pKa = 3.36TPIVVSGDD373 pKa = 2.88ITLEE377 pKa = 3.99GSLMGKK383 pKa = 10.05LIILPEE389 pKa = 4.58EE390 pKa = 3.93IDD392 pKa = 3.27EE393 pKa = 4.92DD394 pKa = 4.77GNVLVKK400 pKa = 11.09SNIKK404 pKa = 9.97LRR406 pKa = 11.84NFEE409 pKa = 4.29TTDD412 pKa = 3.44GAVIEE417 pKa = 4.52YY418 pKa = 10.24KK419 pKa = 10.17LTVDD423 pKa = 4.92DD424 pKa = 4.98EE425 pKa = 5.28GSVSDD430 pKa = 4.56IGTISNVSEE439 pKa = 4.01IKK441 pKa = 10.01TNEE444 pKa = 3.75AGEE447 pKa = 4.36DD448 pKa = 3.67VVISNLYY455 pKa = 8.73EE456 pKa = 4.08DD457 pKa = 4.09RR458 pKa = 11.84GVIIDD463 pKa = 3.66VHH465 pKa = 7.79DD466 pKa = 3.42IEE468 pKa = 5.21YY469 pKa = 10.8NSEE472 pKa = 4.01YY473 pKa = 10.33QDD475 pKa = 3.65APVDD479 pKa = 3.79VTQFKK484 pKa = 10.26AANNIKK490 pKa = 9.43VLKK493 pKa = 9.86EE494 pKa = 3.36ITYY497 pKa = 10.22TNVCGAKK504 pKa = 9.9FVYY507 pKa = 10.2NVEE510 pKa = 5.04DD511 pKa = 4.16YY512 pKa = 9.19FTDD515 pKa = 4.41SILDD519 pKa = 3.59VNGISAKK526 pKa = 10.52VEE528 pKa = 4.53TIDD531 pKa = 4.11KK532 pKa = 10.3TSAFLNLKK540 pKa = 7.54VTNAGKK546 pKa = 10.79YY547 pKa = 7.79EE548 pKa = 3.85LTYY551 pKa = 10.37IVSTNAISDD560 pKa = 3.68DD561 pKa = 3.52GKK563 pKa = 10.81NIVDD567 pKa = 3.59SDD569 pKa = 4.0FKK571 pKa = 11.79VEE573 pKa = 4.05FTVSQKK579 pKa = 10.22VIKK582 pKa = 10.07YY583 pKa = 9.2ALPLVVKK590 pKa = 10.38IFDD593 pKa = 3.58GTTDD597 pKa = 3.03VFGNIVCLDD606 pKa = 3.41IFDD609 pKa = 4.32EE610 pKa = 4.95DD611 pKa = 3.77EE612 pKa = 4.31VSFLLEE618 pKa = 4.31FEE620 pKa = 4.53SADD623 pKa = 3.57AGIHH627 pKa = 5.35KK628 pKa = 10.58LIFKK632 pKa = 10.69GLDD635 pKa = 3.18GEE637 pKa = 4.78DD638 pKa = 3.13AGNYY642 pKa = 9.11ILADD646 pKa = 3.48YY647 pKa = 10.75YY648 pKa = 11.1FITNYY653 pKa = 8.75THH655 pKa = 7.34DD656 pKa = 3.91CCEE659 pKa = 3.85NSEE662 pKa = 4.16SCEE665 pKa = 4.19CGDD668 pKa = 5.44LPFSMDD674 pKa = 4.52DD675 pKa = 3.75CTCEE679 pKa = 5.32NDD681 pKa = 3.1FCMCKK686 pKa = 10.45FIFYY690 pKa = 10.98GLILPKK696 pKa = 10.19TITSDD701 pKa = 3.28MITISNVVPSFDD713 pKa = 4.71HH714 pKa = 7.0PDD716 pKa = 3.19KK717 pKa = 11.01VYY719 pKa = 8.69PTITVEE725 pKa = 4.33LLDD728 pKa = 4.07GTTVEE733 pKa = 5.24LDD735 pKa = 3.23TSKK738 pKa = 10.58MNEE741 pKa = 3.93RR742 pKa = 11.84DD743 pKa = 3.11YY744 pKa = 11.11DD745 pKa = 3.6IPFDD749 pKa = 3.78GFFVNKK755 pKa = 9.53NDD757 pKa = 3.47CEE759 pKa = 4.35QYY761 pKa = 11.07FEE763 pKa = 4.66FEE765 pKa = 4.79DD766 pKa = 5.09GISINDD772 pKa = 3.48AGDD775 pKa = 3.69YY776 pKa = 10.34YY777 pKa = 11.61AVICNHH783 pKa = 6.83PLAPSYY789 pKa = 11.28NMNYY793 pKa = 9.85SSSFSGSNYY802 pKa = 8.59IAGPILEE809 pKa = 4.74NIFYY813 pKa = 10.3EE814 pKa = 4.37YY815 pKa = 10.11IEE817 pKa = 4.01NVIYY821 pKa = 9.8EE822 pKa = 4.69LPDD825 pKa = 3.48ATDD828 pKa = 3.11NGMDD832 pKa = 3.73NSIPMYY838 pKa = 9.96LTDD841 pKa = 5.99LLTQFISHH849 pKa = 6.5FTNNGEE855 pKa = 4.31SVNIQDD861 pKa = 5.37IINLLFTLPINGEE874 pKa = 3.98DD875 pKa = 3.73SGNKK879 pKa = 9.99AIVDD883 pKa = 3.22IDD885 pKa = 3.79DD886 pKa = 3.54RR887 pKa = 11.84TLFMMYY893 pKa = 9.99YY894 pKa = 10.01LASAEE899 pKa = 4.12EE900 pKa = 3.97NANLKK905 pKa = 10.94GEE907 pKa = 4.04LAYY910 pKa = 10.68DD911 pKa = 3.98GGNNIIRR918 pKa = 11.84FSEE921 pKa = 4.09DD922 pKa = 2.97LKK924 pKa = 11.58NEE926 pKa = 4.01AGLRR930 pKa = 11.84AWYY933 pKa = 10.33LSFNNQLSEE942 pKa = 4.23FGEE945 pKa = 4.4GDD947 pKa = 3.26YY948 pKa = 10.98HH949 pKa = 8.48LRR951 pKa = 11.84ISADD955 pKa = 3.19DD956 pKa = 3.7VTIAEE961 pKa = 4.36YY962 pKa = 10.19TFTFSSDD969 pKa = 3.71GIDD972 pKa = 4.45DD973 pKa = 4.2IVNVIYY979 pKa = 10.62DD980 pKa = 3.56EE981 pKa = 5.79GYY983 pKa = 11.36ALDD986 pKa = 5.26FANAWASKK994 pKa = 10.41KK995 pKa = 10.56GFEE998 pKa = 4.35SKK1000 pKa = 10.58SGIVINYY1007 pKa = 9.17HH1008 pKa = 6.23YY1009 pKa = 10.45IYY1011 pKa = 9.98DD1012 pKa = 4.2DD1013 pKa = 3.61KK1014 pKa = 11.41GSEE1017 pKa = 3.61IRR1019 pKa = 11.84EE1020 pKa = 3.82EE1021 pKa = 4.33HH1022 pKa = 6.42IEE1024 pKa = 3.99QIINIAMLKK1033 pKa = 10.52NGLQYY1038 pKa = 10.57TYY1040 pKa = 11.2SNVGAQLQQYY1050 pKa = 9.17EE1051 pKa = 4.42FLRR1054 pKa = 11.84WSTVGSPSLYY1064 pKa = 10.8DD1065 pKa = 3.69DD1066 pKa = 4.07ACLVDD1071 pKa = 4.73NEE1073 pKa = 4.73LFHH1076 pKa = 7.27FNALEE1081 pKa = 4.37VIDD1084 pKa = 4.1RR1085 pKa = 11.84TDD1087 pKa = 3.46LEE1089 pKa = 4.68SYY1091 pKa = 10.91AVDD1094 pKa = 3.6GVLNLYY1100 pKa = 10.12AVYY1103 pKa = 10.92DD1104 pKa = 3.97MKK1106 pKa = 10.95TPKK1109 pKa = 9.7PDD1111 pKa = 3.17NKK1113 pKa = 10.95SIDD1116 pKa = 3.79NVAAFYY1122 pKa = 10.78DD1123 pKa = 3.55IDD1125 pKa = 3.82AKK1127 pKa = 10.69KK1128 pKa = 10.7VQDD1131 pKa = 3.75LMSLYY1136 pKa = 10.29GINVDD1141 pKa = 4.18DD1142 pKa = 4.74VISRR1146 pKa = 11.84TMAFIYY1152 pKa = 8.99EE1153 pKa = 3.88QSGYY1157 pKa = 11.43SNTTMKK1163 pKa = 10.95GILYY1167 pKa = 9.99KK1168 pKa = 10.69LVNGEE1173 pKa = 4.04LLEE1176 pKa = 4.65VYY1178 pKa = 10.53HH1179 pKa = 7.02EE1180 pKa = 5.39DD1181 pKa = 4.7NLNSDD1186 pKa = 3.81DD1187 pKa = 5.94GLRR1190 pKa = 11.84AWYY1193 pKa = 9.43FSFDD1197 pKa = 3.74DD1198 pKa = 3.96QVNSAVDD1205 pKa = 3.57TYY1207 pKa = 11.71GLAGYY1212 pKa = 10.18YY1213 pKa = 9.58DD1214 pKa = 3.88ARR1216 pKa = 11.84IVAVDD1221 pKa = 3.36PEE1223 pKa = 4.52GNEE1226 pKa = 4.19TEE1228 pKa = 4.07LLSAKK1233 pKa = 9.73FHH1235 pKa = 6.41IAEE1238 pKa = 4.29EE1239 pKa = 4.6LYY1241 pKa = 10.71GANITVDD1248 pKa = 3.79YY1249 pKa = 11.43VNVDD1253 pKa = 4.44DD1254 pKa = 5.86INDD1257 pKa = 3.57AVKK1260 pKa = 10.81VYY1262 pKa = 10.4IEE1264 pKa = 4.51PKK1266 pKa = 10.2NGCLVPAGDD1275 pKa = 5.34LIVAYY1280 pKa = 10.47SFMSSGPFGDD1290 pKa = 4.42EE1291 pKa = 3.87PASALADD1298 pKa = 3.41AVPIPVSSSTQIIDD1312 pKa = 3.35VDD1314 pKa = 3.46ISGMDD1319 pKa = 3.39SANAIFKK1326 pKa = 9.85WIDD1329 pKa = 3.38SSGHH1333 pKa = 4.33TVYY1336 pKa = 11.03SLSNTVTSQQ1345 pKa = 2.73

Molecular weight: 146.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TAI4|R9TAI4_METII 7-cyano-7-deazaguanine synthase OS=Methanomassiliicoccus intestinalis (strain Issoire-Mx1) OX=1295009 GN=queC PE=3 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84KK4 pKa = 8.58PNRR7 pKa = 11.84MYY9 pKa = 10.76RR10 pKa = 11.84QIRR13 pKa = 11.84GQAYY17 pKa = 6.57TRR19 pKa = 11.84RR20 pKa = 11.84EE21 pKa = 4.01YY22 pKa = 10.51MGGVPANRR30 pKa = 11.84ISTYY34 pKa = 11.06NLGNVTGDD42 pKa = 3.29FPIVMNLRR50 pKa = 11.84IKK52 pKa = 9.85EE53 pKa = 4.11PCQIRR58 pKa = 11.84HH59 pKa = 5.44TALEE63 pKa = 4.03AARR66 pKa = 11.84IAANRR71 pKa = 11.84ALSKK75 pKa = 10.01GTTSASYY82 pKa = 10.18HH83 pKa = 5.18LRR85 pKa = 11.84IRR87 pKa = 11.84LYY89 pKa = 7.95PHH91 pKa = 6.13IVLRR95 pKa = 11.84EE96 pKa = 3.92HH97 pKa = 7.26KK98 pKa = 9.02MATGAGADD106 pKa = 3.4RR107 pKa = 11.84VSGGMRR113 pKa = 11.84SAFGKK118 pKa = 10.64CVGTACRR125 pKa = 11.84VEE127 pKa = 4.35SGTAIITLRR136 pKa = 11.84VTPQTFQLGKK146 pKa = 9.24NALWKK151 pKa = 10.77ASMKK155 pKa = 10.57LPSPCQIEE163 pKa = 4.17IEE165 pKa = 4.21KK166 pKa = 10.68GAEE169 pKa = 3.78LVAA172 pKa = 5.36

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1826

0

1826

562683

38

5834

308.2

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.396 ± 0.066

1.537 ± 0.041

6.217 ± 0.054

6.847 ± 0.076

3.727 ± 0.038

7.232 ± 0.091

1.626 ± 0.026

8.09 ± 0.054

6.455 ± 0.057

8.584 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.912 ± 0.047

4.593 ± 0.083

3.721 ± 0.033

2.367 ± 0.031

4.01 ± 0.069

7.474 ± 0.068

5.5 ± 0.096

7.065 ± 0.047

0.94 ± 0.02

3.704 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski