Faecalibacterium sp. CAG:82
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1856 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6R281|R6R281_9FIRM Tripartite ATP-independent periplasmic transporter solute receptor DctP family OS=Faecalibacterium sp. CAG:82 OX=1262898 GN=BN792_01386 PE=4 SV=1
MM1 pKa = 7.84 ALPSDD6 pKa = 5.18 PIMLLSVVNLKK17 pKa = 10.59 LRR19 pKa = 11.84 DD20 pKa = 4.32 FYY22 pKa = 11.11 PSLDD26 pKa = 3.97 ALCDD30 pKa = 4.28 DD31 pKa = 5.41 LDD33 pKa = 5.41 ADD35 pKa = 4.05 QTALCASLAAVGYY48 pKa = 10.05 EE49 pKa = 3.61 YY50 pKa = 11.08 DD51 pKa = 3.19 AARR54 pKa = 11.84 NQFVV58 pKa = 2.98
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.732
IPC_protein 3.643
Toseland 3.439
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.719
Rodwell 3.49
Grimsley 3.35
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.783
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|R6QEG3|R6QEG3_9FIRM Uncharacterized protein OS=Faecalibacterium sp. CAG:82 OX=1262898 GN=BN792_01657 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.47 KK9 pKa = 7.63 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 7.09 EE14 pKa = 3.35 VHH16 pKa = 5.99 GFLTRR21 pKa = 11.84 MSTKK25 pKa = 10.35 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.97 SLTVV44 pKa = 3.12
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.774
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1856
0
1856
587560
29
1679
316.6
34.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.593 ± 0.073
1.778 ± 0.025
5.39 ± 0.043
6.528 ± 0.051
3.699 ± 0.038
7.911 ± 0.045
1.927 ± 0.026
5.263 ± 0.054
4.95 ± 0.05
10.079 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.028
3.428 ± 0.039
4.206 ± 0.037
3.568 ± 0.04
5.066 ± 0.055
5.325 ± 0.05
5.708 ± 0.039
7.378 ± 0.047
1.019 ± 0.02
3.402 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here