Tomato leaf curl Mayotte virus
Average proteome isoelectric point is 7.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5DVB5|Q5DVB5_9GEMI Transcriptional activator protein OS=Tomato leaf curl Mayotte virus OX=302100 PE=3 SV=1
MM1 pKa = 6.95 YY2 pKa = 9.64 RR3 pKa = 11.84 YY4 pKa = 9.35 PEE6 pKa = 4.13 KK7 pKa = 10.56 KK8 pKa = 9.06 RR9 pKa = 11.84 EE10 pKa = 4.01 LVNRR14 pKa = 11.84 YY15 pKa = 8.52 PLTNMPSAPKK25 pKa = 10.15 KK26 pKa = 9.77 FQINAKK32 pKa = 10.1 NIFLTYY38 pKa = 8.76 PQCTLSKK45 pKa = 10.94 EE46 pKa = 4.23 EE47 pKa = 4.42 TLSQIQNLSLPSNPKK62 pKa = 9.72 FIKK65 pKa = 9.66 ICKK68 pKa = 8.82 EE69 pKa = 3.39 LHH71 pKa = 6.02 QNGEE75 pKa = 3.97 PHH77 pKa = 6.32 IHH79 pKa = 6.11 MFIQFEE85 pKa = 4.28 GKK87 pKa = 9.52 IRR89 pKa = 11.84 ITNCRR94 pKa = 11.84 LFDD97 pKa = 4.02 LVSPTRR103 pKa = 11.84 SVHH106 pKa = 5.0 FHH108 pKa = 6.81 PNIQGAKK115 pKa = 9.25 SSSDD119 pKa = 3.11 VKK121 pKa = 11.24 SYY123 pKa = 10.79 IDD125 pKa = 3.7 KK126 pKa = 11.33 DD127 pKa = 3.49 GDD129 pKa = 3.67 TLEE132 pKa = 4.14 WGEE135 pKa = 3.84 FQIDD139 pKa = 3.21 GRR141 pKa = 11.84 SARR144 pKa = 11.84 GGQQSANDD152 pKa = 3.81 AYY154 pKa = 10.7 AAALNTGSKK163 pKa = 10.3 SEE165 pKa = 3.9 ALRR168 pKa = 11.84 VIKK171 pKa = 10.2 EE172 pKa = 3.88 LAPKK176 pKa = 10.6 DD177 pKa = 3.7 FVLQFHH183 pKa = 6.68 NLNSNLDD190 pKa = 4.08 RR191 pKa = 11.84 IFQEE195 pKa = 4.33 PPAPYY200 pKa = 9.57 ISPFLSSSFNQVPEE214 pKa = 4.17 EE215 pKa = 4.05 LEE217 pKa = 3.68 EE218 pKa = 4.19 WACEE222 pKa = 4.05 NVMDD226 pKa = 5.57 AAARR230 pKa = 11.84 PWRR233 pKa = 11.84 PISIVIEE240 pKa = 4.23 GDD242 pKa = 3.2 SRR244 pKa = 11.84 TGKK247 pKa = 8.52 TMWARR252 pKa = 11.84 SLGPHH257 pKa = 6.71 NYY259 pKa = 10.18 LCGHH263 pKa = 7.38 LDD265 pKa = 4.18 LSPKK269 pKa = 10.15 VYY271 pKa = 11.07 SNDD274 pKa = 2.25 AWYY277 pKa = 10.92 NVIDD281 pKa = 5.2 DD282 pKa = 4.41 VDD284 pKa = 3.61 PHH286 pKa = 5.85 YY287 pKa = 11.0 LKK289 pKa = 10.7 HH290 pKa = 6.2 FKK292 pKa = 10.7 EE293 pKa = 4.46 FMGAQRR299 pKa = 11.84 DD300 pKa = 3.81 WQSNTKK306 pKa = 9.26 YY307 pKa = 10.63 GKK309 pKa = 9.49 PIQIKK314 pKa = 10.42 GGIPTIFLCNPGPTSSYY331 pKa = 11.31 KK332 pKa = 10.67 EE333 pKa = 3.89 FLDD336 pKa = 3.83 EE337 pKa = 4.65 EE338 pKa = 4.54 KK339 pKa = 10.79 NAPLRR344 pKa = 11.84 DD345 pKa = 3.51 WAVKK349 pKa = 9.66 NATFITLHH357 pKa = 6.17 EE358 pKa = 4.49 PLYY361 pKa = 10.14 STANQSPAQISQEE374 pKa = 4.2 EE375 pKa = 4.54 GSEE378 pKa = 3.96 AA379 pKa = 3.7
Molecular weight: 43.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.07
IPC2_protein 6.046
IPC_protein 6.135
Toseland 6.478
ProMoST 6.517
Dawson 6.453
Bjellqvist 6.427
Wikipedia 6.465
Rodwell 6.44
Grimsley 6.62
Solomon 6.453
Lehninger 6.453
Nozaki 6.722
DTASelect 6.898
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.839
Patrickios 4.202
IPC_peptide 6.465
IPC2_peptide 6.707
IPC2.peptide.svr19 6.719
Protein with the highest isoelectric point:
>tr|Q5DVB8|Q5DVB8_9GEMI Protein V2 OS=Tomato leaf curl Mayotte virus OX=302100 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.04 SPYY27 pKa = 9.1 TSRR30 pKa = 11.84 AVAPIVPGTSRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 9.79 RR48 pKa = 11.84 PMYY51 pKa = 9.7 RR52 pKa = 11.84 KK53 pKa = 9.08 PRR55 pKa = 11.84 TYY57 pKa = 11.0 RR58 pKa = 11.84 MYY60 pKa = 10.75 RR61 pKa = 11.84 SPDD64 pKa = 3.29 VPKK67 pKa = 10.81 GCEE70 pKa = 4.39 GPCKK74 pKa = 9.35 VQSYY78 pKa = 6.99 EE79 pKa = 3.67 QRR81 pKa = 11.84 DD82 pKa = 4.05 DD83 pKa = 3.72 VKK85 pKa = 9.5 HH86 pKa = 5.48 TGIVRR91 pKa = 11.84 CVSDD95 pKa = 3.31 VTRR98 pKa = 11.84 GSGITHH104 pKa = 6.35 RR105 pKa = 11.84 VGKK108 pKa = 9.5 RR109 pKa = 11.84 FCVKK113 pKa = 10.3 SIYY116 pKa = 10.1 ILGKK120 pKa = 9.09 IWMDD124 pKa = 3.64 EE125 pKa = 4.09 NIKK128 pKa = 10.06 KK129 pKa = 9.97 QNHH132 pKa = 4.95 TNQVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.77 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 10.07 GAAPMDD153 pKa = 4.26 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.11 PSTATVKK171 pKa = 10.81 NDD173 pKa = 2.84 LRR175 pKa = 11.84 DD176 pKa = 3.47 RR177 pKa = 11.84 YY178 pKa = 9.28 QVLRR182 pKa = 11.84 KK183 pKa = 8.77 FHH185 pKa = 5.93 ATVVGGPSGMKK196 pKa = 9.47 EE197 pKa = 3.54 QALVKK202 pKa = 10.31 RR203 pKa = 11.84 FFRR206 pKa = 11.84 INSHH210 pKa = 3.89 VTYY213 pKa = 10.54 NHH215 pKa = 5.79 QEE217 pKa = 3.49 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 9.86 ATLKK247 pKa = 9.47 IRR249 pKa = 11.84 IYY251 pKa = 10.69 FYY253 pKa = 11.26 DD254 pKa = 3.31 SVGNN258 pKa = 3.81
Molecular weight: 29.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.287
IPC2_protein 9.589
IPC_protein 9.955
Toseland 10.101
ProMoST 9.882
Dawson 10.335
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.628
Grimsley 10.423
Solomon 10.365
Lehninger 10.321
Nozaki 10.131
DTASelect 10.043
Thurlkill 10.189
EMBOSS 10.526
Sillero 10.262
Patrickios 10.058
IPC_peptide 10.365
IPC2_peptide 8.975
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1165
116
379
194.2
22.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.322 ± 0.63
2.232 ± 0.426
4.549 ± 0.541
5.064 ± 0.725
4.378 ± 0.366
4.721 ± 0.425
3.948 ± 0.792
6.009 ± 0.893
5.923 ± 0.457
7.21 ± 0.921
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.367
4.979 ± 0.629
6.438 ± 0.66
5.494 ± 0.755
6.953 ± 1.008
8.412 ± 1.488
5.751 ± 0.921
5.236 ± 1.263
1.288 ± 0.219
3.863 ± 0.569
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here