Termite associated circular virus 2
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1E314|A0A2P1E314_9VIRU Replication-associated protein OS=Termite associated circular virus 2 OX=2108550 PE=3 SV=1
MM1 pKa = 7.68 PAFVCNARR9 pKa = 11.84 YY10 pKa = 8.98 FLITFAQCDD19 pKa = 3.98 ALDD22 pKa = 3.64 PWAVSNHH29 pKa = 5.43 FSTLGAEE36 pKa = 4.41 CIVARR41 pKa = 11.84 EE42 pKa = 4.04 SHH44 pKa = 6.74 ADD46 pKa = 3.43 GGTHH50 pKa = 6.29 LHH52 pKa = 6.48 AFVDD56 pKa = 4.82 FGRR59 pKa = 11.84 KK60 pKa = 8.13 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 PGIFDD70 pKa = 3.79 VAGCHH75 pKa = 6.08 PNISPSRR82 pKa = 11.84 GSPGAGYY89 pKa = 10.44 DD90 pKa = 3.6 YY91 pKa = 10.27 ATKK94 pKa = 10.77 DD95 pKa = 3.21 GDD97 pKa = 3.74 IVAGGLARR105 pKa = 11.84 PSVDD109 pKa = 3.37 AGDD112 pKa = 3.93 GKK114 pKa = 10.78 VDD116 pKa = 3.15 KK117 pKa = 10.01 WSQIISAEE125 pKa = 4.12 DD126 pKa = 3.37 EE127 pKa = 4.1 RR128 pKa = 11.84 TFWDD132 pKa = 4.0 LLEE135 pKa = 4.58 SLDD138 pKa = 4.22 PKK140 pKa = 11.14 ALATNYY146 pKa = 10.69 GNLRR150 pKa = 11.84 KK151 pKa = 9.87 YY152 pKa = 11.13 ADD154 pKa = 3.56 FRR156 pKa = 11.84 YY157 pKa = 9.91 KK158 pKa = 10.54 PEE160 pKa = 4.73 PEE162 pKa = 4.56 TYY164 pKa = 9.31 QHH166 pKa = 6.8 PAGIEE171 pKa = 3.85 FGGGVLPDD179 pKa = 3.47 LVAWRR184 pKa = 11.84 DD185 pKa = 3.49 SSLGASLGGRR195 pKa = 11.84 RR196 pKa = 11.84 NTSSARR202 pKa = 11.84 GPPGALRR209 pKa = 11.84 VYY211 pKa = 11.5 AMLTSLGRR219 pKa = 11.84 SRR221 pKa = 11.84 SLCIYY226 pKa = 10.17 GPTRR230 pKa = 11.84 VGKK233 pKa = 6.01 TTWARR238 pKa = 11.84 SLGMHH243 pKa = 6.43 VYY245 pKa = 9.93 FMGMISGEE253 pKa = 3.8 IAVRR257 pKa = 11.84 DD258 pKa = 3.99 MPTAEE263 pKa = 3.99 YY264 pKa = 10.42 AIFDD268 pKa = 4.19 DD269 pKa = 3.8 MRR271 pKa = 11.84 GGIKK275 pKa = 10.07 FFPAWKK281 pKa = 9.05 EE282 pKa = 3.64 WFGAQQVVTVKK293 pKa = 10.64 KK294 pKa = 10.54 LYY296 pKa = 10.2 RR297 pKa = 11.84 DD298 pKa = 3.48 PLQINWGKK306 pKa = 8.98 PCIWLSNTDD315 pKa = 3.46 PRR317 pKa = 11.84 TGMEE321 pKa = 4.04 MEE323 pKa = 5.17 DD324 pKa = 3.87 IEE326 pKa = 4.4 WLEE329 pKa = 4.19 GNCDD333 pKa = 3.6 FVFMQDD339 pKa = 4.55 SIISHH344 pKa = 6.93 ANTPP348 pKa = 3.53
Molecular weight: 38.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.909
IPC2_protein 5.893
IPC_protein 5.957
Toseland 6.275
ProMoST 6.351
Dawson 6.262
Bjellqvist 6.249
Wikipedia 6.287
Rodwell 6.249
Grimsley 6.402
Solomon 6.275
Lehninger 6.262
Nozaki 6.532
DTASelect 6.722
Thurlkill 6.737
EMBOSS 6.707
Sillero 6.649
Patrickios 4.139
IPC_peptide 6.287
IPC2_peptide 6.605
IPC2.peptide.svr19 6.557
Protein with the highest isoelectric point:
>tr|A0A2P1E314|A0A2P1E314_9VIRU Replication-associated protein OS=Termite associated circular virus 2 OX=2108550 PE=3 SV=1
MM1 pKa = 7.65 AYY3 pKa = 10.05 GRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 LYY9 pKa = 10.45 SSRR12 pKa = 11.84 VKK14 pKa = 10.08 TRR16 pKa = 11.84 TGISGRR22 pKa = 11.84 RR23 pKa = 11.84 GRR25 pKa = 11.84 YY26 pKa = 4.73 STRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 10.21 VKK33 pKa = 9.05 TRR35 pKa = 11.84 PLARR39 pKa = 11.84 RR40 pKa = 11.84 TYY42 pKa = 9.15 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 TTMSRR51 pKa = 11.84 RR52 pKa = 11.84 KK53 pKa = 9.23 ILNISSEE60 pKa = 4.27 KK61 pKa = 10.8 KK62 pKa = 9.85 KK63 pKa = 10.38 DD64 pKa = 3.69 TMLTWTNLQGSVGVEE79 pKa = 3.97 TPGPAKK85 pKa = 10.26 LAPPSPDD92 pKa = 3.0 NLMTEE97 pKa = 4.36 YY98 pKa = 10.67 CVVFSPTHH106 pKa = 5.87 RR107 pKa = 11.84 GATFSSGSHH116 pKa = 5.28 GSKK119 pKa = 10.28 YY120 pKa = 10.36 YY121 pKa = 10.27 KK122 pKa = 9.36 QLRR125 pKa = 11.84 TSSLCYY131 pKa = 8.6 WRR133 pKa = 11.84 GIRR136 pKa = 11.84 EE137 pKa = 4.28 VVTLKK142 pKa = 10.17 TDD144 pKa = 4.01 GPGAWEE150 pKa = 3.64 WRR152 pKa = 11.84 RR153 pKa = 11.84 ICFTMKK159 pKa = 10.9 GEE161 pKa = 4.27 DD162 pKa = 4.24 LYY164 pKa = 11.31 TGDD167 pKa = 4.86 RR168 pKa = 11.84 YY169 pKa = 11.32 SFDD172 pKa = 4.4 LGTSPVARR180 pKa = 11.84 ITSDD184 pKa = 2.62 GMKK187 pKa = 10.03 RR188 pKa = 11.84 AFFSSEE194 pKa = 3.35 NALRR198 pKa = 11.84 TRR200 pKa = 11.84 DD201 pKa = 3.85 AIFDD205 pKa = 3.77 GNRR208 pKa = 11.84 NVDD211 pKa = 3.1 WADD214 pKa = 3.48 VITAKK219 pKa = 10.46 VDD221 pKa = 3.5 TEE223 pKa = 5.21 LITPLYY229 pKa = 9.36 DD230 pKa = 2.9 RR231 pKa = 11.84 RR232 pKa = 11.84 MLIRR236 pKa = 11.84 SGNEE240 pKa = 3.17 AGTVRR245 pKa = 11.84 IKK247 pKa = 10.84 KK248 pKa = 9.31 CWHH251 pKa = 6.27 AMNKK255 pKa = 8.78 NLQYY259 pKa = 11.45 DD260 pKa = 3.51 DD261 pKa = 5.08 DD262 pKa = 4.88 EE263 pKa = 6.21 NGEE266 pKa = 4.45 SMLEE270 pKa = 3.86 RR271 pKa = 11.84 YY272 pKa = 9.72 YY273 pKa = 10.45 STEE276 pKa = 3.87 GKK278 pKa = 9.54 PGMGDD283 pKa = 3.45 YY284 pKa = 11.08 YY285 pKa = 10.43 IVDD288 pKa = 4.39 LFQAVVPQQNVTPTNLYY305 pKa = 10.74 VNIEE309 pKa = 3.92 ATAYY313 pKa = 7.14 WHH315 pKa = 6.3 EE316 pKa = 4.26 RR317 pKa = 3.4
Molecular weight: 36.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.843
IPC2_protein 9.458
IPC_protein 9.78
Toseland 9.867
ProMoST 9.78
Dawson 10.16
Bjellqvist 9.911
Wikipedia 10.394
Rodwell 10.35
Grimsley 10.262
Solomon 10.189
Lehninger 10.131
Nozaki 9.911
DTASelect 9.897
Thurlkill 9.984
EMBOSS 10.306
Sillero 10.087
Patrickios 5.41
IPC_peptide 10.175
IPC2_peptide 8.785
IPC2.peptide.svr19 8.488
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
665
317
348
332.5
37.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.368 ± 1.301
1.654 ± 0.255
6.466 ± 0.511
4.511 ± 0.143
3.91 ± 0.899
9.023 ± 0.942
1.805 ± 0.352
4.662 ± 0.364
4.511 ± 0.552
6.466 ± 0.102
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.008 ± 0.095
3.459 ± 0.417
5.113 ± 0.657
1.955 ± 0.04
9.173 ± 1.621
7.218 ± 0.229
7.068 ± 1.555
5.414 ± 0.172
2.556 ± 0.226
4.662 ± 0.864
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here