Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Machlomovirus; Maize chlorotic mottle virus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P11640|RDRP_MCMVK RNA-directed RNA polymerase OS=Maize chlorotic mottle virus (isolate United States/Kansas/1987) OX=882210 GN=ORF2 PE=3 SV=3
MM1 pKa = 8.05PSPCTYY7 pKa = 11.18GDD9 pKa = 3.25PTFNSRR15 pKa = 11.84ILEE18 pKa = 4.1AVVADD23 pKa = 3.77VLGGTEE29 pKa = 4.53DD30 pKa = 3.79DD31 pKa = 4.56GGPSLEE37 pKa = 4.3EE38 pKa = 3.71WFDD41 pKa = 3.64AQTLSDD47 pKa = 4.07YY48 pKa = 8.99TNCATDD54 pKa = 3.58PPMATVHH61 pKa = 5.35TRR63 pKa = 11.84EE64 pKa = 4.08NDD66 pKa = 3.14IKK68 pKa = 11.02SWTEE72 pKa = 3.4LSEE75 pKa = 4.08NFPDD79 pKa = 3.92LVRR82 pKa = 11.84YY83 pKa = 9.24PEE85 pKa = 4.28SLVEE89 pKa = 4.02TLLDD93 pKa = 3.71TEE95 pKa = 4.91HH96 pKa = 6.99EE97 pKa = 4.53CGHH100 pKa = 6.58FYY102 pKa = 10.79DD103 pKa = 5.49APDD106 pKa = 3.42SFQISVTAMFTDD118 pKa = 3.6RR119 pKa = 11.84CKK121 pKa = 11.04CQFCDD126 pKa = 3.65PNFQARR132 pKa = 11.84SLSRR136 pKa = 11.84ALLGPLPEE144 pKa = 6.34SGDD147 pKa = 3.49DD148 pKa = 4.08AEE150 pKa = 4.58WMEE153 pKa = 4.72QAYY156 pKa = 8.22TPDD159 pKa = 3.61AEE161 pKa = 4.76LFVNEE166 pKa = 4.67PTDD169 pKa = 4.38DD170 pKa = 5.42PIPTTDD176 pKa = 4.04CKK178 pKa = 10.94RR179 pKa = 11.84PIQPTWSVDD188 pKa = 3.6VYY190 pKa = 11.15SKK192 pKa = 10.84QVDD195 pKa = 4.09SDD197 pKa = 3.37WGLSDD202 pKa = 3.37STNVTVCSASSSLLPAGRR220 pKa = 11.84GGIYY224 pKa = 9.73FMCPRR229 pKa = 11.84PEE231 pKa = 5.09GIEE234 pKa = 4.06RR235 pKa = 11.84VCLQVGAKK243 pKa = 8.97IRR245 pKa = 11.84SGAPNSGTITNLPGGTGYY263 pKa = 8.34GTEE266 pKa = 4.05WSDD269 pKa = 5.29DD270 pKa = 3.78GYY272 pKa = 9.67EE273 pKa = 4.35TQWSDD278 pKa = 3.29GPYY281 pKa = 9.8SIPSGLSDD289 pKa = 3.3

Molecular weight: 31.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P11642|CAPSD_MCMVK Capsid protein OS=Maize chlorotic mottle virus (isolate United States/Kansas/1987) OX=882210 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.39AASSRR6 pKa = 11.84STRR9 pKa = 11.84GRR11 pKa = 11.84KK12 pKa = 7.81QRR14 pKa = 11.84GRR16 pKa = 11.84SVEE19 pKa = 4.0AKK21 pKa = 10.19SRR23 pKa = 11.84AIRR26 pKa = 11.84ANPPVPRR33 pKa = 11.84PNPQRR38 pKa = 11.84NRR40 pKa = 11.84PPPAGTTCSMSEE52 pKa = 3.57ILLAVSATTADD63 pKa = 3.85QILEE67 pKa = 4.13IPVCAGIDD75 pKa = 3.95FPAGTSPRR83 pKa = 11.84YY84 pKa = 9.62IGAAKK89 pKa = 9.45WLAAQSQMWNTIVFNSVRR107 pKa = 11.84ITWEE111 pKa = 3.65TFTADD116 pKa = 3.25TTSGYY121 pKa = 10.62ISMAFLSDD129 pKa = 3.68YY130 pKa = 9.81MLSIPTGVEE139 pKa = 3.6DD140 pKa = 3.57VARR143 pKa = 11.84IVPSATIALKK153 pKa = 10.74NRR155 pKa = 11.84GPSIVMPQNRR165 pKa = 11.84TVFRR169 pKa = 11.84CIQAGQFAALGSAADD184 pKa = 3.64KK185 pKa = 11.04QMYY188 pKa = 9.84SPGRR192 pKa = 11.84FIVAIPKK199 pKa = 10.33ASATQAVGQIKK210 pKa = 9.98ISYY213 pKa = 7.72SVSYY217 pKa = 10.16RR218 pKa = 11.84GAAILQPALVPGPGLANHH236 pKa = 7.38

Molecular weight: 25.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1833

64

965

366.6

41.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.819 ± 1.321

2.4 ± 0.489

4.637 ± 1.049

5.346 ± 0.751

3.873 ± 0.319

6.219 ± 0.413

2.182 ± 0.486

5.074 ± 0.846

3.983 ± 0.701

8.62 ± 0.999

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.381

3.819 ± 0.22

6.601 ± 0.76

5.128 ± 0.822

7.147 ± 0.873

7.529 ± 0.937

6.874 ± 0.359

6.274 ± 0.227

2.073 ± 0.171

3.001 ± 0.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski