Arthrobacter phage vB_ArtM-ArV1
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7HAK3|A0A0A7HAK3_9CAUD Major capsid protein OS=Arthrobacter phage vB_ArtM-ArV1 OX=1566993 GN=ArV1_009 PE=4 SV=1
MM1 pKa = 7.26 AQLTFEE7 pKa = 4.4 EE8 pKa = 3.99 ARR10 pKa = 11.84 AAVRR14 pKa = 11.84 AQNQPIWEE22 pKa = 4.83 DD23 pKa = 2.88 NDD25 pKa = 4.04 TPGEE29 pKa = 4.12 YY30 pKa = 9.17 MVAGYY35 pKa = 9.93 GWEE38 pKa = 3.94 NDD40 pKa = 3.05 EE41 pKa = 5.53 SYY43 pKa = 11.63 LLVEE47 pKa = 4.83 GARR50 pKa = 11.84 EE51 pKa = 3.89 YY52 pKa = 11.51 LEE54 pKa = 5.85 DD55 pKa = 4.01 GLFEE59 pKa = 4.41 YY60 pKa = 11.15 VVMDD64 pKa = 4.6 APAVFVDD71 pKa = 4.18 KK72 pKa = 10.3 EE73 pKa = 4.45 TGTVALAEE81 pKa = 4.18 FLTVFEE87 pKa = 5.66 VIQSMTPVPGHH98 pKa = 6.28 EE99 pKa = 4.88 PPTDD103 pKa = 4.2 PDD105 pKa = 3.7 DD106 pKa = 5.81 DD107 pKa = 4.1 EE108 pKa = 4.73
Molecular weight: 12.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.824
IPC2_protein 3.63
IPC_protein 3.567
Toseland 3.376
ProMoST 3.694
Dawson 3.541
Bjellqvist 3.757
Wikipedia 3.465
Rodwell 3.401
Grimsley 3.3
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.821
Thurlkill 3.427
EMBOSS 3.478
Sillero 3.681
Patrickios 1.774
IPC_peptide 3.516
IPC2_peptide 3.656
IPC2.peptide.svr19 3.692
Protein with the highest isoelectric point:
>tr|A0A0A7HBU5|A0A0A7HBU5_9CAUD Uncharacterized protein OS=Arthrobacter phage vB_ArtM-ArV1 OX=1566993 GN=ArV1_048 PE=4 SV=1
MM1 pKa = 7.81 AGRR4 pKa = 11.84 EE5 pKa = 3.98 RR6 pKa = 11.84 HH7 pKa = 6.09 PLPGGPPRR15 pKa = 11.84 QMPHH19 pKa = 5.16 MRR21 pKa = 11.84 RR22 pKa = 11.84 GPRR25 pKa = 11.84 QRR27 pKa = 11.84 LPRR30 pKa = 11.84 PLEE33 pKa = 3.85 RR34 pKa = 11.84 RR35 pKa = 11.84 NGHH38 pKa = 5.22 TPPRR42 pKa = 11.84 PHH44 pKa = 5.54 QRR46 pKa = 11.84 SRR48 pKa = 11.84 GSPTNQPTGKK58 pKa = 10.22 DD59 pKa = 3.18 MPLLHH64 pKa = 7.06 GKK66 pKa = 8.77 PGHH69 pKa = 5.81 AVRR72 pKa = 11.84 RR73 pKa = 11.84 PKK75 pKa = 9.78 WPNHH79 pKa = 4.96 CRR81 pKa = 11.84 FPRR84 pKa = 11.84 IPRR87 pKa = 11.84 GIRR90 pKa = 11.84 MTTGMTNNQAALLAATTANANRR112 pKa = 11.84 LVTNRR117 pKa = 11.84 EE118 pKa = 3.49 IVQATMSFKK127 pKa = 10.82 EE128 pKa = 4.79 LLDD131 pKa = 3.9 TLDD134 pKa = 4.03 KK135 pKa = 11.18 QDD137 pKa = 3.55 ATTQAEE143 pKa = 4.16 AEE145 pKa = 4.27 KK146 pKa = 11.14 AEE148 pKa = 4.91 DD149 pKa = 3.99 YY150 pKa = 10.58 QALPAYY156 pKa = 7.32 CQPHH160 pKa = 6.12 LKK162 pKa = 9.79 EE163 pKa = 4.26 VYY165 pKa = 10.61 ADD167 pKa = 3.8 FWGQGVPKK175 pKa = 10.4 ARR177 pKa = 11.84 ALQPGEE183 pKa = 4.31 HH184 pKa = 6.3 CTGNPFSPTQCEE196 pKa = 4.14 NSPAPQPNEE205 pKa = 3.69 EE206 pKa = 4.35 GTNQQ210 pKa = 3.19
Molecular weight: 23.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.355
IPC_protein 9.794
Toseland 10.423
ProMoST 10.058
Dawson 10.526
Bjellqvist 10.218
Wikipedia 10.701
Rodwell 10.745
Grimsley 10.57
Solomon 10.613
Lehninger 10.584
Nozaki 10.438
DTASelect 10.204
Thurlkill 10.423
EMBOSS 10.818
Sillero 10.467
Patrickios 10.482
IPC_peptide 10.613
IPC2_peptide 9.311
IPC2.peptide.svr19 8.447
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
101
0
101
22761
67
1177
225.4
24.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.915 ± 0.343
0.861 ± 0.124
5.883 ± 0.166
6.059 ± 0.302
2.834 ± 0.163
8.352 ± 0.361
2.289 ± 0.177
4.016 ± 0.18
4.402 ± 0.234
7.465 ± 0.213
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.478 ± 0.098
3.849 ± 0.151
6.581 ± 0.257
3.423 ± 0.161
5.896 ± 0.266
4.89 ± 0.209
7.043 ± 0.234
7.473 ± 0.264
1.836 ± 0.136
2.456 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here