Kangiella geojedonensis
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2207 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6RCU7|A0A0F6RCU7_9GAMM M6 family metalloprotease domain protein OS=Kangiella geojedonensis OX=914150 GN=TQ33_1379 PE=4 SV=1
MM1 pKa = 6.95 VVSKK5 pKa = 10.74 AVYY8 pKa = 9.84 KK9 pKa = 10.8 GFILLMLLLTCVRR22 pKa = 11.84 VEE24 pKa = 4.03 SATVEE29 pKa = 4.43 FQVNWPEE36 pKa = 3.54 WSQEE40 pKa = 3.74 NRR42 pKa = 11.84 IEE44 pKa = 4.38 FYY46 pKa = 9.99 NTAVDD51 pKa = 3.62 VADD54 pKa = 4.82 RR55 pKa = 11.84 DD56 pKa = 3.81 GDD58 pKa = 4.05 GNTTEE63 pKa = 5.09 IINSNLVTTRR73 pKa = 11.84 YY74 pKa = 10.04 GGEE77 pKa = 3.84 NAICVPGFCTLPAVANTSFSQNYY100 pKa = 6.95 TFNNVPSGNYY110 pKa = 9.48 RR111 pKa = 11.84 IVMYY115 pKa = 10.73 DD116 pKa = 3.46 DD117 pKa = 6.43 FGDD120 pKa = 3.49 GWNGGGTLTVLVNGTNIGTVTTTTSVTSTNVFTISDD156 pKa = 4.17 TPTGTTAQTCSDD168 pKa = 4.08 ANLTNAWSNLGASPQATAGAIPVSISTTDD197 pKa = 3.12 TGPAGWVFSSDD208 pKa = 2.98 TMNTINAWSSAAVQGRR224 pKa = 11.84 TSLGVLFEE232 pKa = 4.38 WDD234 pKa = 3.81 PTPGANGFEE243 pKa = 4.27 PVYY246 pKa = 10.52 QATNSDD252 pKa = 3.56 GVVNDD257 pKa = 4.01 NDD259 pKa = 4.19 PGGEE263 pKa = 3.97 GTMDD267 pKa = 2.93 ISFGGRR273 pKa = 11.84 TVNNAIVHH281 pKa = 6.24 IDD283 pKa = 3.26 RR284 pKa = 11.84 VGGIGAGVTNSSRR297 pKa = 11.84 WGINTAGVTLGRR309 pKa = 11.84 LAGVGHH315 pKa = 6.89 FATYY319 pKa = 9.96 TNNTFQRR326 pKa = 11.84 DD327 pKa = 3.93 MLVVSANTEE336 pKa = 4.06 SSTNTATGTAAGSVVYY352 pKa = 10.29 QGEE355 pKa = 4.42 YY356 pKa = 9.17 STLSLGLDD364 pKa = 4.24 GIGLEE369 pKa = 4.48 GAGGDD374 pKa = 3.67 GMEE377 pKa = 4.58 MVVCVPQADD386 pKa = 3.84 LSLTKK391 pKa = 10.26 TVDD394 pKa = 3.18 NSNPNVGEE402 pKa = 4.0 NVTYY406 pKa = 10.24 TLTVTNNGPDD416 pKa = 3.48 ATNDD420 pKa = 3.02 IQVRR424 pKa = 11.84 DD425 pKa = 4.18 LLPAEE430 pKa = 4.7 LDD432 pKa = 3.65 FVSATEE438 pKa = 4.24 SQGSFASGSGIWTVGTLANGASATLDD464 pKa = 3.02 IVVSPNTAGVITNEE478 pKa = 3.95 AEE480 pKa = 4.09 IINADD485 pKa = 4.41 RR486 pKa = 11.84 IDD488 pKa = 4.16 PDD490 pKa = 3.68 SEE492 pKa = 4.12 FRR494 pKa = 11.84 SGLATDD500 pKa = 4.91 DD501 pKa = 4.38 LADD504 pKa = 5.51 GIADD508 pKa = 4.05 DD509 pKa = 5.78 DD510 pKa = 4.3 EE511 pKa = 6.79 ASVDD515 pKa = 3.43 INAGVVDD522 pKa = 3.9 VSVTKK527 pKa = 10.7 SSSNDD532 pKa = 3.73 FIPGGTDD539 pKa = 2.74 TYY541 pKa = 10.94 TIIVSNAGPSDD552 pKa = 4.42 AIGLLVSDD560 pKa = 4.86 NLPSGVTLSGSWSCTPSAAGSSCNSASGGAAGGTSVSVTVNVAAGEE606 pKa = 4.21 QVTITVPVEE615 pKa = 3.82 YY616 pKa = 10.52 SADD619 pKa = 3.59 PTDD622 pKa = 3.67 YY623 pKa = 11.62
Molecular weight: 63.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.427
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.783
Patrickios 1.011
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A0F6RAX0|A0A0F6RAX0_9GAMM 4-hydroxybenzoate octaprenyltransferase OS=Kangiella geojedonensis OX=914150 GN=ubiA PE=3 SV=1
MM1 pKa = 7.25 ATINQLVRR9 pKa = 11.84 KK10 pKa = 8.95 PRR12 pKa = 11.84 VKK14 pKa = 9.97 QVKK17 pKa = 8.11 KK18 pKa = 8.73 TNVPALEE25 pKa = 4.4 ACPQKK30 pKa = 10.67 RR31 pKa = 11.84 GVCTRR36 pKa = 11.84 VYY38 pKa = 7.93 TTTPKK43 pKa = 10.54 KK44 pKa = 10.26 PNSALRR50 pKa = 11.84 KK51 pKa = 7.21 VARR54 pKa = 11.84 VRR56 pKa = 11.84 LTNGYY61 pKa = 8.94 EE62 pKa = 3.76 VSSYY66 pKa = 10.86 IGGEE70 pKa = 3.91 GHH72 pKa = 6.6 NLQEE76 pKa = 4.28 HH77 pKa = 5.6 SVVLIRR83 pKa = 11.84 GGRR86 pKa = 11.84 VKK88 pKa = 10.7 DD89 pKa = 3.73 LPGVRR94 pKa = 11.84 YY95 pKa = 9.07 HH96 pKa = 6.62 CVRR99 pKa = 11.84 GTLDD103 pKa = 3.37 LAGVKK108 pKa = 9.49 DD109 pKa = 4.14 RR110 pKa = 11.84 KK111 pKa = 9.86 QGRR114 pKa = 11.84 SKK116 pKa = 11.18 YY117 pKa = 9.02 GAKK120 pKa = 9.93 RR121 pKa = 11.84 PKK123 pKa = 10.33 GG124 pKa = 3.5
Molecular weight: 13.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.984
IPC_protein 10.76
Toseland 10.994
ProMoST 10.687
Dawson 11.067
Bjellqvist 10.774
Wikipedia 11.272
Rodwell 11.389
Grimsley 11.111
Solomon 11.213
Lehninger 11.169
Nozaki 10.979
DTASelect 10.774
Thurlkill 10.979
EMBOSS 11.403
Sillero 11.008
Patrickios 11.111
IPC_peptide 11.213
IPC2_peptide 9.78
IPC2.peptide.svr19 8.317
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2207
0
2207
737553
50
8155
334.2
37.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.116 ± 0.065
0.869 ± 0.021
6.073 ± 0.087
6.873 ± 0.061
4.066 ± 0.035
6.979 ± 0.057
2.198 ± 0.031
6.317 ± 0.041
5.893 ± 0.082
9.917 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.448 ± 0.035
4.283 ± 0.042
3.867 ± 0.026
4.361 ± 0.049
4.353 ± 0.057
6.698 ± 0.054
5.341 ± 0.171
6.927 ± 0.047
1.211 ± 0.023
3.212 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here