Telfairia golden mosaic virus
Average proteome isoelectric point is 8.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KVT3|A0A191KVT3_9GEMI Replication-associated protein OS=Telfairia golden mosaic virus OX=2169737 PE=3 SV=1
MM1 pKa = 7.2 HH2 pKa = 7.46 AEE4 pKa = 4.14 SYY6 pKa = 9.79 SAYY9 pKa = 10.56 VLSLYY14 pKa = 8.87 TWSPYY19 pKa = 9.01 FHH21 pKa = 6.87 YY22 pKa = 10.99 RR23 pKa = 11.84 NMWDD27 pKa = 3.29 PLLNEE32 pKa = 4.55 FPEE35 pKa = 4.66 SVHH38 pKa = 6.1 GFRR41 pKa = 11.84 SMLAVKK47 pKa = 9.84 YY48 pKa = 9.53 LQAVEE53 pKa = 3.99 QTYY56 pKa = 10.31 EE57 pKa = 4.23 PNTLGHH63 pKa = 6.99 DD64 pKa = 4.89 LIRR67 pKa = 11.84 DD68 pKa = 4.46 LICVIRR74 pKa = 11.84 AKK76 pKa = 10.71 DD77 pKa = 3.54 YY78 pKa = 10.69 VEE80 pKa = 3.48 ATRR83 pKa = 11.84 RR84 pKa = 11.84 YY85 pKa = 8.99 NYY87 pKa = 9.78 FHH89 pKa = 7.18 ARR91 pKa = 11.84 LEE93 pKa = 4.48 GASKK97 pKa = 11.25 AEE99 pKa = 3.98 LRR101 pKa = 11.84 QPIYY105 pKa = 10.44 QPCCCPHH112 pKa = 6.7 CPRR115 pKa = 11.84 HH116 pKa = 5.4 KK117 pKa = 9.58 QTTIMDD123 pKa = 3.89 VSSQLPKK130 pKa = 10.74 AQDD133 pKa = 3.37 VQDD136 pKa = 3.89 VQKK139 pKa = 11.2 SS140 pKa = 3.38
Molecular weight: 16.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.54
IPC2_protein 6.478
IPC_protein 6.693
Toseland 6.707
ProMoST 6.971
Dawson 7.176
Bjellqvist 7.029
Wikipedia 7.146
Rodwell 7.161
Grimsley 6.81
Solomon 7.22
Lehninger 7.234
Nozaki 7.38
DTASelect 7.468
Thurlkill 7.497
EMBOSS 7.527
Sillero 7.585
Patrickios 2.181
IPC_peptide 7.234
IPC2_peptide 7.249
IPC2.peptide.svr19 7.145
Protein with the highest isoelectric point:
>tr|A0A191KVT2|A0A191KVT2_9GEMI Protein V2 OS=Telfairia golden mosaic virus OX=2169737 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.95 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.03 SPYY27 pKa = 7.84 TNRR30 pKa = 11.84 VAVPIVQGTSKK41 pKa = 10.21 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 9.41 RR48 pKa = 11.84 PSYY51 pKa = 9.78 RR52 pKa = 11.84 KK53 pKa = 8.69 PRR55 pKa = 11.84 MYY57 pKa = 10.91 RR58 pKa = 11.84 MYY60 pKa = 10.44 RR61 pKa = 11.84 SPDD64 pKa = 3.21 VPRR67 pKa = 11.84 GCEE70 pKa = 4.4 GPCKK74 pKa = 9.97 IQSFDD79 pKa = 3.31 QRR81 pKa = 11.84 DD82 pKa = 4.22 SVVHH86 pKa = 5.94 TGNVRR91 pKa = 11.84 CLSDD95 pKa = 3.23 VTRR98 pKa = 11.84 GGGLTHH104 pKa = 6.43 RR105 pKa = 11.84 TGKK108 pKa = 10.06 RR109 pKa = 11.84 FCIKK113 pKa = 10.23 SIYY116 pKa = 10.05 VLGKK120 pKa = 8.9 VWMDD124 pKa = 3.42 EE125 pKa = 3.95 NVKK128 pKa = 10.46 KK129 pKa = 10.93 SNHH132 pKa = 5.83 TNTCMFWLVRR142 pKa = 11.84 DD143 pKa = 3.64 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 10.9 GNSPQDD153 pKa = 3.31 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.12 PSTATVMNDD173 pKa = 2.37 KK174 pKa = 10.42 RR175 pKa = 11.84 DD176 pKa = 3.49 RR177 pKa = 11.84 YY178 pKa = 9.22 QVLRR182 pKa = 11.84 RR183 pKa = 11.84 FQVTVTGGPSGCKK196 pKa = 8.84 EE197 pKa = 3.5 AAIVKK202 pKa = 10.02 RR203 pKa = 11.84 FFRR206 pKa = 11.84 LNHH209 pKa = 5.74 HH210 pKa = 5.45 VTYY213 pKa = 10.8 NHH215 pKa = 6.3 QEE217 pKa = 3.75 GAKK220 pKa = 10.15 YY221 pKa = 10.3 EE222 pKa = 4.14 NHH224 pKa = 6.42 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 10.67 ASLKK247 pKa = 8.7 IRR249 pKa = 11.84 MYY251 pKa = 10.73 FYY253 pKa = 11.22 DD254 pKa = 3.51 SVLNN258 pKa = 4.02
Molecular weight: 29.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.56
IPC_protein 9.94
Toseland 10.058
ProMoST 9.853
Dawson 10.306
Bjellqvist 10.043
Wikipedia 10.511
Rodwell 10.57
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.131
DTASelect 10.028
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.233
Patrickios 9.882
IPC_peptide 10.335
IPC2_peptide 9.092
IPC2.peptide.svr19 8.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1624
96
361
203.0
23.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.542 ± 0.473
2.032 ± 0.327
5.296 ± 0.354
4.433 ± 0.659
4.495 ± 0.44
4.988 ± 0.496
3.633 ± 0.311
4.803 ± 0.527
5.419 ± 0.494
7.697 ± 0.577
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.34 ± 0.483
5.419 ± 0.413
5.542 ± 0.293
3.571 ± 0.46
7.82 ± 0.858
8.805 ± 0.881
6.281 ± 0.567
6.589 ± 0.805
1.293 ± 0.135
4.002 ± 0.748
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here