Moumouvirus goulette
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 970 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1PHN4|M1PHN4_9VIRU Repeat protein OS=Moumouvirus goulette OX=1247379 GN=glt_00793 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 5.29 IDD4 pKa = 3.21 ISKK7 pKa = 10.93 YY8 pKa = 10.59 DD9 pKa = 3.77 DD10 pKa = 4.34 CDD12 pKa = 3.51 LVTLFEE18 pKa = 4.03 QVIIEE23 pKa = 4.09 MQTRR27 pKa = 11.84 YY28 pKa = 10.41 INIYY32 pKa = 9.91 KK33 pKa = 9.62 YY34 pKa = 10.36 IPSEE38 pKa = 3.95 IKK40 pKa = 10.25 NDD42 pKa = 3.58 SSKK45 pKa = 9.84 EE46 pKa = 4.02 TLNDD50 pKa = 3.01 NNYY53 pKa = 9.97 NEE55 pKa = 4.32 SLGYY59 pKa = 9.54 YY60 pKa = 9.83
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.745
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.884
Rodwell 3.783
Grimsley 3.668
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.266
Thurlkill 3.808
EMBOSS 3.884
Sillero 4.062
Patrickios 0.477
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.979
Protein with the highest isoelectric point:
>tr|M1NNF5|M1NNF5_9VIRU Uncharacterized protein OS=Moumouvirus goulette OX=1247379 GN=glt_00785 PE=4 SV=1
MM1 pKa = 7.23 KK2 pKa = 10.27 QKK4 pKa = 10.05 QQFKK8 pKa = 10.68 QNQQPRR14 pKa = 11.84 QTLNKK19 pKa = 9.5 NDD21 pKa = 3.9 EE22 pKa = 4.38 PNFTVVKK29 pKa = 10.5 NKK31 pKa = 10.68 AIDD34 pKa = 3.86 LLVDD38 pKa = 3.41 EE39 pKa = 5.35 LVSLRR44 pKa = 11.84 CASDD48 pKa = 3.3 VTQRR52 pKa = 11.84 GPARR56 pKa = 11.84 HH57 pKa = 6.25 CAAIFIEE64 pKa = 4.41 KK65 pKa = 9.85 NQSCFKK71 pKa = 9.97 PTVNWYY77 pKa = 8.53 QSRR80 pKa = 11.84 EE81 pKa = 4.06 LLPEE85 pKa = 3.96 EE86 pKa = 3.7 ARR88 pKa = 11.84 KK89 pKa = 9.69 RR90 pKa = 11.84 SRR92 pKa = 11.84 KK93 pKa = 7.72 KK94 pKa = 9.5 QRR96 pKa = 11.84 EE97 pKa = 3.51 RR98 pKa = 11.84 RR99 pKa = 11.84 YY100 pKa = 10.45 RR101 pKa = 11.84 PISTKK106 pKa = 10.75
Molecular weight: 12.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.443
IPC_protein 9.56
Toseland 10.57
ProMoST 10.116
Dawson 10.643
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 11.125
Grimsley 10.657
Solomon 10.687
Lehninger 10.687
Nozaki 10.555
DTASelect 10.233
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.555
Patrickios 10.877
IPC_peptide 10.701
IPC2_peptide 8.99
IPC2.peptide.svr19 8.532
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
970
0
970
304240
32
2912
313.6
36.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.907 ± 0.07
1.849 ± 0.055
6.264 ± 0.077
5.63 ± 0.079
4.763 ± 0.065
4.629 ± 0.191
2.001 ± 0.046
10.662 ± 0.104
9.215 ± 0.128
8.44 ± 0.088
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.048 ± 0.04
9.925 ± 0.132
3.218 ± 0.066
2.958 ± 0.062
2.965 ± 0.07
6.628 ± 0.085
4.882 ± 0.082
4.382 ± 0.06
0.835 ± 0.027
5.799 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here