Aeromicrobium sp. IC_218

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; unclassified Aeromicrobium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1AK93|A0A4R1AK93_9ACTN DMT family transporter OS=Aeromicrobium sp. IC_218 OX=2545468 GN=E0W78_15825 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 6.23PGLLDD7 pKa = 4.17ILVCPQCRR15 pKa = 11.84STLFVDD21 pKa = 4.17DD22 pKa = 4.98DD23 pKa = 4.67RR24 pKa = 11.84SEE26 pKa = 4.29LVCNACSLAYY36 pKa = 9.03PVRR39 pKa = 11.84DD40 pKa = 5.42DD41 pKa = 4.08IPVMLIDD48 pKa = 4.66EE49 pKa = 4.71ARR51 pKa = 11.84QLDD54 pKa = 3.73

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1AS74|A0A4R1AS74_9ACTN Zinc ABC transporter substrate-binding protein OS=Aeromicrobium sp. IC_218 OX=2545468 GN=E0W78_03875 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.56KK16 pKa = 9.27HH17 pKa = 5.36RR18 pKa = 11.84KK19 pKa = 5.5MLKK22 pKa = 7.41RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3175

0

3175

1021998

33

3025

321.9

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.241 ± 0.068

0.628 ± 0.011

6.655 ± 0.037

5.764 ± 0.041

2.687 ± 0.021

9.104 ± 0.037

2.168 ± 0.023

2.977 ± 0.034

1.967 ± 0.034

10.459 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.777 ± 0.015

1.606 ± 0.023

5.439 ± 0.031

2.801 ± 0.022

7.814 ± 0.05

5.153 ± 0.029

6.158 ± 0.043

10.233 ± 0.043

1.479 ± 0.017

1.89 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski