Amino acid dipepetide frequency for Enterobacter phage vB_EclM_CIP9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.846AlaAla: 5.846 ± 0.419
0.623AlaCys: 0.623 ± 0.108
4.27AlaAsp: 4.27 ± 0.3
5.076AlaGlu: 5.076 ± 0.293
2.584AlaPhe: 2.584 ± 0.209
5.021AlaGly: 5.021 ± 0.481
1.264AlaHis: 1.264 ± 0.172
4.581AlaIle: 4.581 ± 0.279
5.901AlaLys: 5.901 ± 0.39
6.579AlaLeu: 6.579 ± 0.435
1.961AlaMet: 1.961 ± 0.188
3.335AlaAsn: 3.335 ± 0.237
2.657AlaPro: 2.657 ± 0.216
2.694AlaGln: 2.694 ± 0.262
3.317AlaArg: 3.317 ± 0.228
4.196AlaSer: 4.196 ± 0.305
4.398AlaThr: 4.398 ± 0.458
4.765AlaVal: 4.765 ± 0.319
0.898AlaTrp: 0.898 ± 0.139
2.694AlaTyr: 2.694 ± 0.23
0.0AlaXaa: 0.0 ± 0.0
Cys
0.715CysAla: 0.715 ± 0.12
0.128CysCys: 0.128 ± 0.05
0.806CysAsp: 0.806 ± 0.106
0.751CysGlu: 0.751 ± 0.114
0.403CysPhe: 0.403 ± 0.092
0.623CysGly: 0.623 ± 0.134
0.202CysHis: 0.202 ± 0.059
0.568CysIle: 0.568 ± 0.107
0.66CysLys: 0.66 ± 0.111
0.678CysLeu: 0.678 ± 0.105
0.403CysMet: 0.403 ± 0.085
0.568CysAsn: 0.568 ± 0.113
0.44CysPro: 0.44 ± 0.103
0.476CysGln: 0.476 ± 0.112
0.586CysArg: 0.586 ± 0.106
0.843CysSer: 0.843 ± 0.144
0.788CysThr: 0.788 ± 0.138
0.678CysVal: 0.678 ± 0.12
0.11CysTrp: 0.11 ± 0.041
0.476CysTyr: 0.476 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.6AspAla: 4.6 ± 0.325
0.513AspCys: 0.513 ± 0.095
3.995AspAsp: 3.995 ± 0.368
4.838AspGlu: 4.838 ± 0.347
3.573AspPhe: 3.573 ± 0.256
4.893AspGly: 4.893 ± 0.32
1.045AspHis: 1.045 ± 0.118
4.38AspIle: 4.38 ± 0.288
3.977AspLys: 3.977 ± 0.256
4.874AspLeu: 4.874 ± 0.305
2.071AspMet: 2.071 ± 0.171
3.189AspAsn: 3.189 ± 0.249
2.511AspPro: 2.511 ± 0.249
1.887AspGln: 1.887 ± 0.157
2.254AspArg: 2.254 ± 0.189
3.665AspSer: 3.665 ± 0.248
3.28AspThr: 3.28 ± 0.231
4.215AspVal: 4.215 ± 0.266
1.246AspTrp: 1.246 ± 0.139
2.987AspTyr: 2.987 ± 0.287
0.0AspXaa: 0.0 ± 0.0
Glu
5.901GluAla: 5.901 ± 0.392
0.935GluCys: 0.935 ± 0.15
4.141GluAsp: 4.141 ± 0.295
5.168GluGlu: 5.168 ± 0.336
3.317GluPhe: 3.317 ± 0.245
4.068GluGly: 4.068 ± 0.292
1.246GluHis: 1.246 ± 0.159
5.754GluIle: 5.754 ± 0.294
4.361GluLys: 4.361 ± 0.321
6.835GluLeu: 6.835 ± 0.417
2.364GluMet: 2.364 ± 0.231
3.39GluAsn: 3.39 ± 0.24
2.016GluPro: 2.016 ± 0.201
2.785GluGln: 2.785 ± 0.189
3.189GluArg: 3.189 ± 0.222
3.537GluSer: 3.537 ± 0.271
3.83GluThr: 3.83 ± 0.243
5.296GluVal: 5.296 ± 0.296
0.99GluTrp: 0.99 ± 0.116
3.097GluTyr: 3.097 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
3.225PheAla: 3.225 ± 0.253
0.55PheCys: 0.55 ± 0.097
3.189PheAsp: 3.189 ± 0.26
3.573PheGlu: 3.573 ± 0.28
1.503PhePhe: 1.503 ± 0.181
3.06PheGly: 3.06 ± 0.252
0.715PheHis: 0.715 ± 0.098
2.895PheIle: 2.895 ± 0.225
4.141PheLys: 4.141 ± 0.27
2.437PheLeu: 2.437 ± 0.232
1.466PheMet: 1.466 ± 0.152
2.84PheAsn: 2.84 ± 0.249
1.283PhePro: 1.283 ± 0.132
1.521PheGln: 1.521 ± 0.194
1.942PheArg: 1.942 ± 0.171
2.456PheSer: 2.456 ± 0.188
2.291PheThr: 2.291 ± 0.186
3.134PheVal: 3.134 ± 0.216
0.861PheTrp: 0.861 ± 0.138
1.594PheTyr: 1.594 ± 0.127
0.0PheXaa: 0.0 ± 0.0
Gly
3.903GlyAla: 3.903 ± 0.415
0.66GlyCys: 0.66 ± 0.104
4.141GlyAsp: 4.141 ± 0.301
4.215GlyGlu: 4.215 ± 0.269
3.207GlyPhe: 3.207 ± 0.256
3.903GlyGly: 3.903 ± 0.409
1.118GlyHis: 1.118 ± 0.146
4.086GlyIle: 4.086 ± 0.208
4.288GlyLys: 4.288 ± 0.342
5.058GlyLeu: 5.058 ± 0.345
1.906GlyMet: 1.906 ± 0.199
3.335GlyAsn: 3.335 ± 0.308
1.997GlyPro: 1.997 ± 0.19
2.162GlyGln: 2.162 ± 0.194
3.097GlyArg: 3.097 ± 0.233
4.416GlySer: 4.416 ± 0.265
4.013GlyThr: 4.013 ± 0.308
4.05GlyVal: 4.05 ± 0.261
1.136GlyTrp: 1.136 ± 0.133
2.877GlyTyr: 2.877 ± 0.229
0.0GlyXaa: 0.0 ± 0.0
His
1.1HisAla: 1.1 ± 0.143
0.367HisCys: 0.367 ± 0.091
1.008HisAsp: 1.008 ± 0.139
0.99HisGlu: 0.99 ± 0.137
0.825HisPhe: 0.825 ± 0.129
1.283HisGly: 1.283 ± 0.143
0.403HisHis: 0.403 ± 0.101
1.356HisIle: 1.356 ± 0.181
1.283HisLys: 1.283 ± 0.139
1.613HisLeu: 1.613 ± 0.157
0.458HisMet: 0.458 ± 0.087
0.88HisAsn: 0.88 ± 0.12
0.953HisPro: 0.953 ± 0.138
0.623HisGln: 0.623 ± 0.118
0.733HisArg: 0.733 ± 0.13
1.191HisSer: 1.191 ± 0.144
0.678HisThr: 0.678 ± 0.117
1.374HisVal: 1.374 ± 0.15
0.183HisTrp: 0.183 ± 0.055
0.88HisTyr: 0.88 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
4.691IleAla: 4.691 ± 0.324
0.806IleCys: 0.806 ± 0.132
5.076IleAsp: 5.076 ± 0.353
5.204IleGlu: 5.204 ± 0.282
2.474IlePhe: 2.474 ± 0.235
3.702IleGly: 3.702 ± 0.266
1.136IleHis: 1.136 ± 0.161
4.05IleIle: 4.05 ± 0.272
5.278IleLys: 5.278 ± 0.361
3.977IleLeu: 3.977 ± 0.267
2.236IleMet: 2.236 ± 0.184
3.702IleAsn: 3.702 ± 0.258
2.584IlePro: 2.584 ± 0.217
2.62IleGln: 2.62 ± 0.239
3.152IleArg: 3.152 ± 0.227
3.977IleSer: 3.977 ± 0.282
4.196IleThr: 4.196 ± 0.24
4.398IleVal: 4.398 ± 0.304
0.385IleTrp: 0.385 ± 0.085
2.492IleTyr: 2.492 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
6.212LysAla: 6.212 ± 0.393
0.586LysCys: 0.586 ± 0.109
4.361LysAsp: 4.361 ± 0.302
5.901LysGlu: 5.901 ± 0.438
3.72LysPhe: 3.72 ± 0.303
4.27LysGly: 4.27 ± 0.303
1.539LysHis: 1.539 ± 0.172
4.691LysIle: 4.691 ± 0.307
4.783LysLys: 4.783 ± 0.365
5.791LysLeu: 5.791 ± 0.36
2.327LysMet: 2.327 ± 0.206
3.61LysAsn: 3.61 ± 0.278
2.492LysPro: 2.492 ± 0.258
2.657LysGln: 2.657 ± 0.25
3.225LysArg: 3.225 ± 0.255
3.775LysSer: 3.775 ± 0.278
3.628LysThr: 3.628 ± 0.217
5.241LysVal: 5.241 ± 0.324
0.935LysTrp: 0.935 ± 0.099
2.932LysTyr: 2.932 ± 0.253
0.0LysXaa: 0.0 ± 0.0
Leu
5.901LeuAla: 5.901 ± 0.34
0.733LeuCys: 0.733 ± 0.103
4.819LeuAsp: 4.819 ± 0.342
5.607LeuGlu: 5.607 ± 0.373
3.463LeuPhe: 3.463 ± 0.246
4.343LeuGly: 4.343 ± 0.27
1.393LeuHis: 1.393 ± 0.146
4.453LeuIle: 4.453 ± 0.245
6.487LeuLys: 6.487 ± 0.345
4.655LeuLeu: 4.655 ± 0.31
2.364LeuMet: 2.364 ± 0.191
4.398LeuAsn: 4.398 ± 0.27
3.463LeuPro: 3.463 ± 0.224
2.547LeuGln: 2.547 ± 0.21
3.628LeuArg: 3.628 ± 0.237
4.178LeuSer: 4.178 ± 0.277
4.856LeuThr: 4.856 ± 0.317
4.288LeuVal: 4.288 ± 0.275
0.935LeuTrp: 0.935 ± 0.149
2.767LeuTyr: 2.767 ± 0.241
0.0LeuXaa: 0.0 ± 0.0
Met
2.529MetAla: 2.529 ± 0.208
0.257MetCys: 0.257 ± 0.059
1.649MetAsp: 1.649 ± 0.177
1.979MetGlu: 1.979 ± 0.207
1.429MetPhe: 1.429 ± 0.161
1.723MetGly: 1.723 ± 0.181
0.531MetHis: 0.531 ± 0.099
1.961MetIle: 1.961 ± 0.197
2.694MetLys: 2.694 ± 0.25
2.144MetLeu: 2.144 ± 0.217
0.66MetMet: 0.66 ± 0.104
1.778MetAsn: 1.778 ± 0.179
0.898MetPro: 0.898 ± 0.128
1.191MetGln: 1.191 ± 0.156
1.448MetArg: 1.448 ± 0.156
1.906MetSer: 1.906 ± 0.199
1.942MetThr: 1.942 ± 0.18
1.851MetVal: 1.851 ± 0.198
0.312MetTrp: 0.312 ± 0.079
1.063MetTyr: 1.063 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.665AsnAla: 3.665 ± 0.311
0.623AsnCys: 0.623 ± 0.111
3.299AsnAsp: 3.299 ± 0.229
3.83AsnGlu: 3.83 ± 0.24
2.272AsnPhe: 2.272 ± 0.192
3.702AsnGly: 3.702 ± 0.296
0.861AsnHis: 0.861 ± 0.146
3.537AsnIle: 3.537 ± 0.21
3.518AsnLys: 3.518 ± 0.306
4.196AsnLeu: 4.196 ± 0.316
1.301AsnMet: 1.301 ± 0.165
2.73AsnAsn: 2.73 ± 0.269
2.474AsnPro: 2.474 ± 0.247
1.778AsnGln: 1.778 ± 0.195
2.364AsnArg: 2.364 ± 0.202
3.299AsnSer: 3.299 ± 0.266
2.511AsnThr: 2.511 ± 0.191
3.427AsnVal: 3.427 ± 0.351
0.696AsnTrp: 0.696 ± 0.113
1.961AsnTyr: 1.961 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
2.364ProAla: 2.364 ± 0.265
0.55ProCys: 0.55 ± 0.093
2.767ProAsp: 2.767 ± 0.232
2.987ProGlu: 2.987 ± 0.257
1.484ProPhe: 1.484 ± 0.15
2.602ProGly: 2.602 ± 0.25
0.715ProHis: 0.715 ± 0.108
1.906ProIle: 1.906 ± 0.192
2.62ProLys: 2.62 ± 0.219
2.492ProLeu: 2.492 ± 0.202
0.898ProMet: 0.898 ± 0.119
1.796ProAsn: 1.796 ± 0.227
1.154ProPro: 1.154 ± 0.155
1.081ProGln: 1.081 ± 0.131
1.411ProArg: 1.411 ± 0.155
2.675ProSer: 2.675 ± 0.228
2.126ProThr: 2.126 ± 0.224
3.024ProVal: 3.024 ± 0.275
0.88ProTrp: 0.88 ± 0.142
1.448ProTyr: 1.448 ± 0.172
0.0ProXaa: 0.0 ± 0.0
Gln
2.804GlnAla: 2.804 ± 0.268
0.348GlnCys: 0.348 ± 0.085
2.016GlnAsp: 2.016 ± 0.196
1.961GlnGlu: 1.961 ± 0.187
1.833GlnPhe: 1.833 ± 0.176
2.162GlnGly: 2.162 ± 0.173
0.678GlnHis: 0.678 ± 0.122
2.584GlnIle: 2.584 ± 0.222
2.382GlnLys: 2.382 ± 0.255
2.987GlnLeu: 2.987 ± 0.236
1.191GlnMet: 1.191 ± 0.133
1.448GlnAsn: 1.448 ± 0.164
1.338GlnPro: 1.338 ± 0.167
1.1GlnGln: 1.1 ± 0.156
1.997GlnArg: 1.997 ± 0.203
1.759GlnSer: 1.759 ± 0.184
2.254GlnThr: 2.254 ± 0.186
2.511GlnVal: 2.511 ± 0.196
0.898GlnTrp: 0.898 ± 0.135
1.649GlnTyr: 1.649 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
3.079ArgAla: 3.079 ± 0.246
0.641ArgCys: 0.641 ± 0.11
2.84ArgAsp: 2.84 ± 0.244
3.775ArgGlu: 3.775 ± 0.273
1.961ArgPhe: 1.961 ± 0.177
2.675ArgGly: 2.675 ± 0.248
0.88ArgHis: 0.88 ± 0.115
3.353ArgIle: 3.353 ± 0.253
3.134ArgLys: 3.134 ± 0.223
3.665ArgLeu: 3.665 ± 0.284
1.411ArgMet: 1.411 ± 0.169
2.437ArgAsn: 2.437 ± 0.216
1.484ArgPro: 1.484 ± 0.143
1.814ArgGln: 1.814 ± 0.184
1.961ArgArg: 1.961 ± 0.201
2.584ArgSer: 2.584 ± 0.21
2.236ArgThr: 2.236 ± 0.176
3.244ArgVal: 3.244 ± 0.251
0.77ArgTrp: 0.77 ± 0.131
1.741ArgTyr: 1.741 ± 0.157
0.0ArgXaa: 0.0 ± 0.0
Ser
3.867SerAla: 3.867 ± 0.29
0.586SerCys: 0.586 ± 0.113
3.757SerAsp: 3.757 ± 0.309
3.812SerGlu: 3.812 ± 0.228
2.657SerPhe: 2.657 ± 0.218
4.453SerGly: 4.453 ± 0.287
0.916SerHis: 0.916 ± 0.128
4.27SerIle: 4.27 ± 0.252
4.141SerLys: 4.141 ± 0.276
4.691SerLeu: 4.691 ± 0.294
1.558SerMet: 1.558 ± 0.181
2.932SerAsn: 2.932 ± 0.262
1.942SerPro: 1.942 ± 0.243
2.126SerGln: 2.126 ± 0.191
3.097SerArg: 3.097 ± 0.224
3.793SerSer: 3.793 ± 0.295
3.463SerThr: 3.463 ± 0.286
3.555SerVal: 3.555 ± 0.259
0.971SerTrp: 0.971 ± 0.132
2.584SerTyr: 2.584 ± 0.196
0.0SerXaa: 0.0 ± 0.0
Thr
3.903ThrAla: 3.903 ± 0.311
0.348ThrCys: 0.348 ± 0.095
3.5ThrAsp: 3.5 ± 0.301
3.793ThrGlu: 3.793 ± 0.279
2.382ThrPhe: 2.382 ± 0.224
4.27ThrGly: 4.27 ± 0.309
1.118ThrHis: 1.118 ± 0.145
4.105ThrIle: 4.105 ± 0.264
3.793ThrLys: 3.793 ± 0.293
4.196ThrLeu: 4.196 ± 0.267
1.503ThrMet: 1.503 ± 0.149
2.932ThrAsn: 2.932 ± 0.248
2.675ThrPro: 2.675 ± 0.256
2.016ThrGln: 2.016 ± 0.228
2.639ThrArg: 2.639 ± 0.212
3.353ThrSer: 3.353 ± 0.295
3.042ThrThr: 3.042 ± 0.271
3.94ThrVal: 3.94 ± 0.297
0.733ThrTrp: 0.733 ± 0.141
2.034ThrTyr: 2.034 ± 0.173
0.0ThrXaa: 0.0 ± 0.0
Val
4.398ValAla: 4.398 ± 0.312
0.953ValCys: 0.953 ± 0.131
4.563ValAsp: 4.563 ± 0.274
5.461ValGlu: 5.461 ± 0.306
2.932ValPhe: 2.932 ± 0.239
3.83ValGly: 3.83 ± 0.287
1.246ValHis: 1.246 ± 0.112
4.288ValIle: 4.288 ± 0.328
4.911ValLys: 4.911 ± 0.272
4.361ValLeu: 4.361 ± 0.316
1.942ValMet: 1.942 ± 0.177
3.683ValAsn: 3.683 ± 0.259
2.364ValPro: 2.364 ± 0.189
2.62ValGln: 2.62 ± 0.195
3.134ValArg: 3.134 ± 0.225
4.563ValSer: 4.563 ± 0.246
3.665ValThr: 3.665 ± 0.256
5.369ValVal: 5.369 ± 0.356
0.898ValTrp: 0.898 ± 0.123
3.005ValTyr: 3.005 ± 0.234
0.0ValXaa: 0.0 ± 0.0
Trp
1.081TrpAla: 1.081 ± 0.145
0.22TrpCys: 0.22 ± 0.071
0.99TrpAsp: 0.99 ± 0.145
0.88TrpGlu: 0.88 ± 0.14
0.733TrpPhe: 0.733 ± 0.118
0.641TrpGly: 0.641 ± 0.112
0.421TrpHis: 0.421 ± 0.085
0.678TrpIle: 0.678 ± 0.105
1.283TrpLys: 1.283 ± 0.157
1.008TrpLeu: 1.008 ± 0.132
0.733TrpMet: 0.733 ± 0.114
0.77TrpAsn: 0.77 ± 0.109
0.458TrpPro: 0.458 ± 0.1
0.568TrpGln: 0.568 ± 0.093
0.641TrpArg: 0.641 ± 0.113
0.623TrpSer: 0.623 ± 0.114
0.806TrpThr: 0.806 ± 0.149
1.045TrpVal: 1.045 ± 0.146
0.275TrpTrp: 0.275 ± 0.073
0.861TrpTyr: 0.861 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.877TyrAla: 2.877 ± 0.231
0.476TyrCys: 0.476 ± 0.089
2.804TyrAsp: 2.804 ± 0.215
2.419TyrGlu: 2.419 ± 0.191
1.924TyrPhe: 1.924 ± 0.18
2.346TyrGly: 2.346 ± 0.217
0.751TyrHis: 0.751 ± 0.124
2.639TyrIle: 2.639 ± 0.229
3.042TyrLys: 3.042 ± 0.227
3.042TyrLeu: 3.042 ± 0.24
1.173TyrMet: 1.173 ± 0.174
2.291TyrAsn: 2.291 ± 0.22
1.796TyrPro: 1.796 ± 0.145
1.631TyrGln: 1.631 ± 0.168
1.869TyrArg: 1.869 ± 0.184
2.382TyrSer: 2.382 ± 0.199
2.272TyrThr: 2.272 ± 0.252
2.804TyrVal: 2.804 ± 0.227
0.605TyrTrp: 0.605 ± 0.103
1.668TyrTyr: 1.668 ± 0.189
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 296 proteins (54571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski