Bifidobacterium callitrichos DSM 23973
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2362 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A087A2Z1|A0A087A2Z1_9BIFI Transport protein OS=Bifidobacterium callitrichos DSM 23973 OX=1437609 GN=BCAL_2042 PE=4 SV=1
MM1 pKa = 7.13 AQDD4 pKa = 3.51 YY5 pKa = 10.08 DD6 pKa = 3.82 SPRR9 pKa = 11.84 NKK11 pKa = 10.84 DD12 pKa = 3.11 EE13 pKa = 5.7 DD14 pKa = 4.16 EE15 pKa = 4.93 EE16 pKa = 4.45 SLQALGKK23 pKa = 10.1 SSQNSSSDD31 pKa = 3.01 IDD33 pKa = 3.98 DD34 pKa = 4.76 DD35 pKa = 4.39 EE36 pKa = 4.37 NAIAEE41 pKa = 4.35 DD42 pKa = 4.23 YY43 pKa = 9.11 EE44 pKa = 5.05 LPGADD49 pKa = 4.92 LSNEE53 pKa = 4.01 DD54 pKa = 3.61 SSVTVIPMQGDD65 pKa = 3.48 EE66 pKa = 5.44 FICSEE71 pKa = 4.23 CFLVKK76 pKa = 10.28 HH77 pKa = 6.45 RR78 pKa = 11.84 SQLAYY83 pKa = 8.35 MTDD86 pKa = 3.79 DD87 pKa = 4.14 GQPVCQEE94 pKa = 4.08 CAAA97 pKa = 4.29
Molecular weight: 10.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.617
IPC_protein 3.592
Toseland 3.389
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.541
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.719
Patrickios 0.846
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.678
Protein with the highest isoelectric point:
>tr|A0A087A0P9|A0A087A0P9_9BIFI Lysophospholipase OS=Bifidobacterium callitrichos DSM 23973 OX=1437609 GN=BCAL_1994 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.84 MKK15 pKa = 9.68 HH16 pKa = 5.75 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 6.41 TLSAA44 pKa = 4.15
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2362
0
2362
826231
21
1604
349.8
38.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.145 ± 0.059
0.876 ± 0.017
6.805 ± 0.044
5.529 ± 0.054
3.417 ± 0.03
8.252 ± 0.042
2.151 ± 0.026
5.355 ± 0.037
3.642 ± 0.048
8.798 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.605 ± 0.026
3.164 ± 0.032
4.752 ± 0.034
3.038 ± 0.028
6.432 ± 0.061
5.967 ± 0.043
6.187 ± 0.045
7.733 ± 0.041
1.419 ± 0.019
2.732 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here