Ixeridium yellow mottle virus 1
Average proteome isoelectric point is 7.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142D6T2|A0A142D6T2_9LUTE Serine protease OS=Ixeridium yellow mottle virus 1 OX=1809767 PE=4 SV=1
MM1 pKa = 7.56 EE2 pKa = 4.32 KK3 pKa = 10.53 HH4 pKa = 6.21 SIKK7 pKa = 10.74 LFLLGLFFSSFPFFFKK23 pKa = 10.85 NAFASSEE30 pKa = 4.2 MEE32 pKa = 4.13 LSSSLALNQSPFSNGLYY49 pKa = 10.21 SPVISRR55 pKa = 11.84 SLQCPISYY63 pKa = 10.5 EE64 pKa = 4.23 LICVEE69 pKa = 4.7 KK70 pKa = 10.59 PPGEE74 pKa = 4.73 LISEE78 pKa = 4.53 SYY80 pKa = 11.16 NDD82 pKa = 5.15 LIQALLEE89 pKa = 4.02 KK90 pKa = 10.38 SYY92 pKa = 11.89 ADD94 pKa = 3.12 ALSYY98 pKa = 10.03 YY99 pKa = 9.86 FRR101 pKa = 11.84 ALEE104 pKa = 3.91 TCARR108 pKa = 11.84 GFATFIGYY116 pKa = 9.32 SGRR119 pKa = 11.84 ILSDD123 pKa = 3.78 LVSMTLWFAIFLWLHH138 pKa = 5.05 TFGVILSIVYY148 pKa = 10.64 YY149 pKa = 11.08 LMTQHH154 pKa = 6.55 TVPTLILVSLTLLTRR169 pKa = 11.84 YY170 pKa = 9.97 IIRR173 pKa = 11.84 RR174 pKa = 11.84 TEE176 pKa = 3.8 WVFGEE181 pKa = 4.09 LPYY184 pKa = 10.92 FVMRR188 pKa = 11.84 ASCFLLRR195 pKa = 11.84 SSYY198 pKa = 10.49 QALSSSKK205 pKa = 10.61 SYY207 pKa = 10.43 IRR209 pKa = 11.84 EE210 pKa = 3.71 KK211 pKa = 10.8 AVAGFTSYY219 pKa = 10.84 SIPQAPPKK227 pKa = 10.67 NSVLEE232 pKa = 4.09 LVHH235 pKa = 7.14 PDD237 pKa = 3.5 GSHH240 pKa = 7.08 LGYY243 pKa = 9.08 ATCISLFNGEE253 pKa = 4.26 NALITALHH261 pKa = 6.09 CVNGNALVKK270 pKa = 10.65 GKK272 pKa = 8.43 TGNKK276 pKa = 9.29 IPLKK280 pKa = 9.78 EE281 pKa = 3.65 FAARR285 pKa = 11.84 IEE287 pKa = 4.45 DD288 pKa = 3.72 KK289 pKa = 11.28 GSDD292 pKa = 3.42 FALFSGPPSWEE303 pKa = 3.87 SLVGCKK309 pKa = 9.11 GARR312 pKa = 11.84 FVTSNDD318 pKa = 3.15 LARR321 pKa = 11.84 CSASLYY327 pKa = 10.93 KK328 pKa = 9.96 LTEE331 pKa = 4.43 SGWISTNAKK340 pKa = 9.6 VVGPYY345 pKa = 9.47 EE346 pKa = 3.89 NRR348 pKa = 11.84 VSVLSEE354 pKa = 3.98 TFPGDD359 pKa = 2.9 SGAPYY364 pKa = 9.99 WNGKK368 pKa = 5.94 TVLGVHH374 pKa = 6.51 TGAPTHH380 pKa = 6.04 EE381 pKa = 5.37 NINVMTPIPHH391 pKa = 6.54 IPGVTAPIYY400 pKa = 10.4 VYY402 pKa = 8.85 EE403 pKa = 4.34 TTAPQGKK410 pKa = 9.17 IFAKK414 pKa = 10.07 PLNPPKK420 pKa = 10.49 YY421 pKa = 10.32 KK422 pKa = 10.17 PIKK425 pKa = 9.41 LQNHH429 pKa = 5.4 WDD431 pKa = 3.65 DD432 pKa = 4.46 RR433 pKa = 11.84 EE434 pKa = 4.38 SEE436 pKa = 4.28 GSSFYY441 pKa = 10.81 DD442 pKa = 3.14 DD443 pKa = 4.41 PEE445 pKa = 3.97 FLRR448 pKa = 11.84 SFKK451 pKa = 9.51 STKK454 pKa = 9.54 SMKK457 pKa = 10.48 AKK459 pKa = 10.32 FIEE462 pKa = 4.42 EE463 pKa = 4.45 CEE465 pKa = 4.32 EE466 pKa = 4.2 GDD468 pKa = 3.94 HH469 pKa = 7.48 VISAWLYY476 pKa = 9.79 SEE478 pKa = 4.39 TKK480 pKa = 10.39 EE481 pKa = 4.48 STFLQWATTLLDD493 pKa = 4.9 AILNKK498 pKa = 10.45 APFEE502 pKa = 4.34 HH503 pKa = 7.38 DD504 pKa = 3.49 VYY506 pKa = 11.61 GDD508 pKa = 2.95 ISTWFNTSEE517 pKa = 4.02 VLTAIRR523 pKa = 11.84 SYY525 pKa = 10.48 EE526 pKa = 4.12 ANLKK530 pKa = 8.76 ATDD533 pKa = 3.47 IYY535 pKa = 10.49 EE536 pKa = 4.22 EE537 pKa = 4.39 SVSVHH542 pKa = 5.64 TSDD545 pKa = 3.46 TDD547 pKa = 3.92 SVATAPSFVKK557 pKa = 10.59 EE558 pKa = 4.09 SLNRR562 pKa = 11.84 ARR564 pKa = 11.84 QHH566 pKa = 6.48 RR567 pKa = 11.84 LPTNRR572 pKa = 11.84 KK573 pKa = 7.76 PRR575 pKa = 11.84 SKK577 pKa = 10.38 HH578 pKa = 4.74 RR579 pKa = 11.84 RR580 pKa = 11.84 HH581 pKa = 6.3 PKK583 pKa = 9.95 NYY585 pKa = 8.4 AKK587 pKa = 10.2 EE588 pKa = 3.7 WRR590 pKa = 11.84 RR591 pKa = 11.84 NNGTDD596 pKa = 3.05 PVTIISEE603 pKa = 4.19 DD604 pKa = 3.27 QFQGNRR610 pKa = 11.84 GQDD613 pKa = 3.33 CQRR616 pKa = 11.84 GVKK619 pKa = 10.28 EE620 pKa = 4.15 SDD622 pKa = 2.84 ADD624 pKa = 3.93 RR625 pKa = 11.84 AKK627 pKa = 10.05 TRR629 pKa = 11.84 RR630 pKa = 11.84 EE631 pKa = 3.58 RR632 pKa = 11.84 TEE634 pKa = 4.23 NFSQYY639 pKa = 10.5 FKK641 pKa = 11.21 EE642 pKa = 4.06 KK643 pKa = 10.82 YY644 pKa = 8.67 NWEE647 pKa = 4.09 TEE649 pKa = 3.99 GRR651 pKa = 11.84 FSDD654 pKa = 3.2 IPGFRR659 pKa = 11.84 DD660 pKa = 3.47 CGSIPSYY667 pKa = 9.4 YY668 pKa = 9.71 HH669 pKa = 6.92 PKK671 pKa = 10.32 GKK673 pKa = 10.08 QNSGWGEE680 pKa = 3.85 EE681 pKa = 4.18 MVANYY686 pKa = 9.61 PEE688 pKa = 4.76 LGEE691 pKa = 4.05 AVKK694 pKa = 10.94 GFGWPQFGAHH704 pKa = 6.93 AEE706 pKa = 4.21 LKK708 pKa = 10.44 SLRR711 pKa = 11.84 LQSARR716 pKa = 11.84 WLEE719 pKa = 3.92 RR720 pKa = 11.84 LKK722 pKa = 10.81 SAKK725 pKa = 9.74 IPSSEE730 pKa = 3.75 EE731 pKa = 3.6 RR732 pKa = 11.84 EE733 pKa = 4.12 AVIMKK738 pKa = 8.05 TLKK741 pKa = 10.26 AYY743 pKa = 9.88 KK744 pKa = 9.56 PVEE747 pKa = 4.12 TQAPDD752 pKa = 2.91 IFNAKK757 pKa = 9.37 EE758 pKa = 4.21 DD759 pKa = 3.69 ALDD762 pKa = 3.21 WKK764 pKa = 10.91 KK765 pKa = 10.4 FLEE768 pKa = 4.3 VFKK771 pKa = 11.18 VAVHH775 pKa = 5.72 SLEE778 pKa = 4.05 LDD780 pKa = 3.19 AGIGVPYY787 pKa = 9.92 IAYY790 pKa = 8.45 GKK792 pKa = 6.46 PTHH795 pKa = 6.62 RR796 pKa = 11.84 GWVEE800 pKa = 3.94 DD801 pKa = 4.19 PEE803 pKa = 4.58 LLPVLAQLTFDD814 pKa = 4.59 RR815 pKa = 11.84 LQKK818 pKa = 9.43 MLEE821 pKa = 4.11 VGYY824 pKa = 9.34 EE825 pKa = 3.82 AHH827 pKa = 7.27 LSPEE831 pKa = 3.85 QRR833 pKa = 11.84 VQMGLCDD840 pKa = 5.04 PIRR843 pKa = 11.84 VFVKK847 pKa = 10.78 GEE849 pKa = 3.59 PHH851 pKa = 6.19 KK852 pKa = 10.54 QSKK855 pKa = 10.21 LDD857 pKa = 3.38 EE858 pKa = 4.27 GRR860 pKa = 11.84 YY861 pKa = 8.96 RR862 pKa = 11.84 LIMSVSLVDD871 pKa = 3.45 QLVARR876 pKa = 11.84 VLFQHH881 pKa = 5.11 QNKK884 pKa = 9.72 RR885 pKa = 11.84 EE886 pKa = 3.77 IDD888 pKa = 3.24 LWKK891 pKa = 10.49 AIPSKK896 pKa = 10.62 PGFGLSTDD904 pKa = 3.65 DD905 pKa = 3.46 QTVEE909 pKa = 4.4 FVKK912 pKa = 10.96 LLAKK916 pKa = 9.84 QANLQVDD923 pKa = 4.28 EE924 pKa = 5.62 LLQDD928 pKa = 3.88 WEE930 pKa = 4.21 KK931 pKa = 11.05 HH932 pKa = 5.81 LYY934 pKa = 7.83 PTDD937 pKa = 3.45 CSGFDD942 pKa = 3.27 WSVSQWMLIDD952 pKa = 4.57 DD953 pKa = 4.33 LLVRR957 pKa = 11.84 QSLQTSSFKK966 pKa = 10.66 FDD968 pKa = 2.61 ILQRR972 pKa = 11.84 VWTDD976 pKa = 3.21 CLGNSVLALSDD987 pKa = 3.49 GRR989 pKa = 11.84 LLAQTIPGIQKK1000 pKa = 9.54 SGSYY1004 pKa = 8.29 NTSSTNSRR1012 pKa = 11.84 IRR1014 pKa = 11.84 VMAAFHH1020 pKa = 6.85 CGASWAIAMGDD1031 pKa = 3.62 DD1032 pKa = 3.6 ALEE1035 pKa = 4.63 SKK1037 pKa = 10.96 DD1038 pKa = 3.8 SDD1040 pKa = 3.43 LSRR1043 pKa = 11.84 YY1044 pKa = 10.0 AEE1046 pKa = 4.14 LGFKK1050 pKa = 10.74 VEE1052 pKa = 4.22 VSAKK1056 pKa = 10.8 LEE1058 pKa = 4.14 FCSHH1062 pKa = 6.65 IFEE1065 pKa = 4.48 QLNLAIPVNKK1075 pKa = 10.27 GKK1077 pKa = 8.79 MLYY1080 pKa = 10.38 KK1081 pKa = 10.66 LIFGYY1086 pKa = 10.74 DD1087 pKa = 3.62 PGCGNQEE1094 pKa = 3.95 VVKK1097 pKa = 10.62 NYY1099 pKa = 10.58 LDD1101 pKa = 3.66 ALFSVLHH1108 pKa = 5.97 EE1109 pKa = 4.48 LRR1111 pKa = 11.84 HH1112 pKa = 6.22 DD1113 pKa = 3.91 PEE1115 pKa = 4.48 LVQRR1119 pKa = 11.84 LHH1121 pKa = 5.86 GWLVPVV1127 pKa = 4.43
Molecular weight: 127.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.175
IPC2_protein 6.186
IPC_protein 6.262
Toseland 6.547
ProMoST 6.664
Dawson 6.59
Bjellqvist 6.59
Wikipedia 6.605
Rodwell 6.59
Grimsley 6.678
Solomon 6.59
Lehninger 6.59
Nozaki 6.883
DTASelect 7.044
Thurlkill 7.073
EMBOSS 7.044
Sillero 6.985
Patrickios 4.202
IPC_peptide 6.62
IPC2_peptide 6.795
IPC2.peptide.svr19 6.792
Protein with the highest isoelectric point:
>tr|A0A142D6T5|A0A142D6T5_9LUTE Movement protein OS=Ixeridium yellow mottle virus 1 OX=1809767 PE=4 SV=1
MM1 pKa = 6.79 NTGAGRR7 pKa = 11.84 NVGRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 TNTIPRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 NRR26 pKa = 11.84 VVVVQASRR34 pKa = 11.84 QPQRR38 pKa = 11.84 GARR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 VRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 PAGGSGVGVRR58 pKa = 11.84 RR59 pKa = 11.84 SRR61 pKa = 11.84 EE62 pKa = 3.88 TFVFTKK68 pKa = 10.73 DD69 pKa = 3.5 SIAGSASGSITFGPSLSEE87 pKa = 4.04 SPAFSSGILRR97 pKa = 11.84 AYY99 pKa = 10.19 HH100 pKa = 6.41 EE101 pKa = 4.51 YY102 pKa = 10.47 KK103 pKa = 9.26 ITMVKK108 pKa = 10.64 LEE110 pKa = 4.9 FISEE114 pKa = 4.18 ASSTSSGSISYY125 pKa = 10.24 EE126 pKa = 3.71 LDD128 pKa = 3.27 PHH130 pKa = 6.74 CKK132 pKa = 9.93 SSSLQSTVNKK142 pKa = 10.31 FGITKK147 pKa = 10.34 NGAKK151 pKa = 8.62 TWTARR156 pKa = 11.84 LINGQEE162 pKa = 3.63 WHH164 pKa = 7.45 DD165 pKa = 3.62 ATEE168 pKa = 4.03 DD169 pKa = 3.41 QFRR172 pKa = 11.84 ILYY175 pKa = 8.98 KK176 pKa = 10.99 GNGASSVAGSFRR188 pKa = 11.84 ITITCQLQNPKK199 pKa = 10.55
Molecular weight: 22.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.162
IPC2_protein 10.175
IPC_protein 11.52
Toseland 11.667
ProMoST 12.135
Dawson 11.681
Bjellqvist 11.652
Wikipedia 12.135
Rodwell 11.462
Grimsley 11.711
Solomon 12.149
Lehninger 12.047
Nozaki 11.667
DTASelect 11.652
Thurlkill 11.667
EMBOSS 12.149
Sillero 11.667
Patrickios 11.169
IPC_peptide 12.149
IPC2_peptide 11.125
IPC2.peptide.svr19 9.38
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3125
183
1127
520.8
58.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.688 ± 0.248
1.408 ± 0.121
4.288 ± 0.434
6.528 ± 0.28
4.448 ± 0.5
6.528 ± 0.37
2.176 ± 0.215
4.928 ± 0.341
5.44 ± 0.622
8.64 ± 1.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.472 ± 0.112
4.16 ± 0.358
6.24 ± 0.674
3.552 ± 0.51
6.464 ± 1.098
10.112 ± 0.699
6.048 ± 0.608
5.568 ± 0.245
1.888 ± 0.159
3.392 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here