Duck faeces associated circular DNA virus 2
Average proteome isoelectric point is 7.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160HX33|A0A160HX33_9VIRU Replication associated protein OS=Duck faeces associated circular DNA virus 2 OX=1843769 PE=3 SV=1
MM1 pKa = 6.56 TTPIAEE7 pKa = 4.51 DD8 pKa = 3.42 NDD10 pKa = 3.83 FKK12 pKa = 11.37 FQGIRR17 pKa = 11.84 ALLTYY22 pKa = 7.05 PQCNEE27 pKa = 3.49 GSIEE31 pKa = 3.94 EE32 pKa = 4.57 LLTFFKK38 pKa = 10.62 TIFGNKK44 pKa = 7.91 IEE46 pKa = 4.56 YY47 pKa = 10.07 IVVCKK52 pKa = 10.21 EE53 pKa = 3.69 NHH55 pKa = 6.35 HH56 pKa = 5.74 EE57 pKa = 4.13 TDD59 pKa = 3.44 GEE61 pKa = 4.45 HH62 pKa = 4.54 YY63 pKa = 10.26 HH64 pKa = 7.94 AYY66 pKa = 9.82 IKK68 pKa = 10.77 FNTRR72 pKa = 11.84 TRR74 pKa = 11.84 VYY76 pKa = 11.07 ANNLVYY82 pKa = 10.48 RR83 pKa = 11.84 GVRR86 pKa = 11.84 PNIEE90 pKa = 4.02 KK91 pKa = 8.83 VTRR94 pKa = 11.84 TAYY97 pKa = 10.5 AAVNYY102 pKa = 9.3 VKK104 pKa = 10.66 KK105 pKa = 10.68 DD106 pKa = 3.51 GNFKK110 pKa = 11.11 EE111 pKa = 5.13 EE112 pKa = 4.52 GTCTDD117 pKa = 2.95 IKK119 pKa = 11.08 KK120 pKa = 8.77 LTTQEE125 pKa = 3.78 KK126 pKa = 10.0 YY127 pKa = 10.97 KK128 pKa = 10.67 LIKK131 pKa = 8.46 EE132 pKa = 4.12 KK133 pKa = 10.59 KK134 pKa = 7.93 YY135 pKa = 10.73 IEE137 pKa = 4.24 IFEE140 pKa = 4.41 MATLSLPEE148 pKa = 4.11 LCKK151 pKa = 10.6 VKK153 pKa = 10.45 QIQRR157 pKa = 11.84 EE158 pKa = 4.21 LIINDD163 pKa = 3.41 WPLDD167 pKa = 3.45 GSKK170 pKa = 10.34 NRR172 pKa = 11.84 KK173 pKa = 9.28 VYY175 pKa = 9.31 WFYY178 pKa = 11.73 GKK180 pKa = 8.6 TGTGKK185 pKa = 8.1 TRR187 pKa = 11.84 EE188 pKa = 4.23 ATKK191 pKa = 10.61 MMIEE195 pKa = 4.62 LYY197 pKa = 10.25 GEE199 pKa = 3.88 NWISLTGDD207 pKa = 3.03 LRR209 pKa = 11.84 TFFDD213 pKa = 4.61 PYY215 pKa = 10.64 NGEE218 pKa = 3.97 KK219 pKa = 10.68 GVIFDD224 pKa = 4.59 DD225 pKa = 3.72 VRR227 pKa = 11.84 KK228 pKa = 10.38 GSIIWNTLLTITDD241 pKa = 4.2 RR242 pKa = 11.84 YY243 pKa = 7.67 RR244 pKa = 11.84 TSVNVKK250 pKa = 9.63 GSRR253 pKa = 11.84 IPWLAEE259 pKa = 3.81 TIIFTSPQHH268 pKa = 4.68 FTEE271 pKa = 4.38 VFTTEE276 pKa = 3.84 KK277 pKa = 10.66 DD278 pKa = 3.35 GEE280 pKa = 4.16 RR281 pKa = 11.84 AQWDD285 pKa = 3.68 GLEE288 pKa = 3.96 QFEE291 pKa = 5.08 RR292 pKa = 11.84 RR293 pKa = 11.84 ISEE296 pKa = 3.63 IKK298 pKa = 10.33 EE299 pKa = 3.88 FNN301 pKa = 3.27
Molecular weight: 35.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.922
IPC2_protein 7.322
IPC_protein 7.351
Toseland 7.146
ProMoST 7.775
Dawson 8.039
Bjellqvist 8.156
Wikipedia 7.98
Rodwell 8.039
Grimsley 7.176
Solomon 8.17
Lehninger 8.17
Nozaki 8.302
DTASelect 8.068
Thurlkill 8.141
EMBOSS 8.258
Sillero 8.39
Patrickios 4.406
IPC_peptide 8.17
IPC2_peptide 7.161
IPC2.peptide.svr19 7.53
Protein with the highest isoelectric point:
>tr|A0A160HX33|A0A160HX33_9VIRU Replication associated protein OS=Duck faeces associated circular DNA virus 2 OX=1843769 PE=3 SV=1
MM1 pKa = 7.28 PRR3 pKa = 11.84 YY4 pKa = 9.47 YY5 pKa = 10.39 KK6 pKa = 10.29 YY7 pKa = 10.12 KK8 pKa = 8.93 RR9 pKa = 11.84 YY10 pKa = 8.87 YY11 pKa = 10.19 RR12 pKa = 11.84 RR13 pKa = 11.84 MYY15 pKa = 9.38 PKK17 pKa = 10.21 KK18 pKa = 9.42 RR19 pKa = 11.84 WASNIFTEE27 pKa = 4.22 NNIVTVLASQKK38 pKa = 10.7 DD39 pKa = 3.79 AFASTTICANSPQTDD54 pKa = 3.48 VPTPVIVKK62 pKa = 9.31 FGRR65 pKa = 11.84 CKK67 pKa = 10.4 VKK69 pKa = 10.74 GDD71 pKa = 3.45 VRR73 pKa = 11.84 TDD75 pKa = 3.33 LDD77 pKa = 3.5 NGNNFVSATMYY88 pKa = 10.31 LAYY91 pKa = 10.52 VPEE94 pKa = 5.45 GYY96 pKa = 9.73 PIADD100 pKa = 3.91 LPKK103 pKa = 10.11 LHH105 pKa = 7.36 PEE107 pKa = 4.23 YY108 pKa = 10.47 IIGWTQISLDD118 pKa = 3.74 SGNTFSFSSPLKK130 pKa = 10.41 RR131 pKa = 11.84 NLNSGDD137 pKa = 3.7 KK138 pKa = 10.69 IMMLFYY144 pKa = 11.01 VSATNTAQYY153 pKa = 10.56 VRR155 pKa = 11.84 AFNFYY160 pKa = 9.53 YY161 pKa = 9.8 TVQYY165 pKa = 7.15 WTTSAA170 pKa = 3.94
Molecular weight: 19.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.766
IPC2_protein 9.209
IPC_protein 9.165
Toseland 9.355
ProMoST 9.311
Dawson 9.721
Bjellqvist 9.516
Wikipedia 9.97
Rodwell 9.853
Grimsley 9.853
Solomon 9.765
Lehninger 9.706
Nozaki 9.428
DTASelect 9.502
Thurlkill 9.531
EMBOSS 9.809
Sillero 9.663
Patrickios 3.897
IPC_peptide 9.75
IPC2_peptide 8.302
IPC2.peptide.svr19 7.974
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
471
170
301
235.5
27.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.883 ± 1.203
1.274 ± 0.054
4.671 ± 0.019
6.794 ± 2.78
5.52 ± 0.125
5.52 ± 0.775
1.274 ± 0.379
7.431 ± 1.181
8.28 ± 1.0
5.945 ± 0.36
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.911 ± 0.57
6.157 ± 0.499
3.822 ± 1.139
2.76 ± 0.1
5.52 ± 0.125
4.671 ± 1.645
9.554 ± 0.404
5.732 ± 0.733
1.911 ± 0.081
6.369 ± 1.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here