Amino acid dipepetide frequency for Pseudomonas phage ZC03

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.967AlaAla: 5.967 ± 0.765
0.551AlaCys: 0.551 ± 0.204
3.534AlaAsp: 3.534 ± 0.425
5.003AlaGlu: 5.003 ± 0.542
2.891AlaPhe: 2.891 ± 0.345
4.039AlaGly: 4.039 ± 0.565
1.056AlaHis: 1.056 ± 0.169
5.278AlaIle: 5.278 ± 0.403
4.223AlaLys: 4.223 ± 0.452
6.93AlaLeu: 6.93 ± 0.848
1.79AlaMet: 1.79 ± 0.298
3.259AlaAsn: 3.259 ± 0.526
2.341AlaPro: 2.341 ± 0.302
3.075AlaGln: 3.075 ± 0.355
3.534AlaArg: 3.534 ± 0.416
4.223AlaSer: 4.223 ± 0.498
3.672AlaThr: 3.672 ± 0.377
5.003AlaVal: 5.003 ± 0.67
1.193AlaTrp: 1.193 ± 0.276
2.433AlaTyr: 2.433 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.505CysAla: 0.505 ± 0.173
0.046CysCys: 0.046 ± 0.046
0.551CysAsp: 0.551 ± 0.18
0.505CysGlu: 0.505 ± 0.179
0.459CysPhe: 0.459 ± 0.184
0.275CysGly: 0.275 ± 0.127
0.138CysHis: 0.138 ± 0.099
0.275CysIle: 0.275 ± 0.115
0.78CysLys: 0.78 ± 0.24
1.01CysLeu: 1.01 ± 0.256
0.229CysMet: 0.229 ± 0.11
0.459CysAsn: 0.459 ± 0.192
0.505CysPro: 0.505 ± 0.197
0.138CysGln: 0.138 ± 0.083
0.688CysArg: 0.688 ± 0.257
0.78CysSer: 0.78 ± 0.236
0.367CysThr: 0.367 ± 0.152
0.229CysVal: 0.229 ± 0.118
0.275CysTrp: 0.275 ± 0.132
0.275CysTyr: 0.275 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
4.911AspAla: 4.911 ± 0.452
0.551AspCys: 0.551 ± 0.215
2.891AspAsp: 2.891 ± 0.396
4.406AspGlu: 4.406 ± 0.386
2.387AspPhe: 2.387 ± 0.348
3.672AspGly: 3.672 ± 0.517
1.193AspHis: 1.193 ± 0.288
4.544AspIle: 4.544 ± 0.47
3.121AspLys: 3.121 ± 0.392
5.737AspLeu: 5.737 ± 0.533
1.377AspMet: 1.377 ± 0.261
2.983AspAsn: 2.983 ± 0.354
3.442AspPro: 3.442 ± 0.368
2.249AspGln: 2.249 ± 0.31
2.111AspArg: 2.111 ± 0.262
3.901AspSer: 3.901 ± 0.44
2.616AspThr: 2.616 ± 0.325
4.039AspVal: 4.039 ± 0.341
0.872AspTrp: 0.872 ± 0.196
2.157AspTyr: 2.157 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
6.563GluAla: 6.563 ± 0.702
0.826GluCys: 0.826 ± 0.247
4.452GluAsp: 4.452 ± 0.594
7.16GluGlu: 7.16 ± 1.281
3.626GluPhe: 3.626 ± 0.478
3.764GluGly: 3.764 ± 0.409
1.698GluHis: 1.698 ± 0.284
5.14GluIle: 5.14 ± 0.494
4.773GluLys: 4.773 ± 0.636
6.242GluLeu: 6.242 ± 0.727
1.79GluMet: 1.79 ± 0.312
4.223GluAsn: 4.223 ± 0.437
3.396GluPro: 3.396 ± 0.534
2.662GluGln: 2.662 ± 0.551
2.478GluArg: 2.478 ± 0.26
4.773GluSer: 4.773 ± 0.813
4.957GluThr: 4.957 ± 0.63
5.232GluVal: 5.232 ± 0.421
1.056GluTrp: 1.056 ± 0.208
2.846GluTyr: 2.846 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
2.019PheAla: 2.019 ± 0.263
0.367PheCys: 0.367 ± 0.157
2.937PheAsp: 2.937 ± 0.341
2.203PheGlu: 2.203 ± 0.334
1.331PhePhe: 1.331 ± 0.269
2.295PheGly: 2.295 ± 0.286
0.78PheHis: 0.78 ± 0.232
2.524PheIle: 2.524 ± 0.29
2.983PheLys: 2.983 ± 0.325
3.442PheLeu: 3.442 ± 0.477
1.102PheMet: 1.102 ± 0.226
2.524PheAsn: 2.524 ± 0.275
1.469PhePro: 1.469 ± 0.274
1.423PheGln: 1.423 ± 0.203
2.57PheArg: 2.57 ± 0.257
2.387PheSer: 2.387 ± 0.359
2.478PheThr: 2.478 ± 0.366
2.065PheVal: 2.065 ± 0.396
0.321PheTrp: 0.321 ± 0.125
1.698PheTyr: 1.698 ± 0.34
0.092PheXaa: 0.092 ± 0.1
Gly
3.305GlyAla: 3.305 ± 0.484
0.551GlyCys: 0.551 ± 0.207
2.846GlyAsp: 2.846 ± 0.496
3.764GlyGlu: 3.764 ± 0.426
3.029GlyPhe: 3.029 ± 0.34
3.855GlyGly: 3.855 ± 0.609
0.643GlyHis: 0.643 ± 0.173
4.131GlyIle: 4.131 ± 0.497
4.406GlyLys: 4.406 ± 0.36
4.59GlyLeu: 4.59 ± 0.47
1.974GlyMet: 1.974 ± 0.325
3.855GlyAsn: 3.855 ± 0.383
1.331GlyPro: 1.331 ± 0.262
1.882GlyGln: 1.882 ± 0.305
3.167GlyArg: 3.167 ± 0.326
5.324GlySer: 5.324 ± 0.542
3.534GlyThr: 3.534 ± 0.259
3.993GlyVal: 3.993 ± 0.451
1.056GlyTrp: 1.056 ± 0.347
2.708GlyTyr: 2.708 ± 0.38
0.046GlyXaa: 0.046 ± 0.045
His
1.469HisAla: 1.469 ± 0.264
0.184HisCys: 0.184 ± 0.101
0.872HisAsp: 0.872 ± 0.2
1.744HisGlu: 1.744 ± 0.28
0.918HisPhe: 0.918 ± 0.242
0.826HisGly: 0.826 ± 0.172
0.413HisHis: 0.413 ± 0.151
1.331HisIle: 1.331 ± 0.235
0.643HisLys: 0.643 ± 0.17
1.928HisLeu: 1.928 ± 0.355
0.413HisMet: 0.413 ± 0.12
0.918HisAsn: 0.918 ± 0.207
1.147HisPro: 1.147 ± 0.233
0.505HisGln: 0.505 ± 0.161
0.964HisArg: 0.964 ± 0.212
0.918HisSer: 0.918 ± 0.201
0.551HisThr: 0.551 ± 0.117
1.147HisVal: 1.147 ± 0.233
0.321HisTrp: 0.321 ± 0.114
0.78HisTyr: 0.78 ± 0.21
0.046HisXaa: 0.046 ± 0.045
Ile
4.452IleAla: 4.452 ± 0.487
0.734IleCys: 0.734 ± 0.239
4.085IleAsp: 4.085 ± 0.451
5.186IleGlu: 5.186 ± 0.499
1.79IlePhe: 1.79 ± 0.256
3.672IleGly: 3.672 ± 0.599
1.515IleHis: 1.515 ± 0.258
2.754IleIle: 2.754 ± 0.3
4.819IleLys: 4.819 ± 0.624
6.196IleLeu: 6.196 ± 0.598
0.734IleMet: 0.734 ± 0.181
4.452IleAsn: 4.452 ± 0.462
3.442IlePro: 3.442 ± 0.44
2.754IleGln: 2.754 ± 0.307
3.809IleArg: 3.809 ± 0.429
4.636IleSer: 4.636 ± 0.462
3.993IleThr: 3.993 ± 0.547
3.029IleVal: 3.029 ± 0.42
0.643IleTrp: 0.643 ± 0.16
2.019IleTyr: 2.019 ± 0.271
0.0IleXaa: 0.0 ± 0.0
Lys
4.957LysAla: 4.957 ± 0.512
0.367LysCys: 0.367 ± 0.125
4.177LysAsp: 4.177 ± 0.551
5.278LysGlu: 5.278 ± 0.732
2.295LysPhe: 2.295 ± 0.282
3.442LysGly: 3.442 ± 0.364
1.331LysHis: 1.331 ± 0.252
3.121LysIle: 3.121 ± 0.386
4.085LysLys: 4.085 ± 0.452
6.15LysLeu: 6.15 ± 0.598
1.469LysMet: 1.469 ± 0.294
4.177LysAsn: 4.177 ± 0.412
2.616LysPro: 2.616 ± 0.307
2.57LysGln: 2.57 ± 0.296
2.891LysArg: 2.891 ± 0.382
3.58LysSer: 3.58 ± 0.429
3.947LysThr: 3.947 ± 0.358
3.35LysVal: 3.35 ± 0.488
0.688LysTrp: 0.688 ± 0.196
2.891LysTyr: 2.891 ± 0.416
0.046LysXaa: 0.046 ± 0.046
Leu
6.288LeuAla: 6.288 ± 0.728
0.643LeuCys: 0.643 ± 0.193
6.242LeuAsp: 6.242 ± 0.453
7.343LeuGlu: 7.343 ± 0.533
3.121LeuPhe: 3.121 ± 0.386
5.462LeuGly: 5.462 ± 0.61
1.882LeuHis: 1.882 ± 0.312
5.37LeuIle: 5.37 ± 0.484
5.599LeuLys: 5.599 ± 0.613
6.242LeuLeu: 6.242 ± 0.5
2.111LeuMet: 2.111 ± 0.267
5.324LeuAsn: 5.324 ± 0.491
3.718LeuPro: 3.718 ± 0.506
3.855LeuGln: 3.855 ± 0.372
5.003LeuArg: 5.003 ± 0.372
6.517LeuSer: 6.517 ± 0.753
5.14LeuThr: 5.14 ± 0.405
5.324LeuVal: 5.324 ± 0.44
0.643LeuTrp: 0.643 ± 0.171
3.534LeuTyr: 3.534 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
1.239MetAla: 1.239 ± 0.309
0.184MetCys: 0.184 ± 0.096
1.239MetAsp: 1.239 ± 0.251
1.652MetGlu: 1.652 ± 0.28
0.459MetPhe: 0.459 ± 0.123
1.423MetGly: 1.423 ± 0.237
0.229MetHis: 0.229 ± 0.117
1.239MetIle: 1.239 ± 0.212
2.019MetLys: 2.019 ± 0.446
2.387MetLeu: 2.387 ± 0.337
0.734MetMet: 0.734 ± 0.153
1.836MetAsn: 1.836 ± 0.317
1.239MetPro: 1.239 ± 0.27
1.377MetGln: 1.377 ± 0.254
1.331MetArg: 1.331 ± 0.238
2.111MetSer: 2.111 ± 0.322
1.836MetThr: 1.836 ± 0.29
1.239MetVal: 1.239 ± 0.2
0.184MetTrp: 0.184 ± 0.097
0.826MetTyr: 0.826 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
4.314AsnAla: 4.314 ± 0.383
0.413AsnCys: 0.413 ± 0.167
2.983AsnAsp: 2.983 ± 0.338
3.35AsnGlu: 3.35 ± 0.426
2.019AsnPhe: 2.019 ± 0.277
3.442AsnGly: 3.442 ± 0.512
0.826AsnHis: 0.826 ± 0.275
3.947AsnIle: 3.947 ± 0.344
3.993AsnLys: 3.993 ± 0.486
6.288AsnLeu: 6.288 ± 0.72
1.515AsnMet: 1.515 ± 0.212
3.442AsnAsn: 3.442 ± 0.499
3.396AsnPro: 3.396 ± 0.358
2.983AsnGln: 2.983 ± 0.373
3.305AsnArg: 3.305 ± 0.535
3.488AsnSer: 3.488 ± 0.349
3.396AsnThr: 3.396 ± 0.395
3.029AsnVal: 3.029 ± 0.365
0.413AsnTrp: 0.413 ± 0.196
2.249AsnTyr: 2.249 ± 0.377
0.0AsnXaa: 0.0 ± 0.0
Pro
2.478ProAla: 2.478 ± 0.317
0.275ProCys: 0.275 ± 0.122
2.846ProAsp: 2.846 ± 0.289
5.875ProGlu: 5.875 ± 0.523
1.698ProPhe: 1.698 ± 0.378
2.708ProGly: 2.708 ± 0.278
0.78ProHis: 0.78 ± 0.191
2.708ProIle: 2.708 ± 0.327
2.019ProLys: 2.019 ± 0.31
3.396ProLeu: 3.396 ± 0.363
1.285ProMet: 1.285 ± 0.211
1.928ProAsn: 1.928 ± 0.303
1.239ProPro: 1.239 ± 0.245
1.423ProGln: 1.423 ± 0.214
1.836ProArg: 1.836 ± 0.307
2.662ProSer: 2.662 ± 0.326
2.295ProThr: 2.295 ± 0.31
3.488ProVal: 3.488 ± 0.375
0.046ProTrp: 0.046 ± 0.045
1.79ProTyr: 1.79 ± 0.396
0.0ProXaa: 0.0 ± 0.0
Gln
3.213GlnAla: 3.213 ± 0.453
0.229GlnCys: 0.229 ± 0.122
2.203GlnAsp: 2.203 ± 0.347
3.075GlnGlu: 3.075 ± 0.459
1.423GlnPhe: 1.423 ± 0.218
2.387GlnGly: 2.387 ± 0.388
0.643GlnHis: 0.643 ± 0.191
3.305GlnIle: 3.305 ± 0.323
2.754GlnLys: 2.754 ± 0.409
3.58GlnLeu: 3.58 ± 0.409
1.377GlnMet: 1.377 ± 0.207
2.203GlnAsn: 2.203 ± 0.327
1.606GlnPro: 1.606 ± 0.313
2.249GlnGln: 2.249 ± 0.314
1.606GlnArg: 1.606 ± 0.301
2.019GlnSer: 2.019 ± 0.329
1.882GlnThr: 1.882 ± 0.283
1.974GlnVal: 1.974 ± 0.309
0.229GlnTrp: 0.229 ± 0.116
1.147GlnTyr: 1.147 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
3.672ArgAla: 3.672 ± 0.55
0.413ArgCys: 0.413 ± 0.153
2.708ArgAsp: 2.708 ± 0.295
4.36ArgGlu: 4.36 ± 0.604
2.433ArgPhe: 2.433 ± 0.335
2.57ArgGly: 2.57 ± 0.312
0.459ArgHis: 0.459 ± 0.169
3.029ArgIle: 3.029 ± 0.347
3.534ArgLys: 3.534 ± 0.531
4.314ArgLeu: 4.314 ± 0.427
1.423ArgMet: 1.423 ± 0.252
3.075ArgAsn: 3.075 ± 0.395
1.423ArgPro: 1.423 ± 0.258
1.606ArgGln: 1.606 ± 0.319
2.157ArgArg: 2.157 ± 0.369
2.8ArgSer: 2.8 ± 0.468
2.754ArgThr: 2.754 ± 0.365
3.809ArgVal: 3.809 ± 0.53
0.505ArgTrp: 0.505 ± 0.163
1.698ArgTyr: 1.698 ± 0.303
0.0ArgXaa: 0.0 ± 0.0
Ser
3.901SerAla: 3.901 ± 0.588
0.413SerCys: 0.413 ± 0.165
3.809SerAsp: 3.809 ± 0.407
4.406SerGlu: 4.406 ± 0.452
2.387SerPhe: 2.387 ± 0.416
4.636SerGly: 4.636 ± 0.521
1.102SerHis: 1.102 ± 0.222
3.993SerIle: 3.993 ± 0.503
3.626SerLys: 3.626 ± 0.436
6.196SerLeu: 6.196 ± 0.469
1.836SerMet: 1.836 ± 0.288
4.085SerAsn: 4.085 ± 0.544
2.891SerPro: 2.891 ± 0.453
2.157SerGln: 2.157 ± 0.343
3.35SerArg: 3.35 ± 0.343
4.773SerSer: 4.773 ± 0.564
4.039SerThr: 4.039 ± 0.612
5.049SerVal: 5.049 ± 0.388
0.964SerTrp: 0.964 ± 0.253
2.433SerTyr: 2.433 ± 0.417
0.0SerXaa: 0.0 ± 0.0
Thr
3.718ThrAla: 3.718 ± 0.551
0.275ThrCys: 0.275 ± 0.116
3.626ThrAsp: 3.626 ± 0.442
4.406ThrGlu: 4.406 ± 0.366
2.157ThrPhe: 2.157 ± 0.242
4.177ThrGly: 4.177 ± 0.348
1.469ThrHis: 1.469 ± 0.272
3.855ThrIle: 3.855 ± 0.326
3.075ThrLys: 3.075 ± 0.353
4.406ThrLeu: 4.406 ± 0.438
1.147ThrMet: 1.147 ± 0.24
2.891ThrAsn: 2.891 ± 0.357
2.846ThrPro: 2.846 ± 0.311
1.698ThrGln: 1.698 ± 0.296
3.213ThrArg: 3.213 ± 0.38
3.809ThrSer: 3.809 ± 0.421
2.983ThrThr: 2.983 ± 0.425
3.947ThrVal: 3.947 ± 0.333
0.826ThrTrp: 0.826 ± 0.207
2.295ThrTyr: 2.295 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
4.636ValAla: 4.636 ± 0.459
0.688ValCys: 0.688 ± 0.256
3.947ValAsp: 3.947 ± 0.478
4.314ValGlu: 4.314 ± 0.404
2.754ValPhe: 2.754 ± 0.397
3.993ValGly: 3.993 ± 0.458
1.147ValHis: 1.147 ± 0.29
4.636ValIle: 4.636 ± 0.515
3.58ValLys: 3.58 ± 0.452
5.737ValLeu: 5.737 ± 0.419
1.285ValMet: 1.285 ± 0.239
4.223ValAsn: 4.223 ± 0.378
3.121ValPro: 3.121 ± 0.326
2.295ValGln: 2.295 ± 0.305
2.387ValArg: 2.387 ± 0.391
3.947ValSer: 3.947 ± 0.345
3.993ValThr: 3.993 ± 0.311
4.085ValVal: 4.085 ± 0.502
0.734ValTrp: 0.734 ± 0.212
2.249ValTyr: 2.249 ± 0.333
0.046ValXaa: 0.046 ± 0.049
Trp
0.459TrpAla: 0.459 ± 0.143
0.138TrpCys: 0.138 ± 0.087
0.872TrpAsp: 0.872 ± 0.225
1.056TrpGlu: 1.056 ± 0.21
0.643TrpPhe: 0.643 ± 0.177
0.918TrpGly: 0.918 ± 0.283
0.184TrpHis: 0.184 ± 0.09
0.597TrpIle: 0.597 ± 0.205
0.826TrpLys: 0.826 ± 0.253
1.056TrpLeu: 1.056 ± 0.307
0.321TrpMet: 0.321 ± 0.11
0.78TrpAsn: 0.78 ± 0.186
0.046TrpPro: 0.046 ± 0.042
0.597TrpGln: 0.597 ± 0.162
0.505TrpArg: 0.505 ± 0.153
0.734TrpSer: 0.734 ± 0.194
0.643TrpThr: 0.643 ± 0.181
0.78TrpVal: 0.78 ± 0.182
0.138TrpTrp: 0.138 ± 0.086
0.275TrpTyr: 0.275 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.928TyrAla: 1.928 ± 0.343
0.643TyrCys: 0.643 ± 0.253
2.387TyrAsp: 2.387 ± 0.31
2.57TyrGlu: 2.57 ± 0.353
1.147TyrPhe: 1.147 ± 0.296
2.387TyrGly: 2.387 ± 0.39
0.551TyrHis: 0.551 ± 0.153
2.937TyrIle: 2.937 ± 0.438
2.57TyrLys: 2.57 ± 0.445
3.534TyrLeu: 3.534 ± 0.425
0.78TyrMet: 0.78 ± 0.191
2.111TyrAsn: 2.111 ± 0.326
1.56TyrPro: 1.56 ± 0.35
1.515TyrGln: 1.515 ± 0.267
1.836TyrArg: 1.836 ± 0.252
2.662TyrSer: 2.662 ± 0.355
1.744TyrThr: 1.744 ± 0.308
2.983TyrVal: 2.983 ± 0.431
0.459TyrTrp: 0.459 ± 0.16
1.147TyrTyr: 1.147 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.046XaaPhe: 0.046 ± 0.05
0.046XaaGly: 0.046 ± 0.05
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.046XaaMet: 0.046 ± 0.049
0.046XaaAsn: 0.046 ± 0.045
0.0XaaPro: 0.0 ± 0.0
0.092XaaGln: 0.092 ± 0.061
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (21789 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski