Thermobifida phage P1312

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R8V9X2|A0A0R8V9X2_9CAUD Transposase OS=Thermobifida phage P1312 OX=1661715 GN=P1312_049 PE=3 SV=1
MM1 pKa = 7.95EE2 pKa = 6.29DD3 pKa = 4.22LMHH6 pKa = 7.29PPYY9 pKa = 10.08QQPPHH14 pKa = 6.8SSPVRR19 pKa = 11.84QQGMSAGAKK28 pKa = 9.24VALAGCGGCGALIGLVLMAGCVAAIVAPADD58 pKa = 3.67PAPPAAEE65 pKa = 4.13SSASGSASPEE75 pKa = 3.92EE76 pKa = 4.47SVSPSPEE83 pKa = 3.73PSEE86 pKa = 4.16TPEE89 pKa = 4.52DD90 pKa = 3.59EE91 pKa = 4.65VSFEE95 pKa = 4.18EE96 pKa = 5.51LGFPPALEE104 pKa = 4.37GEE106 pKa = 4.16EE107 pKa = 3.9RR108 pKa = 11.84AAYY111 pKa = 9.76LADD114 pKa = 3.76VRR116 pKa = 11.84DD117 pKa = 3.81LDD119 pKa = 4.01RR120 pKa = 11.84GFVFPDD126 pKa = 3.21EE127 pKa = 4.89DD128 pKa = 4.04SVVARR133 pKa = 11.84GQDD136 pKa = 3.33VCVTLRR142 pKa = 11.84DD143 pKa = 3.74EE144 pKa = 4.97GEE146 pKa = 3.98QAAIDD151 pKa = 4.14YY152 pKa = 10.84IEE154 pKa = 5.64AIWLHH159 pKa = 6.11EE160 pKa = 4.91DD161 pKa = 3.27YY162 pKa = 11.4DD163 pKa = 4.06FGRR166 pKa = 11.84RR167 pKa = 11.84PTTRR171 pKa = 11.84EE172 pKa = 3.46EE173 pKa = 4.06AEE175 pKa = 3.89QLLGIVHH182 pKa = 6.36EE183 pKa = 4.95HH184 pKa = 6.18VCPDD188 pKa = 2.91WW189 pKa = 5.83

Molecular weight: 20.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R8V9Y8|A0A0R8V9Y8_9CAUD Uncharacterized protein OS=Thermobifida phage P1312 OX=1661715 GN=P1312_070 PE=4 SV=1
MM1 pKa = 7.09AQLVKK6 pKa = 10.26RR7 pKa = 11.84AYY9 pKa = 9.65KK10 pKa = 9.99YY11 pKa = 10.34RR12 pKa = 11.84FYY14 pKa = 10.03PTLEE18 pKa = 3.86QAEE21 pKa = 4.28EE22 pKa = 3.92LLRR25 pKa = 11.84TFGCVRR31 pKa = 11.84FVYY34 pKa = 10.57NKK36 pKa = 10.2ALEE39 pKa = 4.22EE40 pKa = 3.85RR41 pKa = 11.84TRR43 pKa = 11.84AYY45 pKa = 8.09TQEE48 pKa = 3.78GRR50 pKa = 11.84RR51 pKa = 11.84VSYY54 pKa = 11.28VEE56 pKa = 3.74TSAALTQWKK65 pKa = 7.57RR66 pKa = 11.84TPEE69 pKa = 3.81LAFLNEE75 pKa = 4.21VSSVPLQQALRR86 pKa = 11.84HH87 pKa = 5.33LQAAFANFFAKK98 pKa = 9.81RR99 pKa = 11.84AKK101 pKa = 10.71YY102 pKa = 7.94PTFKK106 pKa = 10.55SKK108 pKa = 10.61KK109 pKa = 8.24KK110 pKa = 9.6SRR112 pKa = 11.84ASAEE116 pKa = 4.02YY117 pKa = 9.36TRR119 pKa = 11.84SAFRR123 pKa = 11.84WKK125 pKa = 10.52DD126 pKa = 3.14GRR128 pKa = 11.84LFLAKK133 pKa = 9.3MRR135 pKa = 11.84EE136 pKa = 4.03PLRR139 pKa = 11.84IVWSRR144 pKa = 11.84PLPEE148 pKa = 4.62GAQPSTVTVSRR159 pKa = 11.84DD160 pKa = 2.95AAGRR164 pKa = 11.84WFVSILVEE172 pKa = 4.19EE173 pKa = 5.31KK174 pKa = 10.22IRR176 pKa = 11.84PLPPVEE182 pKa = 4.27SSVGVDD188 pKa = 3.17AGISALVTLSTGEE201 pKa = 4.43KK202 pKa = 7.83ITNPGHH208 pKa = 5.35EE209 pKa = 3.83RR210 pKa = 11.84RR211 pKa = 11.84DD212 pKa = 3.47RR213 pKa = 11.84RR214 pKa = 11.84KK215 pKa = 9.38LAKK218 pKa = 9.84AQRR221 pKa = 11.84ALARR225 pKa = 11.84KK226 pKa = 9.19QKK228 pKa = 10.31GSKK231 pKa = 9.4NRR233 pKa = 11.84EE234 pKa = 3.58KK235 pKa = 11.08ARR237 pKa = 11.84LKK239 pKa = 9.93VARR242 pKa = 11.84IYY244 pKa = 11.39ARR246 pKa = 11.84ITDD249 pKa = 3.45RR250 pKa = 11.84RR251 pKa = 11.84RR252 pKa = 11.84DD253 pKa = 3.82FLHH256 pKa = 6.95KK257 pKa = 10.01LTTRR261 pKa = 11.84LVRR264 pKa = 11.84EE265 pKa = 4.05NQTVVIEE272 pKa = 4.37DD273 pKa = 3.43LTVRR277 pKa = 11.84NMLRR281 pKa = 11.84NHH283 pKa = 6.08SLARR287 pKa = 11.84AISDD291 pKa = 3.42ASWRR295 pKa = 11.84EE296 pKa = 3.61FRR298 pKa = 11.84SMLEE302 pKa = 4.25YY303 pKa = 10.53KK304 pKa = 10.23CAWYY308 pKa = 9.71DD309 pKa = 3.62RR310 pKa = 11.84EE311 pKa = 4.16LLVVDD316 pKa = 4.28RR317 pKa = 11.84WFPSSKK323 pKa = 10.31LCSACGTLQEE333 pKa = 4.58KK334 pKa = 9.8MPLNVRR340 pKa = 11.84TWQCACGAAHH350 pKa = 7.24DD351 pKa = 4.46RR352 pKa = 11.84DD353 pKa = 3.87VNAARR358 pKa = 11.84NILAAGLAEE367 pKa = 4.06RR368 pKa = 4.91

Molecular weight: 42.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

21268

75

1657

276.2

30.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.327 ± 0.438

0.987 ± 0.131

5.694 ± 0.206

6.503 ± 0.28

2.069 ± 0.231

9.216 ± 0.48

2.708 ± 0.252

3.399 ± 0.178

2.285 ± 0.19

8.83 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.655 ± 0.101

1.914 ± 0.129

6.446 ± 0.316

3.682 ± 0.161

10.001 ± 0.457

5.106 ± 0.266

5.99 ± 0.289

8.195 ± 0.283

1.994 ± 0.162

1.998 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski