Rutstroemia sp. NJR-2017a BBW

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Rutstroemiaceae; Rutstroemia; unclassified Rutstroemia

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7PF64|A0A2S7PF64_9HELO Uncharacterized protein OS=Rutstroemia sp. NJR-2017a BBW OX=2070414 GN=CJF30_00002829 PE=4 SV=1
MM1 pKa = 7.76SDD3 pKa = 4.24FAPPSGPPPPKK14 pKa = 10.39VPAGYY19 pKa = 9.74KK20 pKa = 10.17AVWNDD25 pKa = 3.49QYY27 pKa = 12.14NEE29 pKa = 3.64WFYY32 pKa = 11.95VNIYY36 pKa = 7.43TKK38 pKa = 10.66KK39 pKa = 9.12STWDD43 pKa = 3.24KK44 pKa = 9.15PTEE47 pKa = 4.03PVYY50 pKa = 10.5PPSDD54 pKa = 3.7EE55 pKa = 4.86APPAGPPPSYY65 pKa = 10.74GPSGSNSHH73 pKa = 6.34SPYY76 pKa = 9.5PTDD79 pKa = 3.47TKK81 pKa = 11.24SNPYY85 pKa = 8.37DD86 pKa = 3.36THH88 pKa = 6.6PSNNVEE94 pKa = 3.85SDD96 pKa = 3.03AALAARR102 pKa = 11.84LQAEE106 pKa = 3.96EE107 pKa = 4.29DD108 pKa = 3.49ARR110 pKa = 11.84AGSSSSGNRR119 pKa = 11.84NAMQDD124 pKa = 3.77YY125 pKa = 10.46QNTPMPQGSGSSSIQDD141 pKa = 3.3HH142 pKa = 6.23DD143 pKa = 3.8QEE145 pKa = 5.14EE146 pKa = 4.6YY147 pKa = 9.35QQGYY151 pKa = 9.33DD152 pKa = 4.25AGQNDD157 pKa = 4.25DD158 pKa = 4.61GGDD161 pKa = 3.41FGGDD165 pKa = 3.36DD166 pKa = 4.09GGDD169 pKa = 3.33FF170 pKa = 4.33

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7PJT5|A0A2S7PJT5_9HELO Ubiquitin carboxyl-terminal hydrolase family 1 protein OS=Rutstroemia sp. NJR-2017a BBW OX=2070414 GN=CJF30_00007136 PE=4 SV=1
MM1 pKa = 7.36GVRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 10.15KK7 pKa = 10.58LLRR10 pKa = 11.84QSRR13 pKa = 11.84LRR15 pKa = 11.84PLIHH19 pKa = 6.71HH20 pKa = 7.18RR21 pKa = 11.84SPHH24 pKa = 4.24QRR26 pKa = 11.84KK27 pKa = 8.76ARR29 pKa = 11.84APARR33 pKa = 3.79

Molecular weight: 4.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10779

0

10779

4847628

8

6812

449.7

49.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.227 ± 0.021

1.215 ± 0.01

5.5 ± 0.017

6.393 ± 0.026

3.746 ± 0.015

7.015 ± 0.022

2.272 ± 0.011

5.31 ± 0.019

5.251 ± 0.02

8.88 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.009

3.911 ± 0.012

5.71 ± 0.025

3.75 ± 0.015

5.701 ± 0.021

8.381 ± 0.031

6.102 ± 0.016

6.056 ± 0.015

1.463 ± 0.01

2.89 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski