Mycoplasma todarodis
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 894 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R0XN55|A0A4R0XN55_9MOLU Lipoprotein signal peptidase OS=Mycoplasma todarodis OX=1937191 GN=C4B25_02755 PE=3 SV=1
MM1 pKa = 7.55 EE2 pKa = 4.54 VPEE5 pKa = 5.13 FIQDD9 pKa = 3.69 FLDD12 pKa = 3.82 LVGNALLGIITGKK25 pKa = 6.28 YY26 pKa = 6.58 TCYY29 pKa = 9.81 NAPGIYY35 pKa = 9.84 YY36 pKa = 10.11 DD37 pKa = 5.58 LSSLDD42 pKa = 4.37 LDD44 pKa = 4.06 YY45 pKa = 11.38 LSPEE49 pKa = 3.38 IFKK52 pKa = 11.33 YY53 pKa = 9.76 MDD55 pKa = 3.13 YY56 pKa = 10.85 EE57 pKa = 4.38 GRR59 pKa = 11.84 DD60 pKa = 3.48 VQINLILDD68 pKa = 3.5 NKK70 pKa = 9.77 FDD72 pKa = 4.12 KK73 pKa = 10.22 FTLVCYY79 pKa = 10.14 IQRR82 pKa = 11.84 FRR84 pKa = 11.84 VV85 pKa = 3.3
Molecular weight: 9.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.977
IPC2_protein 4.202
IPC_protein 4.113
Toseland 3.897
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.266
Wikipedia 4.062
Rodwell 3.935
Grimsley 3.821
Solomon 4.088
Lehninger 4.05
Nozaki 4.228
DTASelect 4.469
Thurlkill 3.961
EMBOSS 4.062
Sillero 4.228
Patrickios 0.604
IPC_peptide 4.088
IPC2_peptide 4.202
IPC2.peptide.svr19 4.127
Protein with the highest isoelectric point:
>tr|A0A4R0XQE9|A0A4R0XQE9_9MOLU Sulfatase domain-containing protein OS=Mycoplasma todarodis OX=1937191 GN=C4B25_00595 PE=4 SV=1
MM1 pKa = 7.23 GVKK4 pKa = 10.36 KK5 pKa = 10.06 IRR7 pKa = 11.84 KK8 pKa = 7.99 KK9 pKa = 9.74 RR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 4.85 GFRR15 pKa = 11.84 ARR17 pKa = 11.84 MSSVGGRR24 pKa = 11.84 LVLKK28 pKa = 10.34 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 AKK33 pKa = 10.14 GRR35 pKa = 11.84 KK36 pKa = 8.18 QLTVSDD42 pKa = 4.08 KK43 pKa = 11.41
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
894
0
894
290648
21
3377
325.1
36.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.489 ± 0.157
0.42 ± 0.015
5.167 ± 0.07
7.153 ± 0.091
4.818 ± 0.064
5.899 ± 0.092
1.657 ± 0.031
9.215 ± 0.095
10.623 ± 0.11
8.324 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.625 ± 0.041
5.952 ± 0.078
2.827 ± 0.042
2.883 ± 0.033
3.23 ± 0.052
6.189 ± 0.057
5.879 ± 0.06
6.255 ± 0.063
1.119 ± 0.033
3.275 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here