Escherichia phage vojen
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9WNS7|A0A6B9WNS7_9CAUD Uncharacterized protein OS=Escherichia phage vojen OX=2696460 GN=vojen_4 PE=4 SV=1
MM1 pKa = 7.0 SQDD4 pKa = 3.09 GFFEE8 pKa = 4.19 RR9 pKa = 11.84 LQMAEE14 pKa = 3.8 DD15 pKa = 3.53 AGLNKK20 pKa = 8.58 EE21 pKa = 4.25 TALEE25 pKa = 3.89 VAYY28 pKa = 9.67 KK29 pKa = 10.27 IRR31 pKa = 11.84 TLDD34 pKa = 3.72 DD35 pKa = 3.96 ALGEE39 pKa = 3.83 MDD41 pKa = 4.5 MDD43 pKa = 4.56 HH44 pKa = 6.84 EE45 pKa = 5.04 CGAAFADD52 pKa = 3.6 PTMIVNDD59 pKa = 4.6 CGCNFDD65 pKa = 4.9 PNCPRR70 pKa = 11.84 CFPFF74 pKa = 5.73
Molecular weight: 8.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.973
IPC_protein 3.897
Toseland 3.694
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.846
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A6B9WNI4|A0A6B9WNI4_9CAUD Terminase small subunit OS=Escherichia phage vojen OX=2696460 GN=vojen_27 PE=4 SV=1
MM1 pKa = 7.83 LEE3 pKa = 4.22 LNDD6 pKa = 3.59 IRR8 pKa = 11.84 VGTKK12 pKa = 9.8 FRR14 pKa = 11.84 VTWVYY19 pKa = 11.02 DD20 pKa = 3.64 EE21 pKa = 4.54 RR22 pKa = 11.84 CGIRR26 pKa = 11.84 KK27 pKa = 9.94 GEE29 pKa = 4.08 IVTASDD35 pKa = 4.04 RR36 pKa = 11.84 YY37 pKa = 9.95 IGSLKK42 pKa = 10.18 EE43 pKa = 3.88 FNRR46 pKa = 11.84 PRR48 pKa = 11.84 IKK50 pKa = 10.36 NGYY53 pKa = 9.12 IITRR57 pKa = 11.84 RR58 pKa = 11.84 LGFDD62 pKa = 3.72 HH63 pKa = 6.74 YY64 pKa = 11.16 CVVATQGVLIEE75 pKa = 4.44 LKK77 pKa = 10.31 RR78 pKa = 11.84 ISDD81 pKa = 3.56 HH82 pKa = 6.68 RR83 pKa = 11.84 GCHH86 pKa = 4.82 VKK88 pKa = 9.67 TMKK91 pKa = 10.4 TPFQRR96 pKa = 11.84 ARR98 pKa = 11.84 YY99 pKa = 7.91 DD100 pKa = 3.15 ARR102 pKa = 11.84 RR103 pKa = 11.84 MRR105 pKa = 11.84 RR106 pKa = 11.84 LARR109 pKa = 11.84 NAIKK113 pKa = 10.19 FKK115 pKa = 11.06 KK116 pKa = 8.9 PDD118 pKa = 3.27 NVLYY122 pKa = 10.95 GMYY125 pKa = 10.25 KK126 pKa = 10.52 GIARR130 pKa = 11.84 NAGKK134 pKa = 10.28
Molecular weight: 15.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.823
IPC_protein 10.57
Toseland 10.701
ProMoST 10.409
Dawson 10.804
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.082
Grimsley 10.862
Solomon 10.891
Lehninger 10.862
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 10.789
IPC_peptide 10.906
IPC2_peptide 9.502
IPC2.peptide.svr19 8.491
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
15076
53
1141
188.4
21.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.079 ± 0.458
1.4 ± 0.167
6.282 ± 0.288
6.746 ± 0.287
3.88 ± 0.158
7.568 ± 0.26
1.864 ± 0.215
6.593 ± 0.178
7.409 ± 0.365
6.573 ± 0.189
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.237 ± 0.173
4.663 ± 0.207
3.429 ± 0.206
3.88 ± 0.294
4.988 ± 0.21
5.983 ± 0.264
5.32 ± 0.264
6.806 ± 0.216
1.453 ± 0.118
3.847 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here