Escherichia phage vojen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Hanrivervirus; Escherichia virus vojen

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6B9WNS7|A0A6B9WNS7_9CAUD Uncharacterized protein OS=Escherichia phage vojen OX=2696460 GN=vojen_4 PE=4 SV=1
MM1 pKa = 7.0SQDD4 pKa = 3.09GFFEE8 pKa = 4.19RR9 pKa = 11.84LQMAEE14 pKa = 3.8DD15 pKa = 3.53AGLNKK20 pKa = 8.58EE21 pKa = 4.25TALEE25 pKa = 3.89VAYY28 pKa = 9.67KK29 pKa = 10.27IRR31 pKa = 11.84TLDD34 pKa = 3.72DD35 pKa = 3.96ALGEE39 pKa = 3.83MDD41 pKa = 4.5MDD43 pKa = 4.56HH44 pKa = 6.84EE45 pKa = 5.04CGAAFADD52 pKa = 3.6PTMIVNDD59 pKa = 4.6CGCNFDD65 pKa = 4.9PNCPRR70 pKa = 11.84CFPFF74 pKa = 5.73

Molecular weight:
8.24 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6B9WNI4|A0A6B9WNI4_9CAUD Terminase small subunit OS=Escherichia phage vojen OX=2696460 GN=vojen_27 PE=4 SV=1
MM1 pKa = 7.83LEE3 pKa = 4.22LNDD6 pKa = 3.59IRR8 pKa = 11.84VGTKK12 pKa = 9.8FRR14 pKa = 11.84VTWVYY19 pKa = 11.02DD20 pKa = 3.64EE21 pKa = 4.54RR22 pKa = 11.84CGIRR26 pKa = 11.84KK27 pKa = 9.94GEE29 pKa = 4.08IVTASDD35 pKa = 4.04RR36 pKa = 11.84YY37 pKa = 9.95IGSLKK42 pKa = 10.18EE43 pKa = 3.88FNRR46 pKa = 11.84PRR48 pKa = 11.84IKK50 pKa = 10.36NGYY53 pKa = 9.12IITRR57 pKa = 11.84RR58 pKa = 11.84LGFDD62 pKa = 3.72HH63 pKa = 6.74YY64 pKa = 11.16CVVATQGVLIEE75 pKa = 4.44LKK77 pKa = 10.31RR78 pKa = 11.84ISDD81 pKa = 3.56HH82 pKa = 6.68RR83 pKa = 11.84GCHH86 pKa = 4.82VKK88 pKa = 9.67TMKK91 pKa = 10.4TPFQRR96 pKa = 11.84ARR98 pKa = 11.84YY99 pKa = 7.91DD100 pKa = 3.15ARR102 pKa = 11.84RR103 pKa = 11.84MRR105 pKa = 11.84RR106 pKa = 11.84LARR109 pKa = 11.84NAIKK113 pKa = 10.19FKK115 pKa = 11.06KK116 pKa = 8.9PDD118 pKa = 3.27NVLYY122 pKa = 10.95GMYY125 pKa = 10.25KK126 pKa = 10.52GIARR130 pKa = 11.84NAGKK134 pKa = 10.28

Molecular weight:
15.72 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15076

53

1141

188.4

21.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.079 ± 0.458

1.4 ± 0.167

6.282 ± 0.288

6.746 ± 0.287

3.88 ± 0.158

7.568 ± 0.26

1.864 ± 0.215

6.593 ± 0.178

7.409 ± 0.365

6.573 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.237 ± 0.173

4.663 ± 0.207

3.429 ± 0.206

3.88 ± 0.294

4.988 ± 0.21

5.983 ± 0.264

5.32 ± 0.264

6.806 ± 0.216

1.453 ± 0.118

3.847 ± 0.158

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski