Clostridium sp. MSTE9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3478 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J0N6R8|J0N6R8_9CLOT Efflux transporter RND family MFP subunit OS=Clostridium sp. MSTE9 OX=1105031 GN=HMPREF1141_3061 PE=3 SV=1
MM1 pKa = 8.26DD2 pKa = 4.53ISQINSYY9 pKa = 10.88SSASTKK15 pKa = 10.23RR16 pKa = 11.84SSANSSSSLSIEE28 pKa = 4.01QFLSLLAAQLSNQDD42 pKa = 3.67VLNPTQDD49 pKa = 3.52TEE51 pKa = 5.07FVAQMAQFTSLQALEE66 pKa = 4.79NLNQYY71 pKa = 11.06ASYY74 pKa = 10.74QYY76 pKa = 11.0GSSLIGKK83 pKa = 8.42KK84 pKa = 10.48VSVAMYY90 pKa = 10.08DD91 pKa = 3.31STGKK95 pKa = 10.21YY96 pKa = 8.96VSDD99 pKa = 3.51MGVVTQADD107 pKa = 4.33YY108 pKa = 11.61SSGDD112 pKa = 3.2TTVTVNGRR120 pKa = 11.84SYY122 pKa = 11.34GIANIMEE129 pKa = 4.38VFNDD133 pKa = 3.49ASYY136 pKa = 11.6GSYY139 pKa = 10.34QYY141 pKa = 11.32AASLVGKK148 pKa = 9.37KK149 pKa = 8.76VQVTGYY155 pKa = 9.86DD156 pKa = 3.28VNGNVVEE163 pKa = 4.16NTGIVSSCTFASGEE177 pKa = 3.99AMVVVDD183 pKa = 4.02GQKK186 pKa = 8.83YY187 pKa = 6.98TLSAVRR193 pKa = 11.84QVLSQNADD201 pKa = 3.6PEE203 pKa = 4.38PTDD206 pKa = 4.51PEE208 pKa = 4.42TSDD211 pKa = 3.54DD212 pKa = 3.84TTVPDD217 pKa = 4.03TDD219 pKa = 4.0SSGSTDD225 pKa = 3.86PDD227 pKa = 3.81TEE229 pKa = 4.3GG230 pKa = 3.67

Molecular weight: 24.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J0MW05|J0MW05_9CLOT Uncharacterized protein OS=Clostridium sp. MSTE9 OX=1105031 GN=HMPREF1141_2091 PE=4 SV=1
MM1 pKa = 7.31GSSRR5 pKa = 11.84RR6 pKa = 11.84STLAIFACAVSLLLAALLALSLGAVRR32 pKa = 11.84LSLTEE37 pKa = 3.8LFYY40 pKa = 11.22GLKK43 pKa = 10.39EE44 pKa = 3.78IDD46 pKa = 3.83SPAGRR51 pKa = 11.84ILLHH55 pKa = 5.56VRR57 pKa = 11.84VPRR60 pKa = 11.84LLAAALAGAGLSVSGVLIQTVLQNPLASPGIIGVNAGAGLAVALCMALLPGVVLVLPFAAFLGALASVLLVYY132 pKa = 10.16GIARR136 pKa = 11.84RR137 pKa = 11.84AGASRR142 pKa = 11.84ITLVLSGVALSSLMTAGINTLVTVFPDD169 pKa = 2.86IMTGLRR175 pKa = 11.84DD176 pKa = 3.7FQNGGFEE183 pKa = 4.34GVSTGILRR191 pKa = 11.84PAGLVILFGLLVSLLFAGEE210 pKa = 4.34LDD212 pKa = 3.66VLGLGEE218 pKa = 4.23ITASSLGLPVRR229 pKa = 11.84FYY231 pKa = 11.42RR232 pKa = 11.84FLFLALAAALAGAAVSFAGLLGFVGLIVPHH262 pKa = 5.96MARR265 pKa = 11.84FLLKK269 pKa = 10.71GSGKK273 pKa = 10.23RR274 pKa = 11.84SFLLLSALLGAAFLILCDD292 pKa = 3.54TAARR296 pKa = 11.84TAFAPFEE303 pKa = 4.39LPVGILVAYY312 pKa = 10.28LGVPFFLWLLFRR324 pKa = 11.84EE325 pKa = 3.94RR326 pKa = 11.84RR327 pKa = 11.84KK328 pKa = 10.38RR329 pKa = 11.84HH330 pKa = 5.54DD331 pKa = 3.17

Molecular weight: 34.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3478

0

3478

987318

37

2204

283.9

31.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.729 ± 0.05

1.482 ± 0.018

5.045 ± 0.032

6.698 ± 0.045

4.136 ± 0.033

7.511 ± 0.043

1.791 ± 0.019

6.674 ± 0.037

5.37 ± 0.033

9.903 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.9 ± 0.022

3.732 ± 0.03

4.014 ± 0.03

3.845 ± 0.03

5.064 ± 0.041

6.372 ± 0.041

5.356 ± 0.034

7.095 ± 0.035

0.881 ± 0.013

3.403 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski