Klebsiella phage KP27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Slopekvirus; Klebsiella virus KP27

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7NR34|K7NR34_9CAUD Recombination endonuclease subunit OS=Klebsiella phage KP27 OX=1129147 GN=KP27_065 PE=4 SV=1
MM1 pKa = 7.35MNLDD5 pKa = 3.48FCKK8 pKa = 10.86AEE10 pKa = 4.03IKK12 pKa = 10.67ADD14 pKa = 3.78ADD16 pKa = 3.6EE17 pKa = 5.02EE18 pKa = 4.46EE19 pKa = 4.66PKK21 pKa = 10.41FDD23 pKa = 4.2SPHH26 pKa = 5.84SSRR29 pKa = 11.84WIVAVDD35 pKa = 3.36DD36 pKa = 4.18EE37 pKa = 5.63GYY39 pKa = 8.7VTVLSRR45 pKa = 11.84PFIHH49 pKa = 7.68DD50 pKa = 3.38AFFEE54 pKa = 4.47CGRR57 pKa = 11.84SAEE60 pKa = 4.86DD61 pKa = 2.84IGLPDD66 pKa = 4.08SVEE69 pKa = 4.18MPPGVYY75 pKa = 9.52EE76 pKa = 4.23WICGFEE82 pKa = 4.0QTTDD86 pKa = 2.87WEE88 pKa = 4.3TGYY91 pKa = 11.05VDD93 pKa = 3.11GWEE96 pKa = 4.47FYY98 pKa = 10.33PNEE101 pKa = 4.04STLLYY106 pKa = 9.89SWEE109 pKa = 3.95NGKK112 pKa = 10.18VNEE115 pKa = 4.75SGNQEE120 pKa = 4.11TQSDD124 pKa = 4.0NGSGSLL130 pKa = 4.04

Molecular weight: 14.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7NRB3|K7NRB3_9CAUD Uncharacterized protein OS=Klebsiella phage KP27 OX=1129147 GN=KP27_182 PE=4 SV=1
MM1 pKa = 7.15LVSMRR6 pKa = 11.84ALGLVTIAGMMTMFSAGYY24 pKa = 9.56FIGVVIGFIIFGGVLYY40 pKa = 9.62MCHH43 pKa = 6.25QEE45 pKa = 3.67DD46 pKa = 3.7RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 3.85RR51 pKa = 11.84KK52 pKa = 9.12ARR54 pKa = 11.84KK55 pKa = 8.97

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

276

0

276

55541

39

1357

201.2

22.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.7 ± 0.162

1.228 ± 0.069

6.57 ± 0.109

6.926 ± 0.167

4.114 ± 0.111

6.347 ± 0.2

1.755 ± 0.075

6.779 ± 0.121

7.353 ± 0.162

7.497 ± 0.13

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.074

5.524 ± 0.135

3.547 ± 0.089

3.239 ± 0.101

4.76 ± 0.116

6.077 ± 0.131

5.882 ± 0.221

6.962 ± 0.133

1.331 ± 0.053

4.474 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski