Firmicutes bacterium CAG:884

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1232 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5MRR5|R5MRR5_9FIRM NERD domain-containing protein OS=Firmicutes bacterium CAG:884 OX=1262990 GN=BN804_00599 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 8.81MTVDD6 pKa = 3.4KK7 pKa = 10.51NACIGCGACVGTCDD21 pKa = 4.64KK22 pKa = 11.38VFGFEE27 pKa = 4.14DD28 pKa = 3.54NYY30 pKa = 11.7AEE32 pKa = 4.46VIVDD36 pKa = 5.31EE37 pKa = 4.63IPEE40 pKa = 4.23DD41 pKa = 3.81LKK43 pKa = 11.32EE44 pKa = 4.23DD45 pKa = 3.98AMDD48 pKa = 4.27ALEE51 pKa = 4.72GCPVDD56 pKa = 4.96AISIVDD62 pKa = 3.49EE63 pKa = 4.45SS64 pKa = 3.67

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5MPF3|R5MPF3_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:884 OX=1262990 GN=BN804_00182 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 9.45GTYY5 pKa = 9.03QPNNRR10 pKa = 11.84KK11 pKa = 9.36RR12 pKa = 11.84SKK14 pKa = 10.57KK15 pKa = 9.71QGFLARR21 pKa = 11.84MKK23 pKa = 10.42TNVINKK29 pKa = 8.92RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 8.28GRR35 pKa = 11.84KK36 pKa = 8.67VLAHH40 pKa = 6.78

Molecular weight: 4.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1232

0

1232

363460

29

2672

295.0

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.229 ± 0.063

1.153 ± 0.03

5.887 ± 0.063

7.318 ± 0.084

4.272 ± 0.048

5.356 ± 0.091

1.199 ± 0.029

10.28 ± 0.094

10.19 ± 0.087

9.007 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.034

7.944 ± 0.085

2.425 ± 0.033

1.841 ± 0.031

3.105 ± 0.049

6.126 ± 0.081

5.245 ± 0.079

5.997 ± 0.06

0.534 ± 0.017

5.491 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski