Amino acid dipepetide frequency for Pleurotus ostreatus PC15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.397AlaAla: 8.397 ± 0.059	1.08AlaCys: 1.08 ± 0.016	4.172AlaAsp: 4.172 ± 0.029	4.759AlaGlu: 4.759 ± 0.035	3.328AlaPhe: 3.328 ± 0.028	5.429AlaGly: 5.429 ± 0.037	1.98AlaHis: 1.98 ± 0.022	4.298AlaIle: 4.298 ± 0.032	3.985AlaLys: 3.985 ± 0.03	8.152AlaLeu: 8.152 ± 0.05	1.863AlaMet: 1.863 ± 0.021	2.998AlaAsn: 2.998 ± 0.026	5.158AlaPro: 5.158 ± 0.049	3.262AlaGln: 3.262 ± 0.03	5.045AlaArg: 5.045 ± 0.035	7.623AlaSer: 7.623 ± 0.041	5.291AlaThr: 5.291 ± 0.037	5.239AlaVal: 5.239 ± 0.035	1.17AlaTrp: 1.17 ± 0.016	2.244AlaTyr: 2.244 ± 0.023	0.0AlaXaa: 0.0 ± 0.0
Cys	0.999CysAla: 0.999 ± 0.016	0.26CysCys: 0.26 ± 0.007	0.663CysAsp: 0.663 ± 0.012	0.584CysGlu: 0.584 ± 0.012	0.525CysPhe: 0.525 ± 0.01	0.925CysGly: 0.925 ± 0.016	0.333CysHis: 0.333 ± 0.008	0.718CysIle: 0.718 ± 0.013	0.502CysLys: 0.502 ± 0.01	1.259CysLeu: 1.259 ± 0.018	0.26CysMet: 0.26 ± 0.007	0.444CysAsn: 0.444 ± 0.011	0.779CysPro: 0.779 ± 0.015	0.435CysGln: 0.435 ± 0.01	0.753CysArg: 0.753 ± 0.012	0.987CysSer: 0.987 ± 0.017	0.747CysThr: 0.747 ± 0.014	0.81CysVal: 0.81 ± 0.012	0.196CysTrp: 0.196 ± 0.007	0.329CysTyr: 0.329 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.994AspAla: 4.994 ± 0.037	0.609AspCys: 0.609 ± 0.011	4.387AspAsp: 4.387 ± 0.041	4.306AspGlu: 4.306 ± 0.037	2.153AspPhe: 2.153 ± 0.02	4.119AspGly: 4.119 ± 0.028	1.281AspHis: 1.281 ± 0.017	3.268AspIle: 3.268 ± 0.026	2.263AspLys: 2.263 ± 0.022	4.914AspLeu: 4.914 ± 0.035	1.185AspMet: 1.185 ± 0.016	1.882AspAsn: 1.882 ± 0.021	3.457AspPro: 3.457 ± 0.028	1.617AspGln: 1.617 ± 0.019	2.777AspArg: 2.777 ± 0.024	4.156AspSer: 4.156 ± 0.031	3.061AspThr: 3.061 ± 0.022	4.042AspVal: 4.042 ± 0.03	0.894AspTrp: 0.894 ± 0.014	1.52AspTyr: 1.52 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.03GluAla: 5.03 ± 0.041	0.669GluCys: 0.669 ± 0.012	4.047GluAsp: 4.047 ± 0.039	5.013GluGlu: 5.013 ± 0.049	1.897GluPhe: 1.897 ± 0.019	3.698GluGly: 3.698 ± 0.031	1.412GluHis: 1.412 ± 0.019	2.823GluIle: 2.823 ± 0.026	2.947GluLys: 2.947 ± 0.028	5.167GluLeu: 5.167 ± 0.038	1.299GluMet: 1.299 ± 0.016	1.826GluAsn: 1.826 ± 0.022	2.638GluPro: 2.638 ± 0.026	2.085GluGln: 2.085 ± 0.021	3.796GluArg: 3.796 ± 0.032	3.88GluSer: 3.88 ± 0.031	2.992GluThr: 2.992 ± 0.025	3.605GluVal: 3.605 ± 0.032	0.923GluTrp: 0.923 ± 0.014	1.628GluTyr: 1.628 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.225PheAla: 3.225 ± 0.029	0.54PheCys: 0.54 ± 0.012	2.412PheAsp: 2.412 ± 0.022	1.975PheGlu: 1.975 ± 0.017	1.601PhePhe: 1.601 ± 0.021	2.73PheGly: 2.73 ± 0.028	0.983PheHis: 0.983 ± 0.014	1.958PheIle: 1.958 ± 0.019	1.562PheLys: 1.562 ± 0.019	3.523PheLeu: 3.523 ± 0.029	0.78PheMet: 0.78 ± 0.013	1.465PheAsn: 1.465 ± 0.018	2.117PhePro: 2.117 ± 0.021	1.264PheGln: 1.264 ± 0.015	1.949PheArg: 1.949 ± 0.018	3.111PheSer: 3.111 ± 0.028	2.294PheThr: 2.294 ± 0.019	2.609PheVal: 2.609 ± 0.029	0.569PheTrp: 0.569 ± 0.012	1.07PheTyr: 1.07 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	5.108GlyAla: 5.108 ± 0.036	0.872GlyCys: 0.872 ± 0.016	3.663GlyAsp: 3.663 ± 0.028	3.454GlyGlu: 3.454 ± 0.028	2.606GlyPhe: 2.606 ± 0.028	5.547GlyGly: 5.547 ± 0.05	1.688GlyHis: 1.688 ± 0.018	3.394GlyIle: 3.394 ± 0.03	3.307GlyLys: 3.307 ± 0.027	5.73GlyLeu: 5.73 ± 0.041	1.505GlyMet: 1.505 ± 0.023	2.44GlyAsn: 2.44 ± 0.023	3.221GlyPro: 3.221 ± 0.03	2.231GlyGln: 2.231 ± 0.023	4.09GlyArg: 4.09 ± 0.032	5.603GlySer: 5.603 ± 0.045	3.904GlyThr: 3.904 ± 0.03	4.447GlyVal: 4.447 ± 0.034	1.123GlyTrp: 1.123 ± 0.015	1.882GlyTyr: 1.882 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	2.053HisAla: 2.053 ± 0.024	0.362HisCys: 0.362 ± 0.008	1.339HisAsp: 1.339 ± 0.017	1.265HisGlu: 1.265 ± 0.017	1.0HisPhe: 1.0 ± 0.012	1.697HisGly: 1.697 ± 0.021	0.894HisHis: 0.894 ± 0.016	1.382HisIle: 1.382 ± 0.018	0.912HisLys: 0.912 ± 0.012	2.509HisLeu: 2.509 ± 0.027	0.472HisMet: 0.472 ± 0.01	0.857HisAsn: 0.857 ± 0.014	1.937HisPro: 1.937 ± 0.022	0.929HisGln: 0.929 ± 0.015	1.637HisArg: 1.637 ± 0.018	2.105HisSer: 2.105 ± 0.023	1.486HisThr: 1.486 ± 0.018	1.618HisVal: 1.618 ± 0.02	0.363HisTrp: 0.363 ± 0.008	0.703HisTyr: 0.703 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.286IleAla: 4.286 ± 0.031	0.748IleCys: 0.748 ± 0.012	2.96IleAsp: 2.96 ± 0.025	2.657IleGlu: 2.657 ± 0.024	1.982IlePhe: 1.982 ± 0.022	3.042IleGly: 3.042 ± 0.03	1.343IleHis: 1.343 ± 0.016	2.629IleIle: 2.629 ± 0.024	2.148IleLys: 2.148 ± 0.023	4.761IleLeu: 4.761 ± 0.033	0.977IleMet: 0.977 ± 0.014	1.819IleAsn: 1.819 ± 0.021	3.418IlePro: 3.418 ± 0.025	1.911IleGln: 1.911 ± 0.022	2.973IleArg: 2.973 ± 0.027	4.066IleSer: 4.066 ± 0.032	2.968IleThr: 2.968 ± 0.027	3.419IleVal: 3.419 ± 0.027	0.759IleTrp: 0.759 ± 0.015	1.4IleTyr: 1.4 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.178LysAla: 4.178 ± 0.036	0.51LysCys: 0.51 ± 0.011	2.612LysAsp: 2.612 ± 0.025	3.116LysGlu: 3.116 ± 0.029	1.458LysPhe: 1.458 ± 0.019	2.934LysGly: 2.934 ± 0.026	1.158LysHis: 1.158 ± 0.016	2.095LysIle: 2.095 ± 0.02	2.911LysLys: 2.911 ± 0.036	3.988LysLeu: 3.988 ± 0.036	0.936LysMet: 0.936 ± 0.014	1.448LysAsn: 1.448 ± 0.019	2.604LysPro: 2.604 ± 0.028	1.683LysGln: 1.683 ± 0.017	3.373LysArg: 3.373 ± 0.03	3.258LysSer: 3.258 ± 0.027	2.55LysThr: 2.55 ± 0.023	2.836LysVal: 2.836 ± 0.025	0.688LysTrp: 0.688 ± 0.012	1.307LysTyr: 1.307 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	8.081LeuAla: 8.081 ± 0.044	1.242LeuCys: 1.242 ± 0.017	5.236LeuAsp: 5.236 ± 0.037	5.292LeuGlu: 5.292 ± 0.038	3.412LeuPhe: 3.412 ± 0.028	5.704LeuGly: 5.704 ± 0.042	2.461LeuHis: 2.461 ± 0.023	4.042LeuIle: 4.042 ± 0.032	4.158LeuLys: 4.158 ± 0.031	8.711LeuLeu: 8.711 ± 0.064	1.744LeuMet: 1.744 ± 0.022	3.19LeuAsn: 3.19 ± 0.029	6.301LeuPro: 6.301 ± 0.034	3.665LeuGln: 3.665 ± 0.031	6.011LeuArg: 6.011 ± 0.043	7.809LeuSer: 7.809 ± 0.045	5.178LeuThr: 5.178 ± 0.031	5.836LeuVal: 5.836 ± 0.037	1.229LeuTrp: 1.229 ± 0.015	2.43LeuTyr: 2.43 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	1.9MetAla: 1.9 ± 0.019	0.238MetCys: 0.238 ± 0.008	1.234MetAsp: 1.234 ± 0.018	1.158MetGlu: 1.158 ± 0.015	0.756MetPhe: 0.756 ± 0.01	1.34MetGly: 1.34 ± 0.019	0.495MetHis: 0.495 ± 0.01	0.957MetIle: 0.957 ± 0.015	1.004MetLys: 1.004 ± 0.014	1.797MetLeu: 1.797 ± 0.022	0.508MetMet: 0.508 ± 0.012	0.768MetAsn: 0.768 ± 0.014	1.282MetPro: 1.282 ± 0.015	0.783MetGln: 0.783 ± 0.013	1.227MetArg: 1.227 ± 0.017	1.926MetSer: 1.926 ± 0.019	1.218MetThr: 1.218 ± 0.015	1.248MetVal: 1.248 ± 0.016	0.264MetTrp: 0.264 ± 0.008	0.55MetTyr: 0.55 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.161AsnAla: 3.161 ± 0.024	0.403AsnCys: 0.403 ± 0.01	1.973AsnAsp: 1.973 ± 0.023	1.893AsnGlu: 1.893 ± 0.02	1.351AsnPhe: 1.351 ± 0.016	2.83AsnGly: 2.83 ± 0.029	0.892AsnHis: 0.892 ± 0.014	2.011AsnIle: 2.011 ± 0.02	1.396AsnLys: 1.396 ± 0.016	3.163AsnLeu: 3.163 ± 0.03	0.745AsnMet: 0.745 ± 0.013	1.434AsnAsn: 1.434 ± 0.022	2.465AsnPro: 2.465 ± 0.028	1.197AsnGln: 1.197 ± 0.018	1.763AsnArg: 1.763 ± 0.02	2.602AsnSer: 2.602 ± 0.024	2.231AsnThr: 2.231 ± 0.022	2.457AsnVal: 2.457 ± 0.024	0.506AsnTrp: 0.506 ± 0.01	0.973AsnTyr: 0.973 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	5.374ProAla: 5.374 ± 0.047	0.55ProCys: 0.55 ± 0.012	3.249ProAsp: 3.249 ± 0.027	3.453ProGlu: 3.453 ± 0.029	2.292ProPhe: 2.292 ± 0.024	3.785ProGly: 3.785 ± 0.033	1.613ProHis: 1.613 ± 0.019	2.882ProIle: 2.882 ± 0.023	2.6ProLys: 2.6 ± 0.029	5.443ProLeu: 5.443 ± 0.037	1.099ProMet: 1.099 ± 0.016	2.387ProAsn: 2.387 ± 0.024	6.519ProPro: 6.519 ± 0.075	2.385ProGln: 2.385 ± 0.026	3.615ProArg: 3.615 ± 0.033	7.258ProSer: 7.258 ± 0.057	4.545ProThr: 4.545 ± 0.036	3.764ProVal: 3.764 ± 0.032	0.719ProTrp: 0.719 ± 0.013	1.624ProTyr: 1.624 ± 0.019	0.0ProXaa: 0.0 ± 0.0
Gln	3.352GlnAla: 3.352 ± 0.031	0.489GlnCys: 0.489 ± 0.01	1.855GlnAsp: 1.855 ± 0.02	2.056GlnGlu: 2.056 ± 0.025	1.277GlnPhe: 1.277 ± 0.016	2.201GlnGly: 2.201 ± 0.02	1.006GlnHis: 1.006 ± 0.013	1.83GlnIle: 1.83 ± 0.02	1.579GlnLys: 1.579 ± 0.017	3.409GlnLeu: 3.409 ± 0.029	0.802GlnMet: 0.802 ± 0.012	1.259GlnAsn: 1.259 ± 0.015	2.368GlnPro: 2.368 ± 0.028	1.847GlnGln: 1.847 ± 0.032	2.498GlnArg: 2.498 ± 0.024	2.869GlnSer: 2.869 ± 0.024	2.219GlnThr: 2.219 ± 0.022	2.184GlnVal: 2.184 ± 0.023	0.578GlnTrp: 0.578 ± 0.012	1.083GlnTyr: 1.083 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	4.838ArgAla: 4.838 ± 0.033	0.776ArgCys: 0.776 ± 0.013	3.243ArgAsp: 3.243 ± 0.031	3.513ArgGlu: 3.513 ± 0.033	2.208ArgPhe: 2.208 ± 0.023	3.644ArgGly: 3.644 ± 0.03	1.638ArgHis: 1.638 ± 0.019	3.132ArgIle: 3.132 ± 0.026	3.252ArgLys: 3.252 ± 0.026	5.733ArgLeu: 5.733 ± 0.038	1.331ArgMet: 1.331 ± 0.016	2.172ArgAsn: 2.172 ± 0.021	3.578ArgPro: 3.578 ± 0.037	2.332ArgGln: 2.332 ± 0.023	5.108ArgArg: 5.108 ± 0.041	4.93ArgSer: 4.93 ± 0.036	3.508ArgThr: 3.508 ± 0.033	3.619ArgVal: 3.619 ± 0.032	0.95ArgTrp: 0.95 ± 0.012	1.652ArgTyr: 1.652 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	6.757SerAla: 6.757 ± 0.04	0.926SerCys: 0.926 ± 0.014	4.413SerAsp: 4.413 ± 0.033	3.964SerGlu: 3.964 ± 0.031	3.197SerPhe: 3.197 ± 0.028	5.45SerGly: 5.45 ± 0.043	2.069SerHis: 2.069 ± 0.023	4.173SerIle: 4.173 ± 0.03	3.702SerLys: 3.702 ± 0.031	7.817SerLeu: 7.817 ± 0.04	1.741SerMet: 1.741 ± 0.02	2.984SerAsn: 2.984 ± 0.027	6.38SerPro: 6.38 ± 0.058	3.113SerGln: 3.113 ± 0.03	5.039SerArg: 5.039 ± 0.038	9.494SerSer: 9.494 ± 0.083	6.105SerThr: 6.105 ± 0.042	4.944SerVal: 4.944 ± 0.038	1.099SerTrp: 1.099 ± 0.016	2.081SerTyr: 2.081 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	4.882ThrAla: 4.882 ± 0.034	0.75ThrCys: 0.75 ± 0.012	2.861ThrAsp: 2.861 ± 0.02	2.82ThrGlu: 2.82 ± 0.026	2.44ThrPhe: 2.44 ± 0.023	3.849ThrGly: 3.849 ± 0.029	1.488ThrHis: 1.488 ± 0.018	3.311ThrIle: 3.311 ± 0.027	2.545ThrLys: 2.545 ± 0.026	5.785ThrLeu: 5.785 ± 0.035	1.21ThrMet: 1.21 ± 0.016	2.145ThrAsn: 2.145 ± 0.022	4.822ThrPro: 4.822 ± 0.043	2.133ThrGln: 2.133 ± 0.025	3.363ThrArg: 3.363 ± 0.024	5.804ThrSer: 5.804 ± 0.042	4.35ThrThr: 4.35 ± 0.041	3.848ThrVal: 3.848 ± 0.031	0.843ThrTrp: 0.843 ± 0.013	1.603ThrTyr: 1.603 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.498ValAla: 5.498 ± 0.037	0.868ValCys: 0.868 ± 0.013	3.994ValAsp: 3.994 ± 0.03	3.833ValGlu: 3.833 ± 0.034	2.55ValPhe: 2.55 ± 0.02	4.089ValGly: 4.089 ± 0.032	1.645ValHis: 1.645 ± 0.02	3.156ValIle: 3.156 ± 0.027	2.945ValLys: 2.945 ± 0.027	5.989ValLeu: 5.989 ± 0.035	1.271ValMet: 1.271 ± 0.018	2.267ValAsn: 2.267 ± 0.022	3.918ValPro: 3.918 ± 0.032	2.362ValGln: 2.362 ± 0.025	3.559ValArg: 3.559 ± 0.026	4.813ValSer: 4.813 ± 0.033	3.576ValThr: 3.576 ± 0.029	4.679ValVal: 4.679 ± 0.035	0.953ValTrp: 0.953 ± 0.015	1.872ValTyr: 1.872 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.101TrpAla: 1.101 ± 0.015	0.221TrpCys: 0.221 ± 0.007	0.911TrpAsp: 0.911 ± 0.014	0.836TrpGlu: 0.836 ± 0.013	0.554TrpPhe: 0.554 ± 0.013	0.901TrpGly: 0.901 ± 0.015	0.362TrpHis: 0.362 ± 0.009	0.775TrpIle: 0.775 ± 0.013	0.825TrpLys: 0.825 ± 0.013	1.295TrpLeu: 1.295 ± 0.018	0.356TrpMet: 0.356 ± 0.008	0.613TrpAsn: 0.613 ± 0.013	0.602TrpPro: 0.602 ± 0.011	0.514TrpGln: 0.514 ± 0.009	1.005TrpArg: 1.005 ± 0.016	1.088TrpSer: 1.088 ± 0.015	0.912TrpThr: 0.912 ± 0.013	0.903TrpVal: 0.903 ± 0.014	0.269TrpTrp: 0.269 ± 0.009	0.429TrpTyr: 0.429 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.256TyrAla: 2.256 ± 0.022	0.395TyrCys: 0.395 ± 0.008	1.664TyrAsp: 1.664 ± 0.018	1.43TyrGlu: 1.43 ± 0.019	1.148TyrPhe: 1.148 ± 0.017	1.904TyrGly: 1.904 ± 0.025	0.777TyrHis: 0.777 ± 0.011	1.43TyrIle: 1.43 ± 0.016	1.1TyrLys: 1.1 ± 0.017	2.584TyrLeu: 2.584 ± 0.024	0.568TyrMet: 0.568 ± 0.011	1.049TyrAsn: 1.049 ± 0.016	1.59TyrPro: 1.59 ± 0.02	1.016TyrGln: 1.016 ± 0.015	1.578TyrArg: 1.578 ± 0.02	2.064TyrSer: 2.064 ± 0.022	1.718TyrThr: 1.718 ± 0.019	1.73TyrVal: 1.73 ± 0.02	0.377TyrTrp: 0.377 ± 0.008	0.887TyrTyr: 0.887 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 12186 proteins (5090183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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