Leifsonia sp. Root60

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; unclassified Leifsonia

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8CK64|A0A0Q8CK64_9MICO FAD:protein FMN transferase OS=Leifsonia sp. Root60 OX=1736567 GN=ASD61_08350 PE=3 SV=1
MM1 pKa = 7.51ISAAYY6 pKa = 9.63DD7 pKa = 3.38GAMPTRR13 pKa = 11.84TPTALVALPATVAIAAALALALSGCAGGSPAPSDD47 pKa = 3.66AASDD51 pKa = 4.17SPNPTPTFGPSTSSPASSSPVAPRR75 pKa = 11.84DD76 pKa = 3.19QSAFGTEE83 pKa = 4.07SPIYY87 pKa = 10.64APGCEE92 pKa = 3.86TFLTEE97 pKa = 4.27EE98 pKa = 3.98QTVALVDD105 pKa = 3.21QGYY108 pKa = 9.15VPDD111 pKa = 4.75PTSTWPDD118 pKa = 3.03VMAEE122 pKa = 3.79AAAAGGTWCAWTAPDD137 pKa = 4.64GSPDD141 pKa = 2.94IRR143 pKa = 11.84GGVVAIPEE151 pKa = 4.42ALWQAKK157 pKa = 10.07RR158 pKa = 11.84GDD160 pKa = 4.75LIAQGATEE168 pKa = 5.07DD169 pKa = 3.9DD170 pKa = 3.51ASRR173 pKa = 11.84PGFIALPDD181 pKa = 3.6EE182 pKa = 4.7GAPDD186 pKa = 3.46VDD188 pKa = 3.71GGFVYY193 pKa = 10.91ADD195 pKa = 3.83GYY197 pKa = 10.61LVYY200 pKa = 11.11VSDD203 pKa = 4.53PKK205 pKa = 10.78LAASVTVLQQ214 pKa = 4.48

Molecular weight: 21.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8CC78|A0A0Q8CC78_9MICO ABC transporter OS=Leifsonia sp. Root60 OX=1736567 GN=ASD61_07095 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3234

0

3234

1047850

29

3098

324.0

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.45 ± 0.057

0.458 ± 0.01

6.334 ± 0.039

5.467 ± 0.042

3.241 ± 0.024

8.911 ± 0.038

1.876 ± 0.018

4.896 ± 0.029

2.048 ± 0.034

10.047 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.732 ± 0.017

2.025 ± 0.024

5.267 ± 0.03

2.679 ± 0.023

6.809 ± 0.044

6.102 ± 0.034

6.156 ± 0.047

8.974 ± 0.04

1.502 ± 0.02

2.025 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski