Vesicular stomatitis virus
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A110ASW0|A0A110ASW0_9RHAB GDP polyribonucleotidyltransferase OS=Vesicular stomatitis virus OX=11276 PE=3 SV=1
MM1 pKa = 8.21 DD2 pKa = 5.84 SIDD5 pKa = 3.68 RR6 pKa = 11.84 LKK8 pKa = 10.32 TYY10 pKa = 10.46 LATYY14 pKa = 10.48 DD15 pKa = 4.05 NLDD18 pKa = 4.09 SALQDD23 pKa = 3.42 ANEE26 pKa = 4.16 SEE28 pKa = 4.3 EE29 pKa = 4.06 RR30 pKa = 11.84 RR31 pKa = 11.84 EE32 pKa = 4.2 DD33 pKa = 4.49 KK34 pKa = 10.96 YY35 pKa = 11.59 LQDD38 pKa = 5.98 LFIEE42 pKa = 4.58 DD43 pKa = 4.04 QGDD46 pKa = 3.64 KK47 pKa = 8.78 PTPSYY52 pKa = 10.19 YY53 pKa = 10.06 QEE55 pKa = 4.54 EE56 pKa = 4.47 EE57 pKa = 4.43 SSDD60 pKa = 3.45 SDD62 pKa = 3.11 TDD64 pKa = 3.66 YY65 pKa = 11.37 NAEE68 pKa = 4.28 HH69 pKa = 6.68 LTMLSPDD76 pKa = 3.2 EE77 pKa = 6.02 RR78 pKa = 11.84 IDD80 pKa = 3.24 KK81 pKa = 10.3 WEE83 pKa = 3.96 EE84 pKa = 3.59 DD85 pKa = 3.67 LPEE88 pKa = 4.77 LEE90 pKa = 5.82 KK91 pKa = 10.31 IDD93 pKa = 5.26 DD94 pKa = 5.31 DD95 pKa = 4.61 IPVTFSDD102 pKa = 3.17 WTQPVMKK109 pKa = 10.35 GNGGEE114 pKa = 4.13 KK115 pKa = 10.02 SLSLFPPVGLTRR127 pKa = 11.84 VQTDD131 pKa = 2.32 QWMKK135 pKa = 10.18 TIEE138 pKa = 4.15 AVCEE142 pKa = 4.01 SSKK145 pKa = 10.81 YY146 pKa = 9.41 WNLSEE151 pKa = 4.35 CRR153 pKa = 11.84 IMNSGNCLILKK164 pKa = 9.13 GRR166 pKa = 11.84 VMTPDD171 pKa = 3.78 CSSSTKK177 pKa = 8.59 SQKK180 pKa = 10.89 SMQSSEE186 pKa = 4.46 SLSSSHH192 pKa = 6.88 SPGPAPKK199 pKa = 8.9 VTEE202 pKa = 4.32 STSLWDD208 pKa = 3.81 LKK210 pKa = 10.34 STEE213 pKa = 3.93 VQLISKK219 pKa = 9.9 RR220 pKa = 11.84 AGVKK224 pKa = 10.79 DD225 pKa = 3.63 MMVKK229 pKa = 9.46 LTDD232 pKa = 4.01 FFRR235 pKa = 11.84 SEE237 pKa = 3.89 DD238 pKa = 3.69 EE239 pKa = 4.49 YY240 pKa = 11.79 YY241 pKa = 10.76 SVCPEE246 pKa = 4.24 GAPDD250 pKa = 3.98 LMGAIIMGLKK260 pKa = 9.59 HH261 pKa = 6.63 KK262 pKa = 10.68 KK263 pKa = 10.02 LFNQARR269 pKa = 11.84 MKK271 pKa = 10.8 YY272 pKa = 9.76 RR273 pKa = 11.84 LL274 pKa = 3.43
Molecular weight: 31.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.615
IPC2_protein 4.571
IPC_protein 4.52
Toseland 4.355
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.622
Wikipedia 4.355
Rodwell 4.355
Grimsley 4.266
Solomon 4.469
Lehninger 4.431
Nozaki 4.584
DTASelect 4.762
Thurlkill 4.368
EMBOSS 4.368
Sillero 4.635
Patrickios 3.935
IPC_peptide 4.482
IPC2_peptide 4.622
IPC2.peptide.svr19 4.599
Protein with the highest isoelectric point:
>tr|A0A0Y0IXQ8|A0A0Y0IXQ8_9RHAB Nucleocapsid protein OS=Vesicular stomatitis virus OX=11276 PE=3 SV=1
MM1 pKa = 7.72 SSFKK5 pKa = 10.64 KK6 pKa = 10.4 ILGLSSRR13 pKa = 11.84 SHH15 pKa = 6.23 KK16 pKa = 10.16 KK17 pKa = 9.77 SKK19 pKa = 10.66 KK20 pKa = 9.96 LGLPPPYY27 pKa = 10.09 DD28 pKa = 3.46 EE29 pKa = 4.92 SSPMEE34 pKa = 4.19 IQPSAPLSNDD44 pKa = 2.95 FFGMEE49 pKa = 5.58 DD50 pKa = 3.18 MDD52 pKa = 6.38 LYY54 pKa = 11.49 DD55 pKa = 5.09 KK56 pKa = 11.34 DD57 pKa = 3.49 SLRR60 pKa = 11.84 YY61 pKa = 8.84 EE62 pKa = 4.36 KK63 pKa = 10.58 FRR65 pKa = 11.84 FMLKK69 pKa = 7.95 MTVRR73 pKa = 11.84 SNKK76 pKa = 9.31 PFRR79 pKa = 11.84 SYY81 pKa = 11.86 DD82 pKa = 3.46 DD83 pKa = 3.16 VTAAVSQWDD92 pKa = 3.41 NSYY95 pKa = 10.93 IGMVGKK101 pKa = 10.57 RR102 pKa = 11.84 PFYY105 pKa = 10.59 KK106 pKa = 9.95 IIALIGSSHH115 pKa = 6.42 LQATPAVLADD125 pKa = 4.27 LNQPEE130 pKa = 4.8 YY131 pKa = 10.9 YY132 pKa = 9.36 ATLTGRR138 pKa = 11.84 CFLPHH143 pKa = 7.26 RR144 pKa = 11.84 LGLIPPMFNVSEE156 pKa = 4.38 TFRR159 pKa = 11.84 KK160 pKa = 9.19 PFNIGIYY167 pKa = 10.12 KK168 pKa = 8.76 GTLDD172 pKa = 3.54 FTFTVSDD179 pKa = 3.91 DD180 pKa = 3.65 EE181 pKa = 5.1 SNEE184 pKa = 4.02 KK185 pKa = 10.14 VPHH188 pKa = 5.27 IWEE191 pKa = 4.17 YY192 pKa = 11.07 MNPKK196 pKa = 9.39 YY197 pKa = 10.39 QSQIQKK203 pKa = 10.19 EE204 pKa = 4.28 GLKK207 pKa = 10.63 FGLILSKK214 pKa = 10.55 KK215 pKa = 9.05 ATGTWVLDD223 pKa = 3.31 QLSPFKK229 pKa = 11.04
Molecular weight: 26.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.645
IPC2_protein 8.609
IPC_protein 8.521
Toseland 9.209
ProMoST 9.077
Dawson 9.502
Bjellqvist 9.194
Wikipedia 9.677
Rodwell 9.75
Grimsley 9.575
Solomon 9.56
Lehninger 9.516
Nozaki 9.238
DTASelect 9.18
Thurlkill 9.326
EMBOSS 9.633
Sillero 9.428
Patrickios 4.698
IPC_peptide 9.545
IPC2_peptide 7.702
IPC2.peptide.svr19 7.693
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3551
229
2109
710.2
81.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.168 ± 0.543
1.802 ± 0.223
6.28 ± 0.7
5.829 ± 0.458
4.196 ± 0.315
6.139 ± 0.461
2.534 ± 0.375
7.04 ± 0.619
7.181 ± 0.378
9.434 ± 0.749
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.985 ± 0.289
4.14 ± 0.513
5.182 ± 0.389
3.323 ± 0.252
4.9 ± 0.504
8.448 ± 0.533
5.632 ± 0.46
5.041 ± 0.542
1.971 ± 0.102
3.774 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here