Lactococcus phage 28201

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1ILZ8|A0A1B1ILZ8_9CAUD Uncharacterized protein OS=Lactococcus phage 28201 OX=1871678 GN=DS28201_07 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.03EE3 pKa = 4.4QEE5 pKa = 4.71LNSLLICEE13 pKa = 5.17IEE15 pKa = 4.2NQHH18 pKa = 5.88IDD20 pKa = 3.6YY21 pKa = 11.07RR22 pKa = 11.84LGDD25 pKa = 3.98WNNQVAWVAPLLGLGGYY42 pKa = 7.42EE43 pKa = 3.92KK44 pKa = 10.65NARR47 pKa = 11.84PFDD50 pKa = 3.93HH51 pKa = 6.96AHH53 pKa = 6.01EE54 pKa = 4.77LSHH57 pKa = 7.34ILNHH61 pKa = 6.86DD62 pKa = 3.92DD63 pKa = 4.86YY64 pKa = 11.69RR65 pKa = 11.84GGDD68 pKa = 4.12CDD70 pKa = 3.54TTSPNEE76 pKa = 4.03SRR78 pKa = 11.84AHH80 pKa = 6.33RR81 pKa = 11.84EE82 pKa = 4.04AILLLWDD89 pKa = 3.55MFEE92 pKa = 4.48KK93 pKa = 10.72QGGDD97 pKa = 3.23YY98 pKa = 11.22SHH100 pKa = 7.41FNLFIEE106 pKa = 4.53ITGCPYY112 pKa = 11.09DD113 pKa = 3.48FAYY116 pKa = 10.86SIISKK121 pKa = 9.43EE122 pKa = 3.88FNEE125 pKa = 4.38MYY127 pKa = 10.42EE128 pKa = 4.43AINEE132 pKa = 4.12IFVDD136 pKa = 4.83EE137 pKa = 4.37INIKK141 pKa = 9.7IKK143 pKa = 10.6KK144 pKa = 7.51EE145 pKa = 3.99QIHH148 pKa = 6.3KK149 pKa = 10.25FAVDD153 pKa = 3.52YY154 pKa = 10.83ISYY157 pKa = 10.64FDD159 pKa = 4.47IIEE162 pKa = 4.66SINIYY167 pKa = 10.71NFLEE171 pKa = 4.83AYY173 pKa = 9.68HH174 pKa = 6.66LNHH177 pKa = 6.42SFYY180 pKa = 11.38DD181 pKa = 3.51LAEE184 pKa = 4.44RR185 pKa = 11.84EE186 pKa = 4.21FQEE189 pKa = 4.39LLGVAA194 pKa = 4.42

Molecular weight: 22.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1ILZ5|A0A1B1ILZ5_9CAUD Putative antirepressor OS=Lactococcus phage 28201 OX=1871678 GN=DS28201_06 PE=4 SV=1
MM1 pKa = 7.28GVNRR5 pKa = 11.84KK6 pKa = 8.96CPKK9 pKa = 10.0CGSTHH14 pKa = 5.31VQLSSQKK21 pKa = 11.15SKK23 pKa = 10.38MGWGCLGMIFFGIFYY38 pKa = 10.25FMHH41 pKa = 7.98IFMKK45 pKa = 9.92WAVGLMLFIFVDD57 pKa = 3.91WYY59 pKa = 9.61MAIIKK64 pKa = 9.54AAQNKK69 pKa = 8.78GYY71 pKa = 9.77VWRR74 pKa = 11.84SKK76 pKa = 10.35KK77 pKa = 9.32WFSGKK82 pKa = 9.72KK83 pKa = 6.66QTFFCHH89 pKa = 6.95DD90 pKa = 3.66CGNNFSAA97 pKa = 5.58

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10755

40

1373

210.9

23.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.471 ± 0.377

0.511 ± 0.103

6.378 ± 0.243

7.132 ± 0.631

4.268 ± 0.213

6.592 ± 0.522

1.376 ± 0.189

7.383 ± 0.502

8.294 ± 0.564

7.531 ± 0.463

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.26

6.072 ± 0.388

2.929 ± 0.199

4.073 ± 0.258

3.756 ± 0.338

6.946 ± 0.33

6.927 ± 0.651

5.793 ± 0.222

1.413 ± 0.157

3.654 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski