Amino acid dipepetide frequency for Gordonia phage Hedwig

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.88AlaAla: 17.88 ± 1.447
0.704AlaCys: 0.704 ± 0.28
7.884AlaAsp: 7.884 ± 0.824
7.884AlaGlu: 7.884 ± 0.978
2.886AlaPhe: 2.886 ± 0.662
10.981AlaGly: 10.981 ± 1.096
2.182AlaHis: 2.182 ± 0.424
4.857AlaIle: 4.857 ± 0.716
4.927AlaLys: 4.927 ± 0.873
9.573AlaLeu: 9.573 ± 0.883
4.012AlaMet: 4.012 ± 0.517
2.745AlaAsn: 2.745 ± 0.348
6.687AlaPro: 6.687 ± 0.647
5.561AlaGln: 5.561 ± 0.578
8.166AlaArg: 8.166 ± 0.76
6.969AlaSer: 6.969 ± 0.868
7.884AlaThr: 7.884 ± 1.149
7.954AlaVal: 7.954 ± 0.962
1.337AlaTrp: 1.337 ± 0.305
2.393AlaTyr: 2.393 ± 0.386
0.0AlaXaa: 0.0 ± 0.0
Cys
0.634CysAla: 0.634 ± 0.223
0.282CysCys: 0.282 ± 0.143
0.563CysAsp: 0.563 ± 0.217
0.352CysGlu: 0.352 ± 0.176
0.141CysPhe: 0.141 ± 0.093
1.056CysGly: 1.056 ± 0.35
0.282CysHis: 0.282 ± 0.13
0.0CysIle: 0.0 ± 0.0
0.141CysLys: 0.141 ± 0.106
0.704CysLeu: 0.704 ± 0.228
0.141CysMet: 0.141 ± 0.11
0.352CysAsn: 0.352 ± 0.165
0.704CysPro: 0.704 ± 0.277
0.352CysGln: 0.352 ± 0.172
0.774CysArg: 0.774 ± 0.243
0.211CysSer: 0.211 ± 0.108
0.634CysThr: 0.634 ± 0.197
0.422CysVal: 0.422 ± 0.169
0.352CysTrp: 0.352 ± 0.188
0.141CysTyr: 0.141 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
7.18AspAla: 7.18 ± 0.669
0.634AspCys: 0.634 ± 0.254
5.491AspAsp: 5.491 ± 0.868
5.139AspGlu: 5.139 ± 0.621
1.83AspPhe: 1.83 ± 0.434
6.828AspGly: 6.828 ± 0.771
1.689AspHis: 1.689 ± 0.446
2.745AspIle: 2.745 ± 0.453
1.549AspLys: 1.549 ± 0.29
6.124AspLeu: 6.124 ± 0.886
0.845AspMet: 0.845 ± 0.22
1.619AspAsn: 1.619 ± 0.323
5.139AspPro: 5.139 ± 0.754
2.112AspGln: 2.112 ± 0.406
5.913AspArg: 5.913 ± 0.649
2.534AspSer: 2.534 ± 0.337
3.238AspThr: 3.238 ± 0.544
4.083AspVal: 4.083 ± 0.67
1.126AspTrp: 1.126 ± 0.268
0.774AspTyr: 0.774 ± 0.24
0.0AspXaa: 0.0 ± 0.0
Glu
5.913GluAla: 5.913 ± 0.723
0.493GluCys: 0.493 ± 0.198
3.731GluAsp: 3.731 ± 0.618
3.097GluGlu: 3.097 ± 0.622
2.112GluPhe: 2.112 ± 0.471
4.012GluGly: 4.012 ± 0.507
1.056GluHis: 1.056 ± 0.278
3.52GluIle: 3.52 ± 0.513
1.549GluLys: 1.549 ± 0.324
5.843GluLeu: 5.843 ± 0.843
1.549GluMet: 1.549 ± 0.321
1.549GluAsn: 1.549 ± 0.294
2.675GluPro: 2.675 ± 0.511
2.605GluGln: 2.605 ± 0.444
4.505GluArg: 4.505 ± 0.599
2.816GluSer: 2.816 ± 0.512
3.66GluThr: 3.66 ± 0.461
3.872GluVal: 3.872 ± 0.517
1.126GluTrp: 1.126 ± 0.273
1.76GluTyr: 1.76 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.308PheAla: 3.308 ± 0.463
0.282PheCys: 0.282 ± 0.123
2.745PheAsp: 2.745 ± 0.517
2.253PheGlu: 2.253 ± 0.386
0.634PhePhe: 0.634 ± 0.211
2.605PheGly: 2.605 ± 0.479
0.845PheHis: 0.845 ± 0.254
1.197PheIle: 1.197 ± 0.324
0.422PheLys: 0.422 ± 0.167
1.971PheLeu: 1.971 ± 0.415
0.493PheMet: 0.493 ± 0.174
0.845PheAsn: 0.845 ± 0.224
1.337PhePro: 1.337 ± 0.397
0.704PheGln: 0.704 ± 0.184
1.83PheArg: 1.83 ± 0.341
1.619PheSer: 1.619 ± 0.452
1.971PheThr: 1.971 ± 0.409
1.971PheVal: 1.971 ± 0.365
0.774PheTrp: 0.774 ± 0.21
0.352PheTyr: 0.352 ± 0.132
0.0PheXaa: 0.0 ± 0.0
Gly
8.447GlyAla: 8.447 ± 0.959
0.563GlyCys: 0.563 ± 0.193
6.335GlyAsp: 6.335 ± 0.844
3.942GlyGlu: 3.942 ± 0.441
2.956GlyPhe: 2.956 ± 0.547
8.447GlyGly: 8.447 ± 0.866
2.182GlyHis: 2.182 ± 0.316
4.153GlyIle: 4.153 ± 0.969
2.393GlyLys: 2.393 ± 0.38
6.547GlyLeu: 6.547 ± 0.575
1.549GlyMet: 1.549 ± 0.37
2.253GlyAsn: 2.253 ± 0.377
4.927GlyPro: 4.927 ± 0.525
3.801GlyGln: 3.801 ± 0.372
6.828GlyArg: 6.828 ± 0.664
4.083GlySer: 4.083 ± 0.399
5.772GlyThr: 5.772 ± 0.706
7.039GlyVal: 7.039 ± 0.66
1.83GlyTrp: 1.83 ± 0.287
2.464GlyTyr: 2.464 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
2.323HisAla: 2.323 ± 0.429
0.282HisCys: 0.282 ± 0.131
1.056HisAsp: 1.056 ± 0.224
0.774HisGlu: 0.774 ± 0.232
0.563HisPhe: 0.563 ± 0.169
1.337HisGly: 1.337 ± 0.258
0.493HisHis: 0.493 ± 0.189
1.126HisIle: 1.126 ± 0.23
0.422HisLys: 0.422 ± 0.193
1.901HisLeu: 1.901 ± 0.467
0.211HisMet: 0.211 ± 0.131
0.493HisAsn: 0.493 ± 0.18
1.478HisPro: 1.478 ± 0.371
0.352HisGln: 0.352 ± 0.14
1.689HisArg: 1.689 ± 0.292
1.408HisSer: 1.408 ± 0.372
1.126HisThr: 1.126 ± 0.285
1.197HisVal: 1.197 ± 0.245
0.211HisTrp: 0.211 ± 0.095
0.774HisTyr: 0.774 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
7.11IleAla: 7.11 ± 0.702
0.211IleCys: 0.211 ± 0.128
3.449IleAsp: 3.449 ± 0.536
3.308IleGlu: 3.308 ± 0.61
0.985IlePhe: 0.985 ± 0.285
4.576IleGly: 4.576 ± 0.808
0.915IleHis: 0.915 ± 0.251
1.619IleIle: 1.619 ± 0.364
1.337IleLys: 1.337 ± 0.376
2.745IleLeu: 2.745 ± 0.435
0.915IleMet: 0.915 ± 0.227
1.478IleAsn: 1.478 ± 0.322
1.971IlePro: 1.971 ± 0.369
1.408IleGln: 1.408 ± 0.36
2.393IleArg: 2.393 ± 0.421
1.901IleSer: 1.901 ± 0.507
3.097IleThr: 3.097 ± 0.461
3.449IleVal: 3.449 ± 0.403
1.056IleTrp: 1.056 ± 0.295
1.408IleTyr: 1.408 ± 0.35
0.0IleXaa: 0.0 ± 0.0
Lys
3.59LysAla: 3.59 ± 0.562
0.141LysCys: 0.141 ± 0.101
1.619LysAsp: 1.619 ± 0.334
0.985LysGlu: 0.985 ± 0.305
0.845LysPhe: 0.845 ± 0.268
1.76LysGly: 1.76 ± 0.336
0.634LysHis: 0.634 ± 0.195
1.267LysIle: 1.267 ± 0.231
1.619LysLys: 1.619 ± 0.348
2.605LysLeu: 2.605 ± 0.453
0.704LysMet: 0.704 ± 0.214
0.493LysAsn: 0.493 ± 0.168
1.689LysPro: 1.689 ± 0.298
0.845LysGln: 0.845 ± 0.289
1.689LysArg: 1.689 ± 0.392
2.464LysSer: 2.464 ± 0.461
1.83LysThr: 1.83 ± 0.428
2.605LysVal: 2.605 ± 0.29
0.774LysTrp: 0.774 ± 0.211
0.563LysTyr: 0.563 ± 0.18
0.0LysXaa: 0.0 ± 0.0
Leu
10.77LeuAla: 10.77 ± 0.959
0.563LeuCys: 0.563 ± 0.211
5.068LeuAsp: 5.068 ± 0.844
4.224LeuGlu: 4.224 ± 0.647
3.097LeuPhe: 3.097 ± 0.586
5.913LeuGly: 5.913 ± 1.058
0.915LeuHis: 0.915 ± 0.269
3.379LeuIle: 3.379 ± 0.537
1.549LeuLys: 1.549 ± 0.315
5.561LeuLeu: 5.561 ± 0.734
1.056LeuMet: 1.056 ± 0.259
1.83LeuAsn: 1.83 ± 0.267
5.35LeuPro: 5.35 ± 0.699
2.464LeuGln: 2.464 ± 0.434
6.828LeuArg: 6.828 ± 0.78
4.294LeuSer: 4.294 ± 0.675
5.702LeuThr: 5.702 ± 0.693
7.321LeuVal: 7.321 ± 0.743
1.619LeuTrp: 1.619 ± 0.313
1.267LeuTyr: 1.267 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
2.253MetAla: 2.253 ± 0.422
0.211MetCys: 0.211 ± 0.141
1.056MetAsp: 1.056 ± 0.284
0.704MetGlu: 0.704 ± 0.245
0.704MetPhe: 0.704 ± 0.239
1.478MetGly: 1.478 ± 0.288
0.282MetHis: 0.282 ± 0.171
1.126MetIle: 1.126 ± 0.252
0.704MetLys: 0.704 ± 0.21
2.112MetLeu: 2.112 ± 0.418
0.774MetMet: 0.774 ± 0.258
0.352MetAsn: 0.352 ± 0.155
1.267MetPro: 1.267 ± 0.189
0.352MetGln: 0.352 ± 0.124
1.478MetArg: 1.478 ± 0.314
2.816MetSer: 2.816 ± 0.335
2.112MetThr: 2.112 ± 0.368
0.985MetVal: 0.985 ± 0.266
0.563MetTrp: 0.563 ± 0.206
0.282MetTyr: 0.282 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
2.816AsnAla: 2.816 ± 0.709
0.07AsnCys: 0.07 ± 0.07
1.337AsnAsp: 1.337 ± 0.258
1.337AsnGlu: 1.337 ± 0.227
0.563AsnPhe: 0.563 ± 0.215
2.886AsnGly: 2.886 ± 0.344
0.282AsnHis: 0.282 ± 0.139
1.337AsnIle: 1.337 ± 0.339
0.422AsnLys: 0.422 ± 0.122
1.901AsnLeu: 1.901 ± 0.364
0.282AsnMet: 0.282 ± 0.118
0.985AsnAsn: 0.985 ± 0.359
2.605AsnPro: 2.605 ± 0.46
0.985AsnGln: 0.985 ± 0.277
1.971AsnArg: 1.971 ± 0.405
1.408AsnSer: 1.408 ± 0.388
1.408AsnThr: 1.408 ± 0.311
1.83AsnVal: 1.83 ± 0.511
0.915AsnTrp: 0.915 ± 0.183
0.634AsnTyr: 0.634 ± 0.181
0.0AsnXaa: 0.0 ± 0.0
Pro
8.658ProAla: 8.658 ± 0.944
0.634ProCys: 0.634 ± 0.235
4.857ProAsp: 4.857 ± 0.721
4.224ProGlu: 4.224 ± 0.513
1.478ProPhe: 1.478 ± 0.347
5.631ProGly: 5.631 ± 0.709
0.915ProHis: 0.915 ± 0.204
2.816ProIle: 2.816 ± 0.475
1.901ProLys: 1.901 ± 0.438
3.238ProLeu: 3.238 ± 0.612
0.915ProMet: 0.915 ± 0.256
1.619ProAsn: 1.619 ± 0.364
3.731ProPro: 3.731 ± 0.827
1.76ProGln: 1.76 ± 0.335
3.66ProArg: 3.66 ± 0.58
3.308ProSer: 3.308 ± 0.525
3.942ProThr: 3.942 ± 0.612
4.576ProVal: 4.576 ± 0.45
1.197ProTrp: 1.197 ± 0.229
1.056ProTyr: 1.056 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
4.012GlnAla: 4.012 ± 0.765
0.211GlnCys: 0.211 ± 0.13
1.197GlnAsp: 1.197 ± 0.277
1.901GlnGlu: 1.901 ± 0.333
0.704GlnPhe: 0.704 ± 0.161
2.745GlnGly: 2.745 ± 0.529
0.634GlnHis: 0.634 ± 0.192
2.041GlnIle: 2.041 ± 0.397
0.985GlnLys: 0.985 ± 0.347
2.816GlnLeu: 2.816 ± 0.301
1.197GlnMet: 1.197 ± 0.262
0.704GlnAsn: 0.704 ± 0.226
2.464GlnPro: 2.464 ± 0.403
1.408GlnGln: 1.408 ± 0.351
3.238GlnArg: 3.238 ± 0.405
2.745GlnSer: 2.745 ± 0.473
2.041GlnThr: 2.041 ± 0.324
3.097GlnVal: 3.097 ± 0.473
0.845GlnTrp: 0.845 ± 0.294
0.774GlnTyr: 0.774 ± 0.18
0.0GlnXaa: 0.0 ± 0.0
Arg
8.518ArgAla: 8.518 ± 0.769
0.845ArgCys: 0.845 ± 0.307
4.927ArgAsp: 4.927 ± 0.49
3.731ArgGlu: 3.731 ± 0.49
1.971ArgPhe: 1.971 ± 0.443
5.209ArgGly: 5.209 ± 0.768
1.901ArgHis: 1.901 ± 0.444
3.59ArgIle: 3.59 ± 0.422
2.816ArgLys: 2.816 ± 0.41
6.054ArgLeu: 6.054 ± 0.592
1.971ArgMet: 1.971 ± 0.386
2.041ArgAsn: 2.041 ± 0.405
3.872ArgPro: 3.872 ± 0.828
2.816ArgGln: 2.816 ± 0.44
7.743ArgArg: 7.743 ± 1.288
4.435ArgSer: 4.435 ± 0.661
4.927ArgThr: 4.927 ± 0.545
4.716ArgVal: 4.716 ± 0.528
1.197ArgTrp: 1.197 ± 0.31
1.478ArgTyr: 1.478 ± 0.353
0.0ArgXaa: 0.0 ± 0.0
Ser
8.377SerAla: 8.377 ± 0.782
0.352SerCys: 0.352 ± 0.177
3.168SerAsp: 3.168 ± 0.476
3.942SerGlu: 3.942 ± 0.459
1.76SerPhe: 1.76 ± 0.345
6.124SerGly: 6.124 ± 0.674
1.197SerHis: 1.197 ± 0.283
2.956SerIle: 2.956 ± 0.507
1.056SerLys: 1.056 ± 0.304
3.801SerLeu: 3.801 ± 0.58
1.619SerMet: 1.619 ± 0.357
1.76SerAsn: 1.76 ± 0.3
3.168SerPro: 3.168 ± 0.412
2.605SerGln: 2.605 ± 0.425
3.801SerArg: 3.801 ± 0.566
3.379SerSer: 3.379 ± 0.486
3.238SerThr: 3.238 ± 0.507
3.379SerVal: 3.379 ± 0.381
1.056SerTrp: 1.056 ± 0.289
1.197SerTyr: 1.197 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
7.321ThrAla: 7.321 ± 0.908
0.422ThrCys: 0.422 ± 0.203
4.787ThrAsp: 4.787 ± 0.544
3.238ThrGlu: 3.238 ± 0.473
1.408ThrPhe: 1.408 ± 0.274
6.617ThrGly: 6.617 ± 0.7
0.845ThrHis: 0.845 ± 0.207
2.956ThrIle: 2.956 ± 0.432
1.408ThrLys: 1.408 ± 0.287
5.42ThrLeu: 5.42 ± 0.721
1.197ThrMet: 1.197 ± 0.287
1.619ThrAsn: 1.619 ± 0.441
5.139ThrPro: 5.139 ± 0.618
1.971ThrGln: 1.971 ± 0.369
3.027ThrArg: 3.027 ± 0.459
3.801ThrSer: 3.801 ± 0.496
5.983ThrThr: 5.983 ± 0.705
7.25ThrVal: 7.25 ± 0.754
1.619ThrTrp: 1.619 ± 0.346
0.845ThrTyr: 0.845 ± 0.301
0.0ThrXaa: 0.0 ± 0.0
Val
9.925ValAla: 9.925 ± 0.984
0.563ValCys: 0.563 ± 0.187
4.505ValAsp: 4.505 ± 0.682
4.153ValGlu: 4.153 ± 0.717
1.83ValPhe: 1.83 ± 0.389
5.35ValGly: 5.35 ± 0.573
1.408ValHis: 1.408 ± 0.304
3.168ValIle: 3.168 ± 0.526
2.253ValLys: 2.253 ± 0.406
5.772ValLeu: 5.772 ± 0.629
1.337ValMet: 1.337 ± 0.364
1.76ValAsn: 1.76 ± 0.324
4.224ValPro: 4.224 ± 0.55
2.253ValGln: 2.253 ± 0.579
6.406ValArg: 6.406 ± 0.801
5.139ValSer: 5.139 ± 0.57
5.35ValThr: 5.35 ± 0.556
5.139ValVal: 5.139 ± 0.683
1.83ValTrp: 1.83 ± 0.376
1.337ValTyr: 1.337 ± 0.301
0.0ValXaa: 0.0 ± 0.0
Trp
1.549TrpAla: 1.549 ± 0.282
0.704TrpCys: 0.704 ± 0.234
1.83TrpAsp: 1.83 ± 0.272
0.845TrpGlu: 0.845 ± 0.341
0.915TrpPhe: 0.915 ± 0.205
1.267TrpGly: 1.267 ± 0.255
0.352TrpHis: 0.352 ± 0.133
0.915TrpIle: 0.915 ± 0.284
0.704TrpLys: 0.704 ± 0.217
2.323TrpLeu: 2.323 ± 0.417
0.563TrpMet: 0.563 ± 0.148
0.774TrpAsn: 0.774 ± 0.338
0.634TrpPro: 0.634 ± 0.248
0.774TrpGln: 0.774 ± 0.223
1.267TrpArg: 1.267 ± 0.23
1.76TrpSer: 1.76 ± 0.393
1.267TrpThr: 1.267 ± 0.358
1.267TrpVal: 1.267 ± 0.29
0.352TrpTrp: 0.352 ± 0.161
0.352TrpTyr: 0.352 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.886TyrAla: 2.886 ± 0.368
0.07TyrCys: 0.07 ± 0.069
0.985TyrAsp: 0.985 ± 0.294
1.408TyrGlu: 1.408 ± 0.314
0.774TyrPhe: 0.774 ± 0.209
1.689TyrGly: 1.689 ± 0.393
0.211TyrHis: 0.211 ± 0.12
0.563TyrIle: 0.563 ± 0.208
0.563TyrLys: 0.563 ± 0.207
1.83TyrLeu: 1.83 ± 0.37
0.211TyrMet: 0.211 ± 0.098
0.704TyrAsn: 0.704 ± 0.166
1.056TyrPro: 1.056 ± 0.276
0.634TyrGln: 0.634 ± 0.245
1.549TyrArg: 1.549 ± 0.352
0.845TyrSer: 0.845 ± 0.244
1.689TyrThr: 1.689 ± 0.363
1.549TyrVal: 1.549 ± 0.358
0.634TyrTrp: 0.634 ± 0.258
0.563TyrTyr: 0.563 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (14207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski