Tobacco leaf curl betasatellite-[Pakistan:Multan:Pedilanthus:2004]
Average proteome isoelectric point is 4.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7ZDP2|B7ZDP2_9VIRU C1 protein OS=Tobacco leaf curl betasatellite-[Pakistan:Multan:Pedilanthus:2004] OX=587314 GN=C1 PE=4 SV=1
MM1 pKa = 7.53 TYY3 pKa = 10.72 AHH5 pKa = 6.54 SHH7 pKa = 4.0 NTMTIKK13 pKa = 10.72 YY14 pKa = 8.52 KK15 pKa = 9.19 NQKK18 pKa = 9.54 GMEE21 pKa = 4.43 FIIDD25 pKa = 3.52 VRR27 pKa = 11.84 LKK29 pKa = 9.83 EE30 pKa = 4.38 DD31 pKa = 3.2 NSILVQIQLFSTRR44 pKa = 11.84 SPALATRR51 pKa = 11.84 KK52 pKa = 9.8 FMIPYY57 pKa = 7.78 EE58 pKa = 4.28 HH59 pKa = 6.94 NGIIPPFNFNTLEE72 pKa = 3.96 QGIHH76 pKa = 5.74 NMLALMYY83 pKa = 9.42 KK84 pKa = 10.2 DD85 pKa = 4.74 SNIGDD90 pKa = 4.59 FKK92 pKa = 11.22 QEE94 pKa = 4.37 DD95 pKa = 4.06 MVEE98 pKa = 4.39 CIDD101 pKa = 3.66 MLMMEE106 pKa = 5.27 EE107 pKa = 4.94 APVIDD112 pKa = 4.34 INVMDD117 pKa = 5.27 AYY119 pKa = 10.57 DD120 pKa = 4.0 VYY122 pKa = 10.56 THH124 pKa = 6.81 SSVV127 pKa = 3.05
Molecular weight: 14.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.891
IPC2_protein 4.991
IPC_protein 4.889
Toseland 4.863
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.041
Wikipedia 4.813
Rodwell 4.825
Grimsley 4.8
Solomon 4.914
Lehninger 4.876
Nozaki 5.041
DTASelect 5.219
Thurlkill 4.876
EMBOSS 4.863
Sillero 5.105
Patrickios 3.656
IPC_peptide 4.914
IPC2_peptide 5.105
IPC2.peptide.svr19 5.019
Protein with the highest isoelectric point:
>tr|B7ZDP2|B7ZDP2_9VIRU C1 protein OS=Tobacco leaf curl betasatellite-[Pakistan:Multan:Pedilanthus:2004] OX=587314 GN=C1 PE=4 SV=1
MM1 pKa = 7.53 TYY3 pKa = 10.72 AHH5 pKa = 6.54 SHH7 pKa = 4.0 NTMTIKK13 pKa = 10.72 YY14 pKa = 8.52 KK15 pKa = 9.19 NQKK18 pKa = 9.54 GMEE21 pKa = 4.43 FIIDD25 pKa = 3.52 VRR27 pKa = 11.84 LKK29 pKa = 9.83 EE30 pKa = 4.38 DD31 pKa = 3.2 NSILVQIQLFSTRR44 pKa = 11.84 SPALATRR51 pKa = 11.84 KK52 pKa = 9.8 FMIPYY57 pKa = 7.78 EE58 pKa = 4.28 HH59 pKa = 6.94 NGIIPPFNFNTLEE72 pKa = 3.96 QGIHH76 pKa = 5.74 NMLALMYY83 pKa = 9.42 KK84 pKa = 10.2 DD85 pKa = 4.74 SNIGDD90 pKa = 4.59 FKK92 pKa = 11.22 QEE94 pKa = 4.37 DD95 pKa = 4.06 MVEE98 pKa = 4.39 CIDD101 pKa = 3.66 MLMMEE106 pKa = 5.27 EE107 pKa = 4.94 APVIDD112 pKa = 4.34 INVMDD117 pKa = 5.27 AYY119 pKa = 10.57 DD120 pKa = 4.0 VYY122 pKa = 10.56 THH124 pKa = 6.81 SSVV127 pKa = 3.05
Molecular weight: 14.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.891
IPC2_protein 4.991
IPC_protein 4.889
Toseland 4.863
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.041
Wikipedia 4.813
Rodwell 4.825
Grimsley 4.8
Solomon 4.914
Lehninger 4.876
Nozaki 5.041
DTASelect 5.219
Thurlkill 4.876
EMBOSS 4.863
Sillero 5.105
Patrickios 3.656
IPC_peptide 4.914
IPC2_peptide 5.105
IPC2.peptide.svr19 5.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
127
127
127
127.0
14.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.724 ± 0.0
0.787 ± 0.0
7.087 ± 0.0
6.299 ± 0.0
4.724 ± 0.0
3.15 ± 0.0
3.937 ± 0.0
10.236 ± 0.0
5.512 ± 0.0
6.299 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
8.661 ± 0.0
7.087 ± 0.0
3.937 ± 0.0
3.937 ± 0.0
2.362 ± 0.0
5.512 ± 0.0
5.512 ± 0.0
5.512 ± 0.0
0.0 ± 0.0
4.724 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here