Amino acid dipepetide frequency for Myotis lucifugus (Little brown bat)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.74AlaAla: 6.74 ± 0.048	1.458AlaCys: 1.458 ± 0.015	2.935AlaAsp: 2.935 ± 0.019	4.702AlaGlu: 4.702 ± 0.026	2.63AlaPhe: 2.63 ± 0.019	4.845AlaGly: 4.845 ± 0.032	1.612AlaHis: 1.612 ± 0.013	2.797AlaIle: 2.797 ± 0.018	3.401AlaLys: 3.401 ± 0.025	7.274AlaLeu: 7.274 ± 0.039	1.518AlaMet: 1.518 ± 0.014	2.004AlaAsn: 2.004 ± 0.014	4.345AlaPro: 4.345 ± 0.035	3.329AlaGln: 3.329 ± 0.027	3.707AlaArg: 3.707 ± 0.026	5.716AlaSer: 5.716 ± 0.03	3.503AlaThr: 3.503 ± 0.02	4.705AlaVal: 4.705 ± 0.026	0.827AlaTrp: 0.827 ± 0.01	1.539AlaTyr: 1.539 ± 0.015	0.002AlaXaa: 0.002 ± 0.001
Cys	1.353CysAla: 1.353 ± 0.014	0.718CysCys: 0.718 ± 0.02	1.018CysAsp: 1.018 ± 0.015	1.266CysGlu: 1.266 ± 0.015	0.883CysPhe: 0.883 ± 0.01	1.667CysGly: 1.667 ± 0.019	0.69CysHis: 0.69 ± 0.01	0.964CysIle: 0.964 ± 0.011	1.128CysLys: 1.128 ± 0.012	2.233CysLeu: 2.233 ± 0.018	0.416CysMet: 0.416 ± 0.007	0.795CysAsn: 0.795 ± 0.011	1.468CysPro: 1.468 ± 0.019	1.103CysGln: 1.103 ± 0.016	1.356CysArg: 1.356 ± 0.014	2.053CysSer: 2.053 ± 0.018	1.138CysThr: 1.138 ± 0.014	1.333CysVal: 1.333 ± 0.012	0.316CysTrp: 0.316 ± 0.006	0.617CysTyr: 0.617 ± 0.01	0.001CysXaa: 0.001 ± 0.0
Asp	2.827AspAla: 2.827 ± 0.02	1.05AspCys: 1.05 ± 0.013	2.527AspAsp: 2.527 ± 0.021	3.319AspGlu: 3.319 ± 0.022	2.109AspPhe: 2.109 ± 0.018	3.256AspGly: 3.256 ± 0.025	1.159AspHis: 1.159 ± 0.011	2.6AspIle: 2.6 ± 0.019	2.485AspLys: 2.485 ± 0.018	4.989AspLeu: 4.989 ± 0.024	1.115AspMet: 1.115 ± 0.011	1.645AspAsn: 1.645 ± 0.017	2.967AspPro: 2.967 ± 0.022	1.843AspGln: 1.843 ± 0.014	2.462AspArg: 2.462 ± 0.02	4.106AspSer: 4.106 ± 0.026	2.498AspThr: 2.498 ± 0.016	3.011AspVal: 3.011 ± 0.027	0.633AspTrp: 0.633 ± 0.01	1.453AspTyr: 1.453 ± 0.015	0.001AspXaa: 0.001 ± 0.0
Glu	5.246GluAla: 5.246 ± 0.034	1.304GluCys: 1.304 ± 0.019	4.389GluAsp: 4.389 ± 0.027	7.81GluGlu: 7.81 ± 0.05	2.047GluPhe: 2.047 ± 0.013	4.238GluGly: 4.238 ± 0.026	1.5GluHis: 1.5 ± 0.012	3.08GluIle: 3.08 ± 0.024	5.173GluLys: 5.173 ± 0.036	6.385GluLeu: 6.385 ± 0.038	1.638GluMet: 1.638 ± 0.015	3.054GluAsn: 3.054 ± 0.021	3.301GluPro: 3.301 ± 0.029	3.114GluGln: 3.114 ± 0.024	3.877GluArg: 3.877 ± 0.03	4.274GluSer: 4.274 ± 0.028	3.266GluThr: 3.266 ± 0.019	4.161GluVal: 4.161 ± 0.026	0.734GluTrp: 0.734 ± 0.009	1.549GluTyr: 1.549 ± 0.017	0.002GluXaa: 0.002 ± 0.0
Phe	1.983PheAla: 1.983 ± 0.017	0.995PheCys: 0.995 ± 0.011	1.659PheAsp: 1.659 ± 0.014	1.98PheGlu: 1.98 ± 0.016	1.702PhePhe: 1.702 ± 0.016	2.235PheGly: 2.235 ± 0.018	1.066PheHis: 1.066 ± 0.011	1.872PheIle: 1.872 ± 0.017	1.793PheLys: 1.793 ± 0.015	4.313PheLeu: 4.313 ± 0.027	0.762PheMet: 0.762 ± 0.008	1.325PheAsn: 1.325 ± 0.013	2.098PhePro: 2.098 ± 0.017	1.845PheGln: 1.845 ± 0.014	2.008PheArg: 2.008 ± 0.018	3.5PheSer: 3.5 ± 0.021	2.034PheThr: 2.034 ± 0.017	2.177PheVal: 2.177 ± 0.017	0.508PheTrp: 0.508 ± 0.007	1.25PheTyr: 1.25 ± 0.012	0.001PheXaa: 0.001 ± 0.0
Gly	4.643GlyAla: 4.643 ± 0.035	1.328GlyCys: 1.328 ± 0.014	3.124GlyAsp: 3.124 ± 0.021	4.077GlyGlu: 4.077 ± 0.029	2.47GlyPhe: 2.47 ± 0.02	5.127GlyGly: 5.127 ± 0.05	1.757GlyHis: 1.757 ± 0.017	2.716GlyIle: 2.716 ± 0.023	3.739GlyLys: 3.739 ± 0.027	6.092GlyLeu: 6.092 ± 0.035	1.274GlyMet: 1.274 ± 0.012	2.36GlyAsn: 2.36 ± 0.018	4.481GlyPro: 4.481 ± 0.055	2.806GlyGln: 2.806 ± 0.022	3.847GlyArg: 3.847 ± 0.025	5.846GlySer: 5.846 ± 0.031	3.614GlyThr: 3.614 ± 0.023	3.638GlyVal: 3.638 ± 0.023	0.825GlyTrp: 0.825 ± 0.011	1.733GlyTyr: 1.733 ± 0.018	0.002GlyXaa: 0.002 ± 0.0
His	1.334HisAla: 1.334 ± 0.014	0.741HisCys: 0.741 ± 0.01	0.866HisAsp: 0.866 ± 0.01	1.26HisGlu: 1.26 ± 0.012	1.157HisPhe: 1.157 ± 0.012	1.568HisGly: 1.568 ± 0.014	0.876HisHis: 0.876 ± 0.012	1.263HisIle: 1.263 ± 0.012	1.234HisLys: 1.234 ± 0.013	3.003HisLeu: 3.003 ± 0.02	0.584HisMet: 0.584 ± 0.009	0.862HisAsn: 0.862 ± 0.01	1.725HisPro: 1.725 ± 0.015	1.305HisGln: 1.305 ± 0.015	1.658HisArg: 1.658 ± 0.014	2.276HisSer: 2.276 ± 0.018	1.449HisThr: 1.449 ± 0.014	1.524HisVal: 1.524 ± 0.013	0.368HisTrp: 0.368 ± 0.006	0.824HisTyr: 0.824 ± 0.009	0.001HisXaa: 0.001 ± 0.0
Ile	2.549IleAla: 2.549 ± 0.019	1.101IleCys: 1.101 ± 0.012	1.968IleAsp: 1.968 ± 0.017	2.497IleGlu: 2.497 ± 0.02	1.93IlePhe: 1.93 ± 0.019	2.182IleGly: 2.182 ± 0.02	1.315IleHis: 1.315 ± 0.012	2.362IleIle: 2.362 ± 0.024	2.56IleLys: 2.56 ± 0.022	4.643IleLeu: 4.643 ± 0.029	0.983IleMet: 0.983 ± 0.009	1.78IleAsn: 1.78 ± 0.017	2.589IlePro: 2.589 ± 0.017	2.265IleGln: 2.265 ± 0.015	2.368IleArg: 2.368 ± 0.018	3.689IleSer: 3.689 ± 0.023	2.525IleThr: 2.525 ± 0.021	2.476IleVal: 2.476 ± 0.021	0.549IleTrp: 0.549 ± 0.009	1.42IleTyr: 1.42 ± 0.011	0.001IleXaa: 0.001 ± 0.0
Lys	3.998LysAla: 3.998 ± 0.028	1.09LysCys: 1.09 ± 0.013	3.095LysAsp: 3.095 ± 0.025	4.981LysGlu: 4.981 ± 0.037	1.73LysPhe: 1.73 ± 0.016	3.312LysGly: 3.312 ± 0.032	1.392LysHis: 1.392 ± 0.013	2.735LysIle: 2.735 ± 0.019	4.705LysLys: 4.705 ± 0.04	5.088LysLeu: 5.088 ± 0.026	1.439LysMet: 1.439 ± 0.015	2.373LysAsn: 2.373 ± 0.019	3.043LysPro: 3.043 ± 0.028	2.558LysGln: 2.558 ± 0.022	3.273LysArg: 3.273 ± 0.021	3.801LysSer: 3.801 ± 0.029	3.046LysThr: 3.046 ± 0.022	3.455LysVal: 3.455 ± 0.032	0.597LysTrp: 0.597 ± 0.008	1.58LysTyr: 1.58 ± 0.015	0.001LysXaa: 0.001 ± 0.0
Leu	6.911LeuAla: 6.911 ± 0.037	2.29LeuCys: 2.29 ± 0.018	4.749LeuAsp: 4.749 ± 0.026	7.118LeuGlu: 7.118 ± 0.04	3.539LeuPhe: 3.539 ± 0.024	6.068LeuGly: 6.068 ± 0.033	2.752LeuHis: 2.752 ± 0.016	3.885LeuIle: 3.885 ± 0.028	5.793LeuLys: 5.793 ± 0.035	11.052LeuLeu: 11.052 ± 0.065	1.957LeuMet: 1.957 ± 0.015	3.477LeuAsn: 3.477 ± 0.023	6.151LeuPro: 6.151 ± 0.035	5.839LeuGln: 5.839 ± 0.038	6.071LeuArg: 6.071 ± 0.035	8.205LeuSer: 8.205 ± 0.033	5.15LeuThr: 5.15 ± 0.023	5.726LeuVal: 5.726 ± 0.033	1.195LeuTrp: 1.195 ± 0.013	2.582LeuTyr: 2.582 ± 0.018	0.003LeuXaa: 0.003 ± 0.0
Met	1.901MetAla: 1.901 ± 0.016	0.405MetCys: 0.405 ± 0.007	1.22MetAsp: 1.22 ± 0.012	1.806MetGlu: 1.806 ± 0.014	0.729MetPhe: 0.729 ± 0.009	1.278MetGly: 1.278 ± 0.013	0.451MetHis: 0.451 ± 0.007	0.816MetIle: 0.816 ± 0.009	1.419MetLys: 1.419 ± 0.014	1.991MetLeu: 1.991 ± 0.018	0.535MetMet: 0.535 ± 0.009	0.88MetAsn: 0.88 ± 0.009	1.074MetPro: 1.074 ± 0.016	0.902MetGln: 0.902 ± 0.01	1.0MetArg: 1.0 ± 0.01	1.545MetSer: 1.545 ± 0.015	1.089MetThr: 1.089 ± 0.012	1.361MetVal: 1.361 ± 0.014	0.24MetTrp: 0.24 ± 0.005	0.617MetTyr: 0.617 ± 0.01	0.001MetXaa: 0.001 ± 0.0
Asn	2.037AsnAla: 2.037 ± 0.015	0.853AsnCys: 0.853 ± 0.01	1.48AsnAsp: 1.48 ± 0.014	2.157AsnGlu: 2.157 ± 0.019	1.487AsnPhe: 1.487 ± 0.013	2.357AsnGly: 2.357 ± 0.018	0.926AsnHis: 0.926 ± 0.01	2.081AsnIle: 2.081 ± 0.017	2.181AsnLys: 2.181 ± 0.017	3.712AsnLeu: 3.712 ± 0.022	0.884AsnMet: 0.884 ± 0.009	1.504AsnAsn: 1.504 ± 0.016	2.189AsnPro: 2.189 ± 0.017	1.632AsnGln: 1.632 ± 0.015	1.815AsnArg: 1.815 ± 0.013	2.998AsnSer: 2.998 ± 0.022	1.94AsnThr: 1.94 ± 0.014	2.188AsnVal: 2.188 ± 0.015	0.455AsnTrp: 0.455 ± 0.007	1.123AsnTyr: 1.123 ± 0.012	0.001AsnXaa: 0.001 ± 0.0
Pro	4.923ProAla: 4.923 ± 0.034	1.207ProCys: 1.207 ± 0.017	2.763ProAsp: 2.763 ± 0.022	4.487ProGlu: 4.487 ± 0.031	1.949ProPhe: 1.949 ± 0.015	5.589ProGly: 5.589 ± 0.076	1.467ProHis: 1.467 ± 0.014	1.88ProIle: 1.88 ± 0.018	2.851ProLys: 2.851 ± 0.033	5.401ProLeu: 5.401 ± 0.03	1.082ProMet: 1.082 ± 0.012	1.808ProAsn: 1.808 ± 0.013	6.111ProPro: 6.111 ± 0.058	2.88ProGln: 2.88 ± 0.024	3.538ProArg: 3.538 ± 0.027	5.731ProSer: 5.731 ± 0.035	3.089ProThr: 3.089 ± 0.024	3.858ProVal: 3.858 ± 0.026	0.738ProTrp: 0.738 ± 0.01	1.423ProTyr: 1.423 ± 0.015	0.002ProXaa: 0.002 ± 0.0
Gln	3.598GlnAla: 3.598 ± 0.025	0.95GlnCys: 0.95 ± 0.014	2.35GlnAsp: 2.35 ± 0.019	3.93GlnGlu: 3.93 ± 0.03	1.364GlnPhe: 1.364 ± 0.012	2.957GlnGly: 2.957 ± 0.023	1.294GlnHis: 1.294 ± 0.014	1.951GlnIle: 1.951 ± 0.014	2.912GlnLys: 2.912 ± 0.024	4.789GlnLeu: 4.789 ± 0.034	1.107GlnMet: 1.107 ± 0.012	1.84GlnAsn: 1.84 ± 0.019	2.827GlnPro: 2.827 ± 0.028	3.01GlnGln: 3.01 ± 0.039	2.97GlnArg: 2.97 ± 0.023	3.053GlnSer: 3.053 ± 0.024	2.275GlnThr: 2.275 ± 0.017	2.923GlnVal: 2.923 ± 0.019	0.552GlnTrp: 0.552 ± 0.008	1.131GlnTyr: 1.131 ± 0.012	0.001GlnXaa: 0.001 ± 0.0
Arg	3.96ArgAla: 3.96 ± 0.027	1.277ArgCys: 1.277 ± 0.017	2.793ArgAsp: 2.793 ± 0.021	3.992ArgGlu: 3.992 ± 0.029	1.925ArgPhe: 1.925 ± 0.016	3.754ArgGly: 3.754 ± 0.031	1.552ArgHis: 1.552 ± 0.015	2.341ArgIle: 2.341 ± 0.018	3.716ArgLys: 3.716 ± 0.023	5.449ArgLeu: 5.449 ± 0.029	1.143ArgMet: 1.143 ± 0.012	2.117ArgAsn: 2.117 ± 0.014	3.331ArgPro: 3.331 ± 0.025	2.624ArgGln: 2.624 ± 0.02	4.356ArgArg: 4.356 ± 0.04	4.186ArgSer: 4.186 ± 0.035	2.784ArgThr: 2.784 ± 0.02	3.192ArgVal: 3.192 ± 0.022	0.731ArgTrp: 0.731 ± 0.009	1.494ArgTyr: 1.494 ± 0.013	0.002ArgXaa: 0.002 ± 0.0
Ser	5.279SerAla: 5.279 ± 0.027	1.922SerCys: 1.922 ± 0.021	3.828SerAsp: 3.828 ± 0.027	5.048SerGlu: 5.048 ± 0.031	3.085SerPhe: 3.085 ± 0.019	5.627SerGly: 5.627 ± 0.029	2.138SerHis: 2.138 ± 0.019	3.174SerIle: 3.174 ± 0.021	3.995SerLys: 3.995 ± 0.027	8.39SerLeu: 8.39 ± 0.037	1.538SerMet: 1.538 ± 0.012	2.635SerAsn: 2.635 ± 0.019	5.998SerPro: 5.998 ± 0.041	3.849SerGln: 3.849 ± 0.027	4.516SerArg: 4.516 ± 0.035	8.974SerSer: 8.974 ± 0.066	4.297SerThr: 4.297 ± 0.026	4.907SerVal: 4.907 ± 0.027	1.114SerTrp: 1.114 ± 0.013	2.098SerTyr: 2.098 ± 0.017	0.002SerXaa: 0.002 ± 0.0
Thr	3.696ThrAla: 3.696 ± 0.023	1.373ThrCys: 1.373 ± 0.017	2.424ThrAsp: 2.424 ± 0.017	3.449ThrGlu: 3.449 ± 0.021	2.149ThrPhe: 2.149 ± 0.016	3.486ThrGly: 3.486 ± 0.027	1.282ThrHis: 1.282 ± 0.014	2.388ThrIle: 2.388 ± 0.023	2.62ThrLys: 2.62 ± 0.02	5.328ThrLeu: 5.328 ± 0.027	1.074ThrMet: 1.074 ± 0.01	1.702ThrAsn: 1.702 ± 0.016	3.494ThrPro: 3.494 ± 0.027	2.279ThrGln: 2.279 ± 0.018	2.44ThrArg: 2.44 ± 0.018	4.486ThrSer: 4.486 ± 0.027	2.854ThrThr: 2.854 ± 0.027	3.867ThrVal: 3.867 ± 0.028	0.709ThrTrp: 0.709 ± 0.009	1.456ThrTyr: 1.456 ± 0.015	0.002ThrXaa: 0.002 ± 0.0
Val	4.299ValAla: 4.299 ± 0.024	1.478ValCys: 1.478 ± 0.012	2.91ValAsp: 2.91 ± 0.021	3.803ValGlu: 3.803 ± 0.027	2.455ValPhe: 2.455 ± 0.018	3.463ValGly: 3.463 ± 0.023	1.581ValHis: 1.581 ± 0.014	2.913ValIle: 2.913 ± 0.023	3.348ValLys: 3.348 ± 0.027	6.393ValLeu: 6.393 ± 0.034	1.308ValMet: 1.308 ± 0.013	2.196ValAsn: 2.196 ± 0.019	3.737ValPro: 3.737 ± 0.03	2.797ValGln: 2.797 ± 0.017	3.076ValArg: 3.076 ± 0.022	4.881ValSer: 4.881 ± 0.026	3.833ValThr: 3.833 ± 0.035	3.996ValVal: 3.996 ± 0.03	0.712ValTrp: 0.712 ± 0.009	1.659ValTyr: 1.659 ± 0.015	0.001ValXaa: 0.001 ± 0.0
Trp	0.867TrpAla: 0.867 ± 0.009	0.255TrpCys: 0.255 ± 0.005	0.668TrpAsp: 0.668 ± 0.011	0.836TrpGlu: 0.836 ± 0.011	0.455TrpPhe: 0.455 ± 0.007	0.784TrpGly: 0.784 ± 0.011	0.319TrpHis: 0.319 ± 0.006	0.565TrpIle: 0.565 ± 0.007	0.803TrpLys: 0.803 ± 0.01	1.245TrpLeu: 1.245 ± 0.013	0.313TrpMet: 0.313 ± 0.005	0.535TrpAsn: 0.535 ± 0.009	0.534TrpPro: 0.534 ± 0.007	0.562TrpGln: 0.562 ± 0.008	0.755TrpArg: 0.755 ± 0.009	0.89TrpSer: 0.89 ± 0.01	0.685TrpThr: 0.685 ± 0.009	0.759TrpVal: 0.759 ± 0.009	0.198TrpTrp: 0.198 ± 0.005	0.353TrpTyr: 0.353 ± 0.006	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.443TyrAla: 1.443 ± 0.013	0.719TyrCys: 0.719 ± 0.01	1.287TyrAsp: 1.287 ± 0.014	1.624TyrGlu: 1.624 ± 0.017	1.309TyrPhe: 1.309 ± 0.013	1.703TyrGly: 1.703 ± 0.016	0.751TyrHis: 0.751 ± 0.01	1.368TyrIle: 1.368 ± 0.012	1.435TyrLys: 1.435 ± 0.018	2.739TyrLeu: 2.739 ± 0.019	0.589TyrMet: 0.589 ± 0.009	1.107TyrAsn: 1.107 ± 0.012	1.31TyrPro: 1.31 ± 0.014	1.241TyrGln: 1.241 ± 0.012	1.624TyrArg: 1.624 ± 0.015	2.202TyrSer: 2.202 ± 0.021	1.489TyrThr: 1.489 ± 0.017	1.584TyrVal: 1.584 ± 0.015	0.377TyrTrp: 0.377 ± 0.008	0.998TyrTyr: 0.998 ± 0.012	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.002XaaLeu: 0.002 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.002XaaGln: 0.002 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.003XaaSer: 0.003 ± 0.001	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.007XaaXaa: 0.007 ± 0.002

Statistics based on 20651 proteins (10579164 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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