Clostridium sp. CAG:448

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6SU70|R6SU70_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:448 OX=1262808 GN=BN660_01010 PE=4 SV=1
MM1 pKa = 7.01TKK3 pKa = 9.8IVFFRR8 pKa = 11.84SDD10 pKa = 2.56GFYY13 pKa = 10.89YY14 pKa = 10.53GFEE17 pKa = 4.38EE18 pKa = 4.83QGHH21 pKa = 5.23TGYY24 pKa = 11.1GEE26 pKa = 4.12EE27 pKa = 5.06GDD29 pKa = 5.26DD30 pKa = 3.89VLCAALSAMTMLIINTIEE48 pKa = 3.86VAFDD52 pKa = 3.4SDD54 pKa = 3.03VDD56 pKa = 3.82YY57 pKa = 11.39EE58 pKa = 4.25IDD60 pKa = 4.25EE61 pKa = 4.41KK62 pKa = 9.8TTDD65 pKa = 2.97IRR67 pKa = 11.84VKK69 pKa = 10.78AKK71 pKa = 10.38AALPEE76 pKa = 4.2YY77 pKa = 10.59EE78 pKa = 4.68DD79 pKa = 6.07DD80 pKa = 3.64EE81 pKa = 4.8LKK83 pKa = 10.56RR84 pKa = 11.84YY85 pKa = 8.88AVSGMFLGYY94 pKa = 10.2YY95 pKa = 9.43KK96 pKa = 10.27QLEE99 pKa = 4.39DD100 pKa = 4.01MLEE103 pKa = 3.87EE104 pKa = 4.45YY105 pKa = 11.08YY106 pKa = 10.74DD107 pKa = 4.02YY108 pKa = 11.52LDD110 pKa = 4.44LAIEE114 pKa = 4.29DD115 pKa = 3.62RR116 pKa = 11.84PYY118 pKa = 11.55

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6SKR2|R6SKR2_9CLOT Riboflavin biosynthesis protein OS=Clostridium sp. CAG:448 OX=1262808 GN=BN660_00755 PE=3 SV=1
MM1 pKa = 7.37NKK3 pKa = 9.74DD4 pKa = 3.33HH5 pKa = 7.23NINAGRR11 pKa = 11.84QSFLRR16 pKa = 11.84RR17 pKa = 11.84DD18 pKa = 3.56LSSLLTRR25 pKa = 11.84GGFMNKK31 pKa = 8.27KK32 pKa = 8.74TDD34 pKa = 3.65FGDD37 pKa = 3.76NLWQYY42 pKa = 11.43RR43 pKa = 11.84SMRR46 pKa = 11.84MKK48 pKa = 10.64KK49 pKa = 9.96RR50 pKa = 11.84GLLLAFLLITVILTVTDD67 pKa = 4.34LCTGASGMTPVEE79 pKa = 4.0LVRR82 pKa = 11.84ALFGGGAAEE91 pKa = 3.79QRR93 pKa = 11.84MIVWRR98 pKa = 11.84IRR100 pKa = 11.84MPRR103 pKa = 11.84VLAALGAGCGLALSGCVMQNVLRR126 pKa = 11.84NPMASPSTLGVSNAAVLGANVAIILFGAGSFHH158 pKa = 6.27STHH161 pKa = 6.29GSEE164 pKa = 5.07LTLQKK169 pKa = 10.36PGLVTACAFLFALGATALVLLLSGRR194 pKa = 11.84HH195 pKa = 4.98RR196 pKa = 11.84FSAEE200 pKa = 3.9TVVLAGVALGALCSAGTTVLQYY222 pKa = 10.59MAMDD226 pKa = 3.96TQVAAAVFWTFGDD239 pKa = 4.0LGRR242 pKa = 11.84ASFKK246 pKa = 10.86EE247 pKa = 3.88SLLLIGVSGVCFVYY261 pKa = 9.44FFVRR265 pKa = 11.84GRR267 pKa = 11.84AYY269 pKa = 10.64DD270 pKa = 3.4AMGCGEE276 pKa = 4.14EE277 pKa = 4.35SARR280 pKa = 11.84SLGVPTGRR288 pKa = 11.84VRR290 pKa = 11.84CFTLLLASLLCAVCVSFLGIIGFVGLIAPQLARR323 pKa = 11.84RR324 pKa = 11.84MTGAGHH330 pKa = 7.35RR331 pKa = 11.84YY332 pKa = 9.23LLPATALTGASLLLFSDD349 pKa = 3.83TLSRR353 pKa = 11.84LLPFRR358 pKa = 11.84MTLPVGAVTSLFGAPVFLLLLLRR381 pKa = 11.84GRR383 pKa = 11.84KK384 pKa = 9.16EE385 pKa = 3.9GVRR388 pKa = 11.84RR389 pKa = 3.63

Molecular weight: 41.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2084

0

2084

624443

31

2372

299.6

33.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.222 ± 0.059

2.038 ± 0.028

5.904 ± 0.046

6.227 ± 0.045

4.237 ± 0.041

7.7 ± 0.053

2.003 ± 0.035

6.025 ± 0.05

4.937 ± 0.053

9.103 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.029

3.775 ± 0.033

3.854 ± 0.029

2.96 ± 0.033

5.872 ± 0.066

5.601 ± 0.046

5.928 ± 0.059

7.201 ± 0.044

0.891 ± 0.02

3.864 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski