Amino acid dipepetide frequency for Clostridium sp. CAG:448

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.551AlaAla: 9.551 ± 0.181	1.795AlaCys: 1.795 ± 0.054	6.696AlaAsp: 6.696 ± 0.114	6.343AlaGlu: 6.343 ± 0.12	4.032AlaPhe: 4.032 ± 0.107	7.551AlaGly: 7.551 ± 0.122	1.77AlaHis: 1.77 ± 0.048	4.825AlaIle: 4.825 ± 0.099	4.127AlaLys: 4.127 ± 0.105	9.178AlaLeu: 9.178 ± 0.142	2.269AlaMet: 2.269 ± 0.066	2.607AlaAsn: 2.607 ± 0.064	3.07AlaPro: 3.07 ± 0.07	3.193AlaGln: 3.193 ± 0.079	4.215AlaArg: 4.215 ± 0.104	4.412AlaSer: 4.412 ± 0.1	4.186AlaThr: 4.186 ± 0.092	8.105AlaVal: 8.105 ± 0.136	0.772AlaTrp: 0.772 ± 0.039	3.526AlaTyr: 3.526 ± 0.081	0.002AlaXaa: 0.002 ± 0.002
Cys	2.149CysAla: 2.149 ± 0.068	0.376CysCys: 0.376 ± 0.031	1.185CysAsp: 1.185 ± 0.042	1.131CysGlu: 1.131 ± 0.047	0.854CysPhe: 0.854 ± 0.04	2.284CysGly: 2.284 ± 0.071	0.42CysHis: 0.42 ± 0.025	1.14CysIle: 1.14 ± 0.048	0.822CysLys: 0.822 ± 0.038	1.568CysLeu: 1.568 ± 0.058	0.501CysMet: 0.501 ± 0.032	0.737CysAsn: 0.737 ± 0.035	0.93CysPro: 0.93 ± 0.041	0.468CysGln: 0.468 ± 0.034	1.443CysArg: 1.443 ± 0.05	1.022CysSer: 1.022 ± 0.043	1.188CysThr: 1.188 ± 0.058	1.419CysVal: 1.419 ± 0.052	0.135CysTrp: 0.135 ± 0.014	0.607CysTyr: 0.607 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	6.302AspAla: 6.302 ± 0.128	1.151AspCys: 1.151 ± 0.042	3.051AspAsp: 3.051 ± 0.078	3.565AspGlu: 3.565 ± 0.086	2.724AspPhe: 2.724 ± 0.068	5.823AspGly: 5.823 ± 0.112	1.023AspHis: 1.023 ± 0.041	3.488AspIle: 3.488 ± 0.086	2.553AspLys: 2.553 ± 0.077	4.739AspLeu: 4.739 ± 0.089	1.589AspMet: 1.589 ± 0.053	1.941AspAsn: 1.941 ± 0.058	2.405AspPro: 2.405 ± 0.064	1.396AspGln: 1.396 ± 0.057	3.779AspArg: 3.779 ± 0.081	3.193AspSer: 3.193 ± 0.083	3.536AspThr: 3.536 ± 0.082	3.759AspVal: 3.759 ± 0.081	0.569AspTrp: 0.569 ± 0.03	2.458AspTyr: 2.458 ± 0.071	0.0AspXaa: 0.0 ± 0.0
Glu	5.117GluAla: 5.117 ± 0.114	0.831GluCys: 0.831 ± 0.038	3.419GluAsp: 3.419 ± 0.072	5.163GluGlu: 5.163 ± 0.12	1.877GluPhe: 1.877 ± 0.065	3.858GluGly: 3.858 ± 0.09	1.175GluHis: 1.175 ± 0.045	4.297GluIle: 4.297 ± 0.096	4.878GluLys: 4.878 ± 0.117	4.929GluLeu: 4.929 ± 0.093	1.995GluMet: 1.995 ± 0.051	3.477GluAsn: 3.477 ± 0.086	1.821GluPro: 1.821 ± 0.062	2.348GluGln: 2.348 ± 0.07	3.712GluArg: 3.712 ± 0.084	3.222GluSer: 3.222 ± 0.079	3.778GluThr: 3.778 ± 0.092	3.571GluVal: 3.571 ± 0.079	0.615GluTrp: 0.615 ± 0.031	2.184GluTyr: 2.184 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	4.257PheAla: 4.257 ± 0.085	1.207PheCys: 1.207 ± 0.046	2.827PheAsp: 2.827 ± 0.071	2.071PheGlu: 2.071 ± 0.071	2.204PhePhe: 2.204 ± 0.077	3.416PheGly: 3.416 ± 0.083	0.849PheHis: 0.849 ± 0.045	1.976PheIle: 1.976 ± 0.059	1.281PheLys: 1.281 ± 0.047	4.135PheLeu: 4.135 ± 0.107	0.825PheMet: 0.825 ± 0.035	1.268PheAsn: 1.268 ± 0.044	1.693PhePro: 1.693 ± 0.055	0.886PheGln: 0.886 ± 0.043	2.679PheArg: 2.679 ± 0.074	3.27PheSer: 3.27 ± 0.078	2.434PheThr: 2.434 ± 0.071	2.972PheVal: 2.972 ± 0.079	0.413PheTrp: 0.413 ± 0.027	1.704PheTyr: 1.704 ± 0.064	0.0PheXaa: 0.0 ± 0.0
Gly	6.238GlyAla: 6.238 ± 0.112	1.782GlyCys: 1.782 ± 0.058	4.253GlyAsp: 4.253 ± 0.082	5.206GlyGlu: 5.206 ± 0.102	3.177GlyPhe: 3.177 ± 0.073	5.804GlyGly: 5.804 ± 0.127	1.4GlyHis: 1.4 ± 0.051	5.848GlyIle: 5.848 ± 0.112	5.354GlyLys: 5.354 ± 0.088	5.554GlyLeu: 5.554 ± 0.095	2.412GlyMet: 2.412 ± 0.064	3.512GlyAsn: 3.512 ± 0.077	1.161GlyPro: 1.161 ± 0.047	2.401GlyGln: 2.401 ± 0.073	3.731GlyArg: 3.731 ± 0.083	4.33GlySer: 4.33 ± 0.103	5.109GlyThr: 5.109 ± 0.109	5.712GlyVal: 5.712 ± 0.099	0.822GlyTrp: 0.822 ± 0.044	3.193GlyTyr: 3.193 ± 0.085	0.006GlyXaa: 0.006 ± 0.003
His	1.854HisAla: 1.854 ± 0.051	0.389HisCys: 0.389 ± 0.028	1.105HisAsp: 1.105 ± 0.047	0.926HisGlu: 0.926 ± 0.04	1.03HisPhe: 1.03 ± 0.045	1.629HisGly: 1.629 ± 0.052	0.536HisHis: 0.536 ± 0.047	1.435HisIle: 1.435 ± 0.059	0.759HisLys: 0.759 ± 0.031	1.888HisLeu: 1.888 ± 0.061	0.533HisMet: 0.533 ± 0.027	0.666HisAsn: 0.666 ± 0.037	1.172HisPro: 1.172 ± 0.04	0.466HisGln: 0.466 ± 0.031	1.243HisArg: 1.243 ± 0.05	0.898HisSer: 0.898 ± 0.04	1.347HisThr: 1.347 ± 0.049	1.252HisVal: 1.252 ± 0.048	0.167HisTrp: 0.167 ± 0.014	0.733HisTyr: 0.733 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.053IleAla: 6.053 ± 0.097	1.347IleCys: 1.347 ± 0.049	3.576IleAsp: 3.576 ± 0.092	2.999IleGlu: 2.999 ± 0.074	2.303IlePhe: 2.303 ± 0.067	5.229IleGly: 5.229 ± 0.11	1.089IleHis: 1.089 ± 0.043	3.072IleIle: 3.072 ± 0.095	2.312IleLys: 2.312 ± 0.074	5.903IleLeu: 5.903 ± 0.114	1.227IleMet: 1.227 ± 0.05	2.123IleAsn: 2.123 ± 0.067	3.172IlePro: 3.172 ± 0.081	1.408IleGln: 1.408 ± 0.051	4.423IleArg: 4.423 ± 0.093	3.779IleSer: 3.779 ± 0.084	3.578IleThr: 3.578 ± 0.084	4.14IleVal: 4.14 ± 0.09	0.535IleTrp: 0.535 ± 0.03	1.976IleTyr: 1.976 ± 0.054	0.003IleXaa: 0.003 ± 0.002
Lys	3.763LysAla: 3.763 ± 0.091	0.665LysCys: 0.665 ± 0.036	2.788LysAsp: 2.788 ± 0.075	3.797LysGlu: 3.797 ± 0.089	1.379LysPhe: 1.379 ± 0.055	3.196LysGly: 3.196 ± 0.083	0.846LysHis: 0.846 ± 0.038	3.188LysIle: 3.188 ± 0.069	4.037LysLys: 4.037 ± 0.111	3.973LysLeu: 3.973 ± 0.098	1.496LysMet: 1.496 ± 0.051	2.609LysAsn: 2.609 ± 0.066	1.813LysPro: 1.813 ± 0.062	1.738LysGln: 1.738 ± 0.051	2.899LysArg: 2.899 ± 0.079	2.522LysSer: 2.522 ± 0.067	3.325LysThr: 3.325 ± 0.087	2.891LysVal: 2.891 ± 0.075	0.424LysTrp: 0.424 ± 0.024	2.023LysTyr: 2.023 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	7.919LeuAla: 7.919 ± 0.121	2.862LeuCys: 2.862 ± 0.081	4.641LeuAsp: 4.641 ± 0.092	4.396LeuGlu: 4.396 ± 0.087	4.553LeuPhe: 4.553 ± 0.112	5.527LeuGly: 5.527 ± 0.106	2.228LeuHis: 2.228 ± 0.062	5.299LeuIle: 5.299 ± 0.121	3.301LeuLys: 3.301 ± 0.076	9.793LeuLeu: 9.793 ± 0.191	2.196LeuMet: 2.196 ± 0.061	2.903LeuAsn: 2.903 ± 0.061	4.462LeuPro: 4.462 ± 0.102	2.586LeuGln: 2.586 ± 0.065	5.927LeuArg: 5.927 ± 0.127	6.849LeuSer: 6.849 ± 0.111	5.639LeuThr: 5.639 ± 0.109	5.403LeuVal: 5.403 ± 0.11	0.817LeuTrp: 0.817 ± 0.039	3.727LeuTyr: 3.727 ± 0.084	0.003LeuXaa: 0.003 ± 0.002
Met	2.019MetAla: 2.019 ± 0.055	0.365MetCys: 0.365 ± 0.025	1.272MetAsp: 1.272 ± 0.049	1.731MetGlu: 1.731 ± 0.052	0.887MetPhe: 0.887 ± 0.037	1.734MetGly: 1.734 ± 0.061	0.594MetHis: 0.594 ± 0.036	1.741MetIle: 1.741 ± 0.055	1.781MetLys: 1.781 ± 0.053	2.583MetLeu: 2.583 ± 0.061	0.775MetMet: 0.775 ± 0.039	1.131MetAsn: 1.131 ± 0.04	1.262MetPro: 1.262 ± 0.046	1.027MetGln: 1.027 ± 0.036	1.754MetArg: 1.754 ± 0.059	1.523MetSer: 1.523 ± 0.047	1.806MetThr: 1.806 ± 0.055	1.501MetVal: 1.501 ± 0.045	0.189MetTrp: 0.189 ± 0.019	0.834MetTyr: 0.834 ± 0.042	0.003MetXaa: 0.003 ± 0.003
Asn	3.614AsnAla: 3.614 ± 0.088	0.729AsnCys: 0.729 ± 0.037	2.021AsnAsp: 2.021 ± 0.065	2.026AsnGlu: 2.026 ± 0.07	1.366AsnPhe: 1.366 ± 0.045	3.933AsnGly: 3.933 ± 0.09	0.806AsnHis: 0.806 ± 0.035	2.298AsnIle: 2.298 ± 0.06	1.595AsnLys: 1.595 ± 0.061	3.236AsnLeu: 3.236 ± 0.072	0.946AsnMet: 0.946 ± 0.038	1.443AsnAsn: 1.443 ± 0.055	2.01AsnPro: 2.01 ± 0.06	1.204AsnGln: 1.204 ± 0.053	2.442AsnArg: 2.442 ± 0.063	1.782AsnSer: 1.782 ± 0.057	2.264AsnThr: 2.264 ± 0.061	2.308AsnVal: 2.308 ± 0.058	0.354AsnTrp: 0.354 ± 0.029	1.374AsnTyr: 1.374 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	4.111ProAla: 4.111 ± 0.089	0.66ProCys: 0.66 ± 0.039	3.289ProAsp: 3.289 ± 0.081	3.286ProGlu: 3.286 ± 0.075	1.712ProPhe: 1.712 ± 0.054	2.758ProGly: 2.758 ± 0.075	0.876ProHis: 0.876 ± 0.041	2.016ProIle: 2.016 ± 0.056	1.376ProLys: 1.376 ± 0.05	3.176ProLeu: 3.176 ± 0.086	0.97ProMet: 0.97 ± 0.041	1.108ProAsn: 1.108 ± 0.044	1.094ProPro: 1.094 ± 0.043	1.289ProGln: 1.289 ± 0.051	1.625ProArg: 1.625 ± 0.064	1.978ProSer: 1.978 ± 0.054	2.048ProThr: 2.048 ± 0.062	3.379ProVal: 3.379 ± 0.076	0.287ProTrp: 0.287 ± 0.024	1.496ProTyr: 1.496 ± 0.057	0.002ProXaa: 0.002 ± 0.001
Gln	2.351GlnAla: 2.351 ± 0.067	0.528GlnCys: 0.528 ± 0.029	1.211GlnAsp: 1.211 ± 0.046	1.694GlnGlu: 1.694 ± 0.053	1.156GlnPhe: 1.156 ± 0.051	1.757GlnGly: 1.757 ± 0.055	0.516GlnHis: 0.516 ± 0.029	2.277GlnIle: 2.277 ± 0.062	2.309GlnLys: 2.309 ± 0.059	2.457GlnLeu: 2.457 ± 0.065	1.083GlnMet: 1.083 ± 0.044	1.682GlnAsn: 1.682 ± 0.062	1.161GlnPro: 1.161 ± 0.041	0.775GlnGln: 0.775 ± 0.039	1.739GlnArg: 1.739 ± 0.062	1.659GlnSer: 1.659 ± 0.048	2.023GlnThr: 2.023 ± 0.06	1.846GlnVal: 1.846 ± 0.048	0.259GlnTrp: 0.259 ± 0.025	1.113GlnTyr: 1.113 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	4.908ArgAla: 4.908 ± 0.117	1.023ArgCys: 1.023 ± 0.049	3.015ArgAsp: 3.015 ± 0.076	4.159ArgGlu: 4.159 ± 0.096	2.617ArgPhe: 2.617 ± 0.083	3.443ArgGly: 3.443 ± 0.081	1.332ArgHis: 1.332 ± 0.059	4.349ArgIle: 4.349 ± 0.092	3.413ArgLys: 3.413 ± 0.089	5.386ArgLeu: 5.386 ± 0.105	1.886ArgMet: 1.886 ± 0.063	2.452ArgAsn: 2.452 ± 0.064	1.698ArgPro: 1.698 ± 0.059	2.176ArgGln: 2.176 ± 0.075	3.879ArgArg: 3.879 ± 0.121	2.694ArgSer: 2.694 ± 0.076	3.355ArgThr: 3.355 ± 0.079	4.058ArgVal: 4.058 ± 0.079	0.46ArgTrp: 0.46 ± 0.031	2.42ArgTyr: 2.42 ± 0.067	0.0ArgXaa: 0.0 ± 0.0
Ser	5.988SerAla: 5.988 ± 0.096	1.063SerCys: 1.063 ± 0.043	3.637SerAsp: 3.637 ± 0.078	3.35SerGlu: 3.35 ± 0.088	2.61SerPhe: 2.61 ± 0.068	5.269SerGly: 5.269 ± 0.111	1.011SerHis: 1.011 ± 0.041	2.798SerIle: 2.798 ± 0.077	2.088SerLys: 2.088 ± 0.054	5.153SerLeu: 5.153 ± 0.109	1.544SerMet: 1.544 ± 0.047	1.725SerAsn: 1.725 ± 0.056	2.123SerPro: 2.123 ± 0.059	1.35SerGln: 1.35 ± 0.048	2.87SerArg: 2.87 ± 0.073	2.803SerSer: 2.803 ± 0.078	2.729SerThr: 2.729 ± 0.07	5.216SerVal: 5.216 ± 0.111	0.479SerTrp: 0.479 ± 0.034	2.21SerTyr: 2.21 ± 0.065	0.0SerXaa: 0.0 ± 0.0
Thr	5.92ThrAla: 5.92 ± 0.102	0.866ThrCys: 0.866 ± 0.06	4.386ThrAsp: 4.386 ± 0.084	3.97ThrGlu: 3.97 ± 0.098	2.388ThrPhe: 2.388 ± 0.074	5.166ThrGly: 5.166 ± 0.095	1.103ThrHis: 1.103 ± 0.042	2.727ThrIle: 2.727 ± 0.079	2.256ThrLys: 2.256 ± 0.068	5.607ThrLeu: 5.607 ± 0.11	1.196ThrMet: 1.196 ± 0.041	1.85ThrAsn: 1.85 ± 0.063	2.738ThrPro: 2.738 ± 0.076	1.531ThrGln: 1.531 ± 0.053	2.266ThrArg: 2.266 ± 0.06	2.679ThrSer: 2.679 ± 0.08	3.244ThrThr: 3.244 ± 0.123	6.515ThrVal: 6.515 ± 0.127	0.503ThrTrp: 0.503 ± 0.032	2.361ThrTyr: 2.361 ± 0.065	0.006ThrXaa: 0.006 ± 0.003
Val	5.94ValAla: 5.94 ± 0.109	1.858ValCys: 1.858 ± 0.062	3.751ValAsp: 3.751 ± 0.093	3.594ValGlu: 3.594 ± 0.081	3.438ValPhe: 3.438 ± 0.088	5.001ValGly: 5.001 ± 0.089	1.432ValHis: 1.432 ± 0.049	4.743ValIle: 4.743 ± 0.084	3.08ValLys: 3.08 ± 0.078	7.23ValLeu: 7.23 ± 0.14	1.85ValMet: 1.85 ± 0.053	2.524ValAsn: 2.524 ± 0.075	3.073ValPro: 3.073 ± 0.066	1.97ValGln: 1.97 ± 0.061	4.793ValArg: 4.793 ± 0.113	5.019ValSer: 5.019 ± 0.117	4.574ValThr: 4.574 ± 0.103	4.931ValVal: 4.931 ± 0.098	0.618ValTrp: 0.618 ± 0.031	2.593ValTyr: 2.593 ± 0.073	0.002ValXaa: 0.002 ± 0.002
Trp	0.538TrpAla: 0.538 ± 0.032	0.176TrpCys: 0.176 ± 0.021	0.488TrpAsp: 0.488 ± 0.03	0.565TrpGlu: 0.565 ± 0.032	0.437TrpPhe: 0.437 ± 0.029	0.711TrpGly: 0.711 ± 0.038	0.251TrpHis: 0.251 ± 0.023	0.567TrpIle: 0.567 ± 0.033	0.501TrpLys: 0.501 ± 0.032	0.86TrpLeu: 0.86 ± 0.041	0.312TrpMet: 0.312 ± 0.022	0.466TrpAsn: 0.466 ± 0.032	0.162TrpPro: 0.162 ± 0.015	0.437TrpGln: 0.437 ± 0.028	0.476TrpArg: 0.476 ± 0.033	0.476TrpSer: 0.476 ± 0.027	0.472TrpThr: 0.472 ± 0.03	0.527TrpVal: 0.527 ± 0.035	0.122TrpTrp: 0.122 ± 0.014	0.367TrpTyr: 0.367 ± 0.027	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.63TyrAla: 3.63 ± 0.086	0.7TyrCys: 0.7 ± 0.035	2.433TyrAsp: 2.433 ± 0.08	2.295TyrGlu: 2.295 ± 0.066	1.622TyrPhe: 1.622 ± 0.061	2.918TyrGly: 2.918 ± 0.081	0.772TyrHis: 0.772 ± 0.038	2.162TyrIle: 2.162 ± 0.056	1.547TyrLys: 1.547 ± 0.053	3.678TyrLeu: 3.678 ± 0.087	0.905TyrMet: 0.905 ± 0.035	1.529TyrAsn: 1.529 ± 0.059	1.515TyrPro: 1.515 ± 0.053	0.948TyrGln: 0.948 ± 0.043	2.82TyrArg: 2.82 ± 0.064	1.904TyrSer: 1.904 ± 0.062	2.641TyrThr: 2.641 ± 0.082	2.506TyrVal: 2.506 ± 0.069	0.375TyrTrp: 0.375 ± 0.024	1.744TyrTyr: 1.744 ± 0.066	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.002XaaCys: 0.002 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.002XaaHis: 0.002 ± 0.002	0.002XaaIle: 0.002 ± 0.001	0.002XaaLys: 0.002 ± 0.002	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.008XaaArg: 0.008 ± 0.003	0.0XaaSer: 0.0 ± 0.0	0.005XaaThr: 0.005 ± 0.002	0.002XaaVal: 0.002 ± 0.001	0.002XaaTrp: 0.002 ± 0.002	0.002XaaTyr: 0.002 ± 0.002	0.032XaaXaa: 0.032 ± 0.009

Statistics based on 2084 proteins (624443 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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