Streptomyces sp. FXJ1.172

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7600 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A176L1H9|A0A176L1H9_9ACTN Uncharacterized protein OS=Streptomyces sp. FXJ1.172 OX=710705 GN=A6P39_24725 PE=4 SV=1
MM1 pKa = 7.41TVQQEE6 pKa = 4.49APGGEE11 pKa = 4.07ALEE14 pKa = 4.15VWIDD18 pKa = 3.41QDD20 pKa = 3.99LCTGDD25 pKa = 5.01GICAQYY31 pKa = 10.9APEE34 pKa = 4.23VFEE37 pKa = 5.9LDD39 pKa = 3.05IDD41 pKa = 3.65GLAYY45 pKa = 10.73VKK47 pKa = 10.8GADD50 pKa = 4.46DD51 pKa = 6.0ALLQDD56 pKa = 4.48AGATVPVPLPLLTDD70 pKa = 3.6VVDD73 pKa = 4.12SAKK76 pKa = 10.15EE77 pKa = 3.93CPGEE81 pKa = 4.38CIHH84 pKa = 6.4VRR86 pKa = 11.84RR87 pKa = 11.84VSDD90 pKa = 3.27KK91 pKa = 10.85VEE93 pKa = 4.17VYY95 pKa = 10.9GPDD98 pKa = 3.36AEE100 pKa = 4.31

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A176LDT2|A0A176LDT2_9ACTN UPF0182 protein A6P39_00140 OS=Streptomyces sp. FXJ1.172 OX=710705 GN=A6P39_00140 PE=3 SV=1
MM1 pKa = 7.43TLTRR5 pKa = 11.84RR6 pKa = 11.84QGRR9 pKa = 11.84KK10 pKa = 9.12GRR12 pKa = 11.84VAAVLLTLLLGLGLAVTPAAAAPAAARR39 pKa = 11.84HH40 pKa = 4.68TTATTGTVRR49 pKa = 11.84AVTAFPGAASTIRR62 pKa = 11.84LRR64 pKa = 11.84RR65 pKa = 11.84TTSTHH70 pKa = 5.72HH71 pKa = 6.59ASAVHH76 pKa = 6.48LRR78 pKa = 11.84RR79 pKa = 11.84TKK81 pKa = 9.21TKK83 pKa = 10.24KK84 pKa = 10.19KK85 pKa = 10.7SFLKK89 pKa = 10.68KK90 pKa = 10.37LGIFLLVVVILVILFIVLVVWLLIHH115 pKa = 7.01LIRR118 pKa = 11.84RR119 pKa = 11.84AFRR122 pKa = 11.84GRR124 pKa = 11.84RR125 pKa = 3.38

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7600

0

7600

2447124

29

3545

322.0

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.716 ± 0.044

0.818 ± 0.008

5.796 ± 0.022

5.49 ± 0.031

2.747 ± 0.016

9.499 ± 0.026

2.413 ± 0.013

3.156 ± 0.019

2.175 ± 0.022

10.388 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.715 ± 0.011

1.794 ± 0.017

6.099 ± 0.029

2.942 ± 0.019

7.973 ± 0.034

5.029 ± 0.026

6.187 ± 0.032

8.372 ± 0.026

1.546 ± 0.012

2.144 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski