Pine marten torque teno virus 1
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9ITT1|G9ITT1_9VIRU Capsid protein OS=Pine marten torque teno virus 1 OX=1128423 PE=3 SV=1
MM1 pKa = 7.73 LDD3 pKa = 3.74 KK4 pKa = 10.43 WLQSCADD11 pKa = 3.85 THH13 pKa = 6.87 SLWCKK18 pKa = 8.29 CPEE21 pKa = 4.08 WRR23 pKa = 11.84 SHH25 pKa = 4.28 VPGWRR30 pKa = 11.84 GDD32 pKa = 3.67 TAGDD36 pKa = 3.68 GAAEE40 pKa = 4.24 LPEE43 pKa = 4.37 DD44 pKa = 3.72 VQVAFDD50 pKa = 3.46 IAFEE54 pKa = 5.17 DD55 pKa = 5.22 GADD58 pKa = 3.49 SDD60 pKa = 3.82 QPEE63 pKa = 3.94 RR64 pKa = 4.02
Molecular weight: 7.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.251
IPC2_protein 4.139
IPC_protein 4.062
Toseland 3.859
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.215
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.037
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 3.592
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.091
Protein with the highest isoelectric point:
>tr|G9ITT1|G9ITT1_9VIRU Capsid protein OS=Pine marten torque teno virus 1 OX=1128423 PE=3 SV=1
MM1 pKa = 7.37 AWRR4 pKa = 11.84 YY5 pKa = 6.49 RR6 pKa = 11.84 WRR8 pKa = 11.84 WRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 APRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 AGRR20 pKa = 11.84 FRR22 pKa = 11.84 YY23 pKa = 9.51 RR24 pKa = 11.84 FRR26 pKa = 11.84 RR27 pKa = 11.84 WRR29 pKa = 11.84 RR30 pKa = 11.84 LRR32 pKa = 11.84 PARR35 pKa = 11.84 KK36 pKa = 7.59 VRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 YY43 pKa = 9.22 RR44 pKa = 11.84 KK45 pKa = 8.93 RR46 pKa = 11.84 HH47 pKa = 4.97 FKK49 pKa = 10.42 KK50 pKa = 10.38 KK51 pKa = 10.19 AIKK54 pKa = 8.99 QWQPSHH60 pKa = 5.66 VARR63 pKa = 11.84 CIIRR67 pKa = 11.84 GWHH70 pKa = 5.42 IGFWGNKK77 pKa = 9.04 ANLSYY82 pKa = 10.61 PFQEE86 pKa = 3.87 WTRR89 pKa = 11.84 PTKK92 pKa = 10.44 EE93 pKa = 3.81 GEE95 pKa = 3.91 AGFFTIRR102 pKa = 11.84 EE103 pKa = 4.37 GGASLWHH110 pKa = 6.38 FNLSHH115 pKa = 7.66 FYY117 pKa = 11.07 RR118 pKa = 11.84 EE119 pKa = 4.04 FTFGRR124 pKa = 11.84 NTWSRR129 pKa = 11.84 SNEE132 pKa = 3.85 GFDD135 pKa = 3.16 LATYY139 pKa = 10.22 HH140 pKa = 5.1 GTKK143 pKa = 10.39 LIFYY147 pKa = 6.96 PHH149 pKa = 7.52 PLHH152 pKa = 7.27 NYY154 pKa = 8.03 IVWWWRR160 pKa = 11.84 DD161 pKa = 3.45 FGDD164 pKa = 3.81 VSAAQFKK171 pKa = 10.34 HH172 pKa = 5.0 SHH174 pKa = 5.81 PAKK177 pKa = 10.45 ALLEE181 pKa = 4.33 RR182 pKa = 11.84 NHH184 pKa = 6.06 FVIKK188 pKa = 10.34 SIPQGGRR195 pKa = 11.84 KK196 pKa = 7.8 KK197 pKa = 7.35 TTIFIPPPTTIQTGWSVMKK216 pKa = 10.38 KK217 pKa = 6.96 WANVNLFRR225 pKa = 11.84 LGMTMINTGNPLLHH239 pKa = 7.19 KK240 pKa = 10.53 GRR242 pKa = 11.84 LLAKK246 pKa = 10.27 VPIGTYY252 pKa = 9.08 WSTGNYY258 pKa = 9.76 RR259 pKa = 11.84 YY260 pKa = 10.14 FEE262 pKa = 4.61 DD263 pKa = 3.89 RR264 pKa = 11.84 LPVEE268 pKa = 4.48 TLPEE272 pKa = 4.2 ASTNIAYY279 pKa = 9.47 YY280 pKa = 9.74 SWWWDD285 pKa = 3.23 DD286 pKa = 3.61 SQDD289 pKa = 3.36 NKK291 pKa = 10.53 IAIRR295 pKa = 11.84 DD296 pKa = 3.88 NEE298 pKa = 4.0 NSNDD302 pKa = 3.53 GEE304 pKa = 4.82 TIFHH308 pKa = 6.66 LKK310 pKa = 8.86 IPYY313 pKa = 8.56 WLEE316 pKa = 3.46 FFGAGDD322 pKa = 3.51 QYY324 pKa = 11.31 IEE326 pKa = 4.11 QTKK329 pKa = 10.02 KK330 pKa = 10.84 VYY332 pKa = 9.49 IWWYY336 pKa = 10.36 RR337 pKa = 11.84 EE338 pKa = 3.61 DD339 pKa = 4.32 ASIIDD344 pKa = 3.71 YY345 pKa = 10.84 HH346 pKa = 8.1 SITSEE351 pKa = 4.87 HH352 pKa = 6.13 RR353 pKa = 11.84 KK354 pKa = 8.63 QWIKK358 pKa = 10.88 LGNSSQGSISTALLAWSNIGRR379 pKa = 11.84 HH380 pKa = 5.66 GPFMMAEE387 pKa = 4.06 EE388 pKa = 5.42 DD389 pKa = 3.38 ISNNSYY395 pKa = 11.57 SFFFKK400 pKa = 10.85 YY401 pKa = 9.91 KK402 pKa = 10.58 SYY404 pKa = 9.02 WRR406 pKa = 11.84 WGGVHH411 pKa = 6.18 PTPATNNDD419 pKa = 3.71 PVTVSPNSDD428 pKa = 3.44 DD429 pKa = 3.66 FAEE432 pKa = 4.33 VQVRR436 pKa = 11.84 NPLRR440 pKa = 11.84 VGRR443 pKa = 11.84 AALHH447 pKa = 6.29 PWDD450 pKa = 5.3 LDD452 pKa = 3.38 PDD454 pKa = 4.26 GFITTAKK461 pKa = 9.63 FRR463 pKa = 11.84 DD464 pKa = 3.66 MLGFSPSAITGLPPPIPQRR483 pKa = 11.84 RR484 pKa = 11.84 AKK486 pKa = 10.4 RR487 pKa = 11.84 SLSPSSGEE495 pKa = 4.1 SEE497 pKa = 4.24 EE498 pKa = 4.36 EE499 pKa = 4.36 GPSVRR504 pKa = 11.84 RR505 pKa = 11.84 RR506 pKa = 11.84 RR507 pKa = 11.84 SGDD510 pKa = 3.22 GEE512 pKa = 4.27 LEE514 pKa = 3.94 QNSFYY519 pKa = 10.99 FEE521 pKa = 4.62 EE522 pKa = 4.85 DD523 pKa = 3.28 TLPPYY528 pKa = 8.99 PTFPPGEE535 pKa = 4.04 TAEE538 pKa = 4.15 EE539 pKa = 4.43 KK540 pKa = 10.38 IRR542 pKa = 11.84 ALVEE546 pKa = 3.42 QLRR549 pKa = 11.84 RR550 pKa = 11.84 DD551 pKa = 3.26 RR552 pKa = 11.84 DD553 pKa = 3.24 KK554 pKa = 11.11 RR555 pKa = 11.84 RR556 pKa = 11.84 KK557 pKa = 8.1 LRR559 pKa = 11.84 KK560 pKa = 9.4 RR561 pKa = 11.84 IRR563 pKa = 11.84 SFLRR567 pKa = 11.84 DD568 pKa = 3.11
Molecular weight: 67.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.706
IPC_protein 10.409
Toseland 10.496
ProMoST 10.189
Dawson 10.643
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.891
Grimsley 10.701
Solomon 10.701
Lehninger 10.672
Nozaki 10.452
DTASelect 10.335
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.526
IPC_peptide 10.701
IPC2_peptide 9.107
IPC2.peptide.svr19 8.7
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
814
64
568
271.3
31.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.125 ± 1.574
0.86 ± 1.069
5.774 ± 2.257
6.511 ± 1.204
4.791 ± 1.364
7.371 ± 0.792
2.826 ± 0.447
4.791 ± 1.243
4.914 ± 0.841
6.265 ± 0.339
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.351 ± 0.136
3.317 ± 1.484
7.002 ± 0.681
3.808 ± 1.353
10.811 ± 1.533
7.002 ± 0.187
4.914 ± 0.67
3.194 ± 0.382
4.423 ± 0.718
2.948 ± 1.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here